Gene expression profiling in psoriatic scalp hair follicles: clobetasol propionate shampoo 0.05% normalizes psoriasis disease markers

Aubert, Jacques; Reiniche, Pascale; Fogel, Paul; Poulin, Yves; Lui, Harvey; Lynde, Charles; Shapiro, Jerry; Villemagne, Hervé; Soto, Pascale; Voegel, Johannes J.

doi:10.1111/j.1468-3083.2010.03637.x

Cited by 7 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[10][11][12] Noninvasive and minimally invasive means, including skin tape stripping, abrasion methods, reverse iontophoresis and hair root sampling, are therefore of increasing interest to access biochemical, molecular and genetic processes. [13][14][15][16] Recent publications report proteomic profiling of the stratum corneum (SC) in atopic dermatitis. [17][18][19] In psoriatic scales, although it has been known since pioneering work in the late 1970s that several chemokines are present, [20][21][22] only few studies report the parallel analysis of a number of chemokines and cytokines in psoriatic scales.…”

Section: What Does This Study Add?mentioning

confidence: 99%

“…Biomarker measurements in psoriasis are typically based on protein‐ or mRNA‐level quantification using invasive skin biopsy procedures, which can be associated with scarring, pain, risk of infection and allergic reactions . Noninvasive and minimally invasive means, including skin tape stripping, abrasion methods, reverse iontophoresis and hair root sampling, are therefore of increasing interest to access biochemical, molecular and genetic processes …”

mentioning

confidence: 99%

See 1 more Smart Citation

Noninvasive proteome analysis of psoriatic stratum corneum reflects pathophysiological pathways and is useful for drug profiling

Méhul

Séraïdaris

et al. 2017

Br J Dermatol

View full text Add to dashboard Cite

show abstract

Section: What Does This Study Add?mentioning

confidence: 99%

mentioning

confidence: 99%

Noninvasive proteome analysis of psoriatic stratum corneum reflects pathophysiological pathways and is useful for drug profiling

Méhul

Séraïdaris

et al. 2017

Br J Dermatol

View full text Add to dashboard Cite

show abstract

“…Clobetasol propionate (CP), a potent glucocorticoid, has been used in many different clinical trials and was shown to be effective for various inflammatory-caused scalp diseases, e.g. AA, LPP, FFA, and scalp psoriasis [4][5][6][7][8][9]. However, the described therapies are tedious for the patient as they entail application of an ointment to the desired site of action under occlusion with a plastic film, or application of a shampoo, cream, or foam several times a day for efficient treatment, all of which resulting in decreased patient compliance.…”

Section: Introductionmentioning

confidence: 99%

Nanocarriers for optimizing the balance between interfollicular permeation and follicular uptake of topically applied clobetasol to minimize adverse effects

Mathes

Melero

Conrad

et al. 2016

Journal of Controlled Release

View full text Add to dashboard Cite

The treatment of various hair disorders has become a central focus of good dermatologic patient care as it affects men and women all over the world. For many inflammatory-based scalp diseases, glucocorticoids are an essential part of treatment, even though they are known to cause systemic as well as local adverse effects when applied topically. Therefore, efficient targeting and avoidance of these side effects are of utmost importance. Optimizing the balance between drug release, interfollicular permeation, and follicular uptake may allow minimizing these adverse events and simultaneously improve drug delivery, given that one succeeds in targeting a sustained release formulation to the hair follicle. To test this hypothesis, three types of polymeric nanocarriers (nanospheres, nanocapsules, lipid-core nanocapsules) for the potent glucocorticoid Clobetasol propionate (CP) were prepared. They all exhibited a sustained release of drug, as was desired. The particles were formulated as a dispersion and hydrogel and (partially) labeled with Rhodamin B for quantification purposes. Follicular uptake was investigated using the Differential Stripping method and was found highest for nanocapsules in dispersion after application of massage. Moreover, the active ingredient (CP) as well as the nanocarrier (Rhodamin B labeled polymer) recovered in the hair follicle were measured simultaneously, revealing an equivalent uptake of both. In contrast, only negligible amounts of CP could be detected in the hair follicle when applied as free drug in solution or hydrogel, regardless of any massage. Skin permeation experiments using heat-separated human epidermis mounted in Franz Diffusion cells revealed equivalent reduced transdermal permeability for all nanocarriers in comparison to application of the free drug. Combining these results, nanocapsules formulated as an aqueous dispersion and applied by massage appeared to be a good candidate to maximize follicular targeting and minimize drug penetration into the interfollicular epidermis. We conclude that such nanotechnology-based formulations provide a viable strategy for more efficient -2-drug delivery to the hair follicle. Moreover, they present a way to minimize adverse effects of potent glucocorticoids by releasing the drug in a controlled manner and simultaneously decreasing interfollicular permeation, offering an advantage over conventional formulations for inflammatorybased skin/scalp diseases. Graphical Abstract AbbreviationsAA: Alopecia areata ANOVA: Analysis of variance CCT: capric/caprylic triglyceride CP: Clobetasol propionate

show abstract

“…The expressionof IL8 gene was induced by the irritants in bulge-derived keratinocytes (BDKs) 44 . IL8 expression was significantly increased in psoriatic hair follicles 45 . Altogether, these results suggest that IL8 might be involved in wool growth regulation.…”

Section: Analysis Of Highly Differentially Expressed Genes (>10-fold)mentioning

confidence: 92%

Discovery of genes and proteins possibly regulating mean wool fibre diameter using cDNA microarray and proteomic approaches

Zhao

Qin

Xin

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Wool fibre diameter (WFD) is one of the wool traits with higher economic impact. However, the main genes specifically regulating WFD remain unidentified. In this current work we have used Agilent Sheep Gene expression Microarray and proteomic technology to investigate the gene expression patterns of body side skin, bearing more wool, in Aohan fine wool sheep, a Chinese indigenous breed, and compared them with that of small tail Han sheep, a sheep bread with coarse wool. Microarray analyses showed that most of the genes likely determining wool diameter could be classified into a few categories, including immune response, regulation of receptor binding and growth factor activity. Certain gene families might play a role in hair growth regulation. These include growth factors, immune cytokines, solute carrier families, cellular respiration and glucose transport amongst others. Proteomic analyses also identified scores of differentially expressed proteins.

show abstract

Gene expression profiling in psoriatic scalp hair follicles: clobetasol propionate shampoo 0.05% normalizes psoriasis disease markers

Abstract: Hair follicles of SP patients are affected by the inflammatory process. The change in the expression level of inflammation-related genes correlates with the severity of the disease.

Cited by 7 publications

References 47 publications

Noninvasive proteome analysis of psoriatic stratum corneum reflects pathophysiological pathways and is useful for drug profiling

Noninvasive proteome analysis of psoriatic stratum corneum reflects pathophysiological pathways and is useful for drug profiling

Nanocarriers for optimizing the balance between interfollicular permeation and follicular uptake of topically applied clobetasol to minimize adverse effects

Discovery of genes and proteins possibly regulating mean wool fibre diameter using cDNA microarray and proteomic approaches

Contact Info

Product

Resources

About