Gene Expression Profiling of Peripheral Blood Mononuclear Cells from Cattle Infected with<i>Mycobacterium paratuberculosis</i>

Coussens, Paul M.; Wiersma, Kacie; Abouzied, Amy; Sipkovsky, Sue

doi:10.1128/iai.70.10.5494-5502.2002

Cited by 94 publications

(76 citation statements)

References 28 publications

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“…Microarray technology allows simultaneous analysis of a vast number of genes, and may elucidate signaling pathways involved in AC mechanotransduction. In the present study we have used a recently developed bovine cDNA microarray [2] to determine how acute trauma of cartilage explants alters gene expression after 3 h. We also confirmed the differential expression of selected up-regulated and down-regulated genes identified from microarray analysis by quantitative real-time polymerase chain reaction (Q-RT-PCR). Expression of metalloproteinase genes known to be involved in cartilage catabolism in cytokine-stimulated models was also assessed with Q-RT-PCR.…”

Section: Introductionsupporting

confidence: 67%

Gene expression profile of mechanically impacted bovine articular cartilage explants

Chan

Schlueter

Coussens

et al. 2005

Journal Orthopaedic Research

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Traumatic injury to a joint can initiate cartilage degradation. Blunt trauma increases matrix damage and decreases proteoglycan synthesis in in vitro models. Few studies have investigated gene expression of articular cartilage (AC) following mechanical loading. Recent advances in microarray technology allow analysis of a number of genes, and may elucidate pathways of AC degradation. In the present study, we used a bovine cDNA microarray to determine how acute trauma of cartilage explants in the absence of underlying bone alters gene expression. Results indicate that at least 19 genes were differentially expressed at 3 h after trauma. Fourteen genes were up-regulated and five genes were down-regulated relative to control explants. The up-regulated genes included cytokine and chemokine receptors, enzymes, and molecules involved in signal transduction. Genes of adhesion molecules and apoptosis were down-regulated. The results of this study highlight the potential benefits of using a bovine cDNA microarray to study cartilage metabolism.

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Section: Introductionsupporting

confidence: 67%

Gene expression profile of mechanically impacted bovine articular cartilage explants

Chan

Schlueter

Coussens

et al. 2005

Journal Orthopaedic Research

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“…paratuberculosis infection on the production of both regulatory cytokines (IL-10 and TGF-␤). One study recently reported higher IL-10 gene expression in PBMC isolated from cows with clinical paratuberculosis than in cells from healthy animals (6). In addition, upregulation of IL-10 gene expression was also noted in monocyte-derived macrophages obtained from healthy animals after stimulation with live M. avium subsp.…”

Section: Discussionmentioning

confidence: 92%

Effects of Gamma Interferon, Interleukin-10, and Transforming Growth Factor β on the Survival ofMycobacterium aviumsubsp.paratuberculosisin Monocyte-Derived Macrophages from Naturally Infected Cattle

Khalifeh

Stabel

2004

Infect Immun

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Gamma interferon (IFN-␥) plays a significant role in the control of mycobacterial infections, includingMycobacterium avium subsp. paratuberculosis. However, the contribution of other immunoregulatory cytokines, such as interleukin-10 (IL-10) and transforming growth factor ␤ (TGF-␤), in Johne's disease has not been investigated as yet. In this study, we examined the effects of in vivo and in vitro infection with M. avium subsp. paratuberculosis on the production of IFN-␥, IL-10, and TGF-␤ by peripheral blood mononuclear cells (PBMC). We also examined the effects of exogenous IFN-␥, IL-10, and TGF-␤ on M. avium subsp. paratuberculosis survival in the cell cultures. PBMC obtained from naturally infected cows, regardless of their disease status, specifically upregulated IL-10 and TGF-␤ in culture supernatants in response to stimulation with live M. avium subsp. paratuberculosis. Nonstimulated PBMC recovered from subclinically infected animals secreted the lowest levels of TGF-␤, but after stimulation with live M. avium subsp. paratuberculosis, TGF-␤ levels in the culture supernatants increased to levels similar to that produced by PBMC from healthy animals. The numbers of viable M. avium subsp. paratuberculosis recovered from cultures from naturally infected animals were higher than those from healthy cows after in vitro infection with M. avium subsp. paratuberculosis. The addition of exogenous IL-10 and TGF-␤ to PBMC isolated from healthy cows inhibited the bactericidal activity of these cells as evidenced by the increased number of viable M. avium subsp. paratuberculosis recovered from these cultures compared to cell cultures containing medium alone. These data suggest important immune regulatory roles for IL-10 and TGF-␤ during infection with M. avium subsp. paratuberculosis that may be directly related to their effects on macrophage activation and killing of M. avium subsp. paratuberculosis.

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“…paratuberculosis antigens. Recent gene profiling has revealed changes in gene expression that could account for alterations in cell signaling mediated through cell receptors and altered patterns of cytokine and chemokine secretion (12,13). Significant changes were noted in expression of multiple genes in cultures of PBMC from infected animals incubated with live M. avium subsp.…”

Section: Analysis Of Cd8mentioning

confidence: 99%

“…Total RNA from bovine cecal lymph nodes was prepared using TRIzol reagent (Invitrogen, Carlsbad, Calif.) according to the manufacturer's instructions. The first-strand cDNA was prepared using the SuperScript first-strand synthesis system for RT-PCR (Invitrogen) with oligo(dT) [12][13][14][15][16][17][18] as a primer. The PCR mixture contained 1ϫ PCR buffer, a 0.2 mM concentration of each deoxynucleoside triphosphate, a 0.1 M concentration each of the IS900-specific forward (5Ј-CCGCTAATTG AGAGATGCGATTGG-3Ј) and reverse (5Ј-AATCAACTCCAGCAGCGCGG CCTCG-3Ј) primers, 1.25 U of Taq DNA polymerase (Invitrogen), and 1 l of first-strand cDNA template.…”

Section: Methodsmentioning

confidence: 99%

Analysis of the Immune Response toMycobacterium aviumsubsp.paratuberculosisin Experimentally Infected Calves

Koo

Park

Hamilton³

et al. 2004

Infect Immun

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Johne's disease of cattle is widespread and causes significant economic loss to producers. Control has been hindered by limited understanding of the immune response to the causative agent, Mycobacterium avium subsp. paratuberculosis, and lack of an effective vaccine and sensitive specific diagnostic assays. The present study was conducted to gain insight into factors affecting the immune response to M. avium subsp.

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Gene Expression Profiling of Peripheral Blood Mononuclear Cells from Cattle Infected withMycobacterium paratuberculosis

Cited by 94 publications

References 28 publications

Gene expression profile of mechanically impacted bovine articular cartilage explants

Gene expression profile of mechanically impacted bovine articular cartilage explants

Effects of Gamma Interferon, Interleukin-10, and Transforming Growth Factor β on the Survival ofMycobacterium aviumsubsp.paratuberculosisin Monocyte-Derived Macrophages from Naturally Infected Cattle

Analysis of the Immune Response toMycobacterium aviumsubsp.paratuberculosisin Experimentally Infected Calves

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