Gene Expression Profiling of Adipose Tissues in Obesity Susceptible and Resistant Rats under a High Fat Diet

Joo, Jeong In; Yun, Jong Won

doi:10.1159/000327959

Cited by 25 publications

(15 citation statements)

References 52 publications

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“…2), protein spots were identified by MALDI-TOF-MS (Table 3), and database searches were performed with high confidence based on high score and sequence coverage. For the results, a total of 1,045 individual matched spots ranging in mass from 6 to 240 kDa between pH 3-10 were detected, and obtained WAT protein maps represented patterns that were similar to our previous results [26,27,28,34]. Fifty-nine spots were identified with statistically different intensities between the ND and OP groups.…”

Section: Resultssupporting

confidence: 80%

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Differential Protein Expression in White Adipose Tissue from Obesity-Prone and Obesity-Resistant Mice in Response to High Fat Diet and Anti-Obesity Herbal Medicines

Kim

Park²,

Choi³

et al. 2015

Cell Physiol Biochem

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Background: One of the most interesting issues in obesity research is why certain humans are obesity-prone (OP) while others are obesity-resistant (OR) upon exposure to a high-calorie diet. However, the pathways responsible for these phenotypic differences are still largely unknown. Methods: In order to discover marker molecules determining susceptibility and/or resistance to obesity in response to high fat diet (HFD) or anti-obesity herbal medicine (TH), we conducted comparative proteomic analysis of white adipose tissue (WAT) from OP, OR, as well as TH-treated mice. Results: OP mice fed HFD gained approximately 33% more body weight than OR mice, and TH significantly reduced body weight gain in HFD-fed mice by 30%. These mice were further subjected to proteomic analysis using two-dimensional electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Proteomic data revealed 59 spots that were differentially regulated from a total of 1,045 matched spots, and 57 spots of these were identified as altered WAT proteins between OP and OR mice by peptide mass finger printing. Interestingly, 45 proteins were similarly regulated in OR mice in response to TH treatment. Of these, 10 proteins have already been recognized in the context of obesity; however, other proteins involved in obesity susceptibility or resistance were identified for the first time in the present study. Conclusion: Our results suggest that TH actively contributed to body weight reduction in HFD-fed obese mice by altering protein regulation in WAT, and it was also found that TH-responsive proteins can be used as potent molecules for obesity treatment.

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Section: Resultssupporting

confidence: 80%

“…Obesity-related factors in adipose tissue proteins play major roles in the development of metabolic disorders [26,28]. Adipose tissue acts as an active endocrine organ, which secretes adipokines contributing to the regulation of physiological processes such as energy homeostasis, reproduction, and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Differential Protein Expression in White Adipose Tissue from Obesity-Prone and Obesity-Resistant Mice in Response to High Fat Diet and Anti-Obesity Herbal Medicines

Kim

Park²,

Choi³

et al. 2015

Cell Physiol Biochem

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“…The role of adipose tissue in etiology of obesity and its pathophysiologic changes is well recognized. Gene expression profiling of adipose tissues in OP and OR rats revealed a down-regulation in fatty acid oxidation genes in OP rats, while the OR rats were more resistant to these changes, suggesting a better utilization of lipids in OR animals [37]. Due to this characteristic difference of the strain, visceral fat deposits of OP rats were significantly higher than the OR and SD rats.…”

Section: Discussionmentioning

confidence: 91%

Treatment with low-dose resveratrol reverses cardiac impairment in obese prone but not in obese resistant rats

Louis

Thandapilly

Mohankumar

et al. 2012

The Journal of Nutritional Biochemistry

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We hypothesized that a low-dose resveratrol will reverse cardiovascular abnormalities in rats fed a high-fat (HF) diet. Obese prone (OP) and obese resistant (OR) rats were fed an HF diet for 17 weeks; Sprague-Dawley rats fed laboratory chow served as control animals. During the last 5 weeks of study, treatment group received resveratrol daily by oral gavage at a dosage of 2.5 mg/kg body weight. Assessments included echocardiography, blood pressure, adiposity, glycemia, insulinemia, lipidemia, and inflammatory and oxidative stress markers. Body weight and adiposity were significantly higher in OP rats when compared to OR rats. Echocardiographic measurements showed prolonged isovolumic relaxation time in HF-fed OP and OR rats. Treatment with resveratrol significantly improved diastolic function in OP but not in OR rats without affecting adiposity. OP and OR rats had increased blood pressure which remained unchanged with treatment. OP rats had elevated fasting serum glucose and insulin, whereas OR rats had increased serum glucose and normal insulin concentrations. Resveratrol treatment significantly reduced serum glucose while increasing serum insulin in both OP and OR rats. Inflammatory and oxidative stress markers, serum triglycerides and low-density lipoprotein were higher in OP rats, which were significantly reduced with treatment. In conclusion, HF induced cardiac dysfunction in both OP and OR rats. Treatment reversed abnormalities in diastolic heart function associated with HF feeding in OP rats, but not in OR rats. The beneficial effects of resveratrol may be mediated through regression of hyperglycemia, oxidative stress and inflammation.

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“…Factors that make some rats susceptible and others resistant to diet-induced obesity are still unclear. Microarray analysis revealed differences in expression of genes encoding metabolic enzymes in adipose tissue from OP and OR rats (13). Plasma proteome analysis showed changes related to inflammation and oxidative stress (14).…”

Section: Discussionmentioning

confidence: 99%

Cardiovascular, metabolic, and coronary dysfunction in high‐Fat‐Fed obesity‐resistant/prone rats

Dake

Oltman

2015

Obesity

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Objective: Obesity is a global epidemic leading to several comorbidities including diabetes and cardiovascular disease. The hypothesis that the genetic background of the obesity-prone rat (OP) predisposes to physiologic, metabolic, and microvascular dysfunction which is exacerbated by a diet high in saturated fats was tested. Methods: Male OP and obesity-resistant (OR) rats were fed either a diet containing 10% (chow) or 45% kcal fat (HF) for 42 weeks. Results: Weight of OP rats was greater than OR rats by 8 weeks on both diets. Blood pressure was increased in OP rats on chow and further augmented by HF diet compared to OR rats on similar diets. In contrast to weight and blood pressure, glucose clearance was similar in OR and OP rats on chow and impaired in both models on HF diet. Relaxation to acetylcholine was attenuated in OP rats compared to OR rats by 8 weeks and remained reduced throughout the study. A longer period of time was required to observe vascular dysfunction in HF-fed OR rats. Conclusions: When compared to OR rats, OP rats are prone to develop not only greater obesity but also hypertension and vascular dysfunction on a normal diet which is further augmented with HF diet.

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Gene Expression Profiling of Adipose Tissues in Obesity Susceptible and Resistant Rats under a High Fat Diet

Cited by 25 publications

References 52 publications

Differential Protein Expression in White Adipose Tissue from Obesity-Prone and Obesity-Resistant Mice in Response to High Fat Diet and Anti-Obesity Herbal Medicines

Differential Protein Expression in White Adipose Tissue from Obesity-Prone and Obesity-Resistant Mice in Response to High Fat Diet and Anti-Obesity Herbal Medicines

Treatment with low-dose resveratrol reverses cardiac impairment in obese prone but not in obese resistant rats

Cardiovascular, metabolic, and coronary dysfunction in high‐Fat‐Fed obesity‐resistant/prone rats

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