Gene expression profiling of the developing Drosophila CNS midline cells

Kearney, Joseph B.; Wheeler, Scott R.; Estes, Patricia A.; Parente, Beth; Crews, Stephen T.

doi:10.1016/j.ydbio.2004.08.047

Cited by 90 publications

(117 citation statements)

References 77 publications

Supporting

Mentioning

110

Contrasting

Unclassified

Order By: Relevance

“…Acf1 contains two PHD fingers and a bromodomain, and it associates with Imitation of Switch (ISWI), a homolog of yeast SWI2/SNF2 and a small subunit of ACF (22,23). The expression of Acf1 was observed in a subset of cells in the midline primordium region of the CNS (24). Embryos injected with Acf1 dsRNA exhibited missing segments (Fig.…”

Section: Genes With Previously Uncharacterized Nervous System Phenotymentioning

confidence: 99%

RNA interference screen to identify genes required for Drosophila embryonic nervous system development

Koizumi

Higashida

Yoo

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

RNA interference (RNAi) has been shown to be a powerful method to study the function of genes in vivo by silencing endogenous mRNA with double-stranded (ds) RNA. Previously, we performed in vivo RNAi screening and identified 43 Drosophila genes, including 18 novel genes required for the development of the embryonic nervous system. In the present study, 22 additional genes affecting embryonic nervous system development were found. Novel RNAiinduced phenotypes affecting nervous system development were found for 16 of the 22 genes. Seven of the genes have unknown functions. Other genes found encode transcription factors, a chromatin-remodeling protein, membrane receptors, signaling molecules, and proteins involved in cell adhesion, RNA binding, and ion transport. Human orthologs were identified for proteins encoded by 16 of the genes. The total number of dsRNAs that we have tested for an RNAi-induced phenotype affecting the embryonic nervous system, including our previous study, is 7,312, which corresponds to Ϸ50% of the genes in the Drosophila genome.high-throughput screen ͉ neural development ͉ neural mutants

show abstract

Section: Genes With Previously Uncharacterized Nervous System Phenotymentioning

confidence: 99%

RNA interference screen to identify genes required for Drosophila embryonic nervous system development

Koizumi

Higashida

Yoo

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Embryo collection, in situ hybridization, immunostaining and confocal imaging were performed as previously described (Kearney et al, 2004;Wheeler et al, 2006;Wheeler et al, 2008). Digoxygenin-labeled or biotinlabeled antisense RNA probes for 5-HT1A, BarH1, CG13565, CG15236, CG16778, DAT, dgk, Glu-RI, NPFR1, nubbin, ple, pdm2, Tbh, wor, wrapper and VGlut for in situ hybridization were generated from cDNA clones from the Drosophila Gene Collection (Open Biosystems).…”

Section: In Situ Hybridization Immunostaining Microscopy and Statimentioning

confidence: 99%

“…The proneural bHLH genes play important roles in CNS and sensory cell development (Bertrand et al, 2002). Previous work has shown that l(1)sc was expressed in midline cells (Bossing and Brand, 2006;Kearney et al, 2004) and we earlier described the expression of ase in MPs and MNB (Wheeler et al, 2006). However, detailed expression analysis of the ac, l(1)sc and sc genes had not been carried out.…”

Section: As-c Genes Are Expressed In Midline Precursors and Their Neumentioning

confidence: 99%

Dual role for Drosophila lethal of scute in CNS midline precursor formation and dopaminergic neuron and motoneuron cell fate

2011

Self Cite

View full text Add to dashboard Cite

SUMMARYDopaminergic neurons play important behavioral roles in locomotion, reward and aggression. The Drosophila H-cell is a dopaminergic neuron that resides at the midline of the ventral nerve cord. Both the H-cell and the glutamatergic H-cell sib are the asymmetric progeny of the MP3 midline precursor cell. H-cell sib cell fate is dependent on Notch signaling, whereas H-cell fate is Notch independent. Genetic analysis of genes that could potentially regulate H-cell fate revealed that the lethal of scute [l(1)sc], tailup and SoxNeuro transcription factor genes act together to control H-cell gene expression. The l(1)sc bHLH gene is required for all H-cell-specific gene transcription, whereas tailup acts in parallel to l(1)sc and controls genes involved in dopamine metabolism. SoxNeuro functions downstream of l(1)sc and controls expression of a peptide neurotransmitter receptor gene. The role of l(1)sc may be more widespread, as a l(1)sc mutant shows reductions in gene expression in non-midline dopaminergic neurons. In addition, l(1)sc mutant embryos possess defects in the formation of MP4-6 midline precursor and the median neuroblast stem cell, revealing a proneural role for l(1)sc in midline cells. The Notch-dependent progeny of MP4-6 are the mVUM motoneurons, and these cells also require l(1)sc for mVUM-specific gene expression. Thus, l(1)sc plays an important regulatory role in both neurogenesis and specifying dopaminergic neuron and motoneuron identities.

show abstract

“…The gene was named mes2, based on its expression in the early mesoderm, and we shall henceforth refer to it by this name. mes2 has since been identified in several additional microarraybased screens that examined transcript levels in the wing imaginal disc (Butler et al, 2003), glia (Freeman et al, 2003), and the central nervous system (CNS) midline (Kearney et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Mes2, a MADF‐containing transcription factor essential for Drosophila development

Zimmermann

Furlong

Suyama

et al. 2006

Developmental Dynamics

View full text Add to dashboard Cite

The development of the Drosophila mesoderm is initiated by the basic helix-loop-helix transcription factor twist. We identified a gene encoding a putative transcription factor, mes2, in a screen for essential mesoderm-expressed genes that function downstream of twist. Mes2 protein belongs to a family of 48 Drosophila proteins containing MADF domains. MADF domains exist in worms, flies, and fish. Mes2 is a nuclear protein first produced in trunk and head mesoderm during late gastrulation. At later embryonic stages, mes2 is expressed in glia of the central and peripheral nervous systems, and in tissues derived from the head mesoderm. We have identified a null mutation of mes2 that leads to developmental arrest in first instar larvae. Increased production of Mes2 in multiple embryonic and larval tissues almost always causes lethality. The ubiquitous or epidermal misexpression of mes2 in the embryo causes a dramatic loss of epidermal integrity resulting in the failure of dorsal closure. Our data show that the precise regulation of mes2 expression is critical for normal development in Drosophila and implicate Mes2 in the regulation of essential target genes. Developmental Dynamics 235:3387-3395, 2006.

show abstract

Gene expression profiling of the developing Drosophila CNS midline cells

Cited by 90 publications

References 77 publications

RNA interference screen to identify genes required for Drosophila embryonic nervous system development

RNA interference screen to identify genes required for Drosophila embryonic nervous system development

Dual role for Drosophila lethal of scute in CNS midline precursor formation and dopaminergic neuron and motoneuron cell fate

Mes2, a MADF‐containing transcription factor essential for Drosophila development

Contact Info

Product

Resources

About