Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

Litvinova, L. S.; Yurova, K. A.; Шуплецова, В. В.; Хазиахматова, О. Г.; Малащенко, В. В.; Shunkin, E. O.; Melashchenko, E. S.; Тодосенко, Н. М.; Khlusova, M. Yu.; Sharkeev, Yurii P.; Комарова, Е. Г.; Sedelnikova, M. B.; Хлусов, И. А.

doi:10.3390/ijms21207682

Cited by 8 publications

(7 citation statements)

References 70 publications

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“…The osteogenic differentiation of hAMSCs on the plastic around the CaP-coated Ti alloys was determined after culturing for 21 days as previously described [32]. Osteogenic supplements (β-glycerophosphate, ascorbic acid, and dexamethasone) were not added to the culture medium because the self-differentiation osteogenic activity of hAMSCs in plastic wells initiated by the samples was determined.…”

Section: Estimation Of In Vitro Osteogenic Features Of Test Samples With Alizarin Red Stainingmentioning

confidence: 99%

“…Differentiated hAMSCs were stained with 2% water solution of Alizarin Red S (ARS; Sigma-Aldrich, St. Louis, MO, USA), as described in our numerous studies [27,28,32], to identify mineralization of the extracellular matrix (ECM) produced by osteoblasts as recommended by the International Society for Cellular Therapy (ISCT). All staining procedures were performed as recommended by the manufacturer, including use of a concentration gradient of ARS.…”

Section: Estimation Of In Vitro Osteogenic Features Of Test Samples With Alizarin Red Stainingmentioning

confidence: 99%

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Zn-Doped CaP-Based Coatings on Ti–6Al–4V and Ti–6Al–7Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities

Prosolov

Mitrichenko²,

Просолов³

et al. 2021

Coatings

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New TiNb-based alloys, such as Ti–6Al–7Nb, are currently being studied around the world as an alternative to other Ti alloys, e.g., instead of Ti–6Al–4V. We conducted a pilot study where thin (approximately 1.2 micron) CaP coatings containing low doses of Zn2+ (0.4–0.8 wt.%) were prepared by the radio frequency magnetron sputtering (RFMS) of Zn-hydroxyapatite (HA) target on Ti–6Al–4V and Ti–6Al–7Nb substrates and investigated their physicochemical properties, in vitro solubility, cytotoxicity, and antibacterial and osteogenic activities. The thickness of the obtained coatings was approximately 1.2–1.3 microns. Zn substitution did not result in roughness or structural or surface changes in the amorphous CaP coatings. The distributions of Ca, P, and Zn were homogeneous across the film thickness as shown by the EDX mapping of these elements. Zn doping of CaP coatings on both types of Ti-based alloys statistically influenced the results of the scratch-test. However, obtained values are satisfactory to use Zn-CaP coatings on biomedical implants. Increased Zn2+ release vs. tapered output of Ca and phosphate ions occurred during 5 weeks of an in vitro immersion test in 0.9% NaCl solution. Ti–6Al–7Nb alloy, unlike Ti–6Al–4V, promoted more linear biodegradation of CaP coatings in vitro. As a result, CaP-based surfaces on Ti–6Al–7Nb, compared with on Ti–6Al–4V alloy, augmented the total areas of Alizarin red staining in a 21-day culture of human adipose-derived mesenchymal stem cells in a statistically significant manner. Moreover, Zn–CaP coatings statistically reduced leukemic Jurkat T cell survival within 48 h of in vitro culture. Along with the higher solubility of the Zn–CaP surface, a greater reduction (4- to 5.5-fold) in Staphylococcus aureus growth was observed in vitro when 7-day extracts of the coatings were added into the microbial culture. Hence, Zn–CaP-coated Ti–6Al–7Nb alloy with controllable biodegradation as prepared by RFMS is a prospective material suitable for bone applications in cases where there is a risk of bacterial contamination with severe consequences, for example, in leukemic patients. Further research is needed to closely investigate the mechanical features and pathways of their solubility and antimicrobial, antitumor, and osteogenic activities.

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Section: Estimation Of In Vitro Osteogenic Features Of Test Samples With Alizarin Red Stainingmentioning

confidence: 99%

Section: Estimation Of In Vitro Osteogenic Features Of Test Samples With Alizarin Red Stainingmentioning

confidence: 99%

Zn-Doped CaP-Based Coatings on Ti–6Al–4V and Ti–6Al–7Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities

Prosolov

Mitrichenko²,

Просолов³

et al. 2021

Coatings

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“…Other parameters can also be considered in order to study the effects of biomaterials on cell biology. To evaluate if the biomaterial also maintains the immunophenotype of the seeded cells, the expression of specific cell markers (for example, CD73, CD90, and CD105 for mesenchymal stromal cells) can be assessed by flow cytometry [ 104 ].…”

Section: Biomaterials Biocompatibilitymentioning

confidence: 99%

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Pietro

Palmieri

Papi

et al. 2022

IJMS

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In the last 20 years, bone regenerative research has experienced exponential growth thanks to the discovery of new nanomaterials and improved manufacturing technologies that have emerged in the biomedical field. This revolution demands standardization of methods employed for biomaterials characterization in order to achieve comparable, interoperable, and reproducible results. The exploited methods for characterization span from biophysics and biochemical techniques, including microscopy and spectroscopy, functional assays for biological properties, and molecular profiling. This review aims to provide scholars with a rapid handbook collecting multidisciplinary methods for bone substitute R&D and validation, getting sources from an up-to-date and comprehensive examination of the scientific landscape.

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“…According to the previously described scheme [50], the analysis of multiplex polymerase chain reaction (PCR) in triplicate was carried out. For this, special TaqMan probes and primers at a concentration of 10 pM (Beagle, Moscow, Russia), as well as qPCRmixHS reagents (Evrogen, Russia), were used.…”

Section: Gene Expression In Jurkat T Cellsmentioning

confidence: 99%

Amorphous–Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies

et al. 2021

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A modern trend in traumatology, orthopedics, and implantology is the development of materials and coatings with an amorphous–crystalline structure that exhibits excellent biocopatibility. The structure and physico–chemical and biological properties of calcium phosphate (CaP) coatings deposited on Ti plates using the micro-arc oxidation (MAO) method under different voltages (200, 250, and 300 V) were studied. Amorphous, nanocrystalline, and microcrystalline statesof CaHPO4 and β-Ca2P2O7were observed in the coatings using TEM and XRD. The increase in MAO voltage resulted in augmentation of the surface roughness Ra from 2.5 to 6.5 µm, mass from 10 to 25 mg, thickness from 50 to 105 µm, and Ca/P ratio from 0.3 to 0.6. The electrical potential (EP) of the CaP coatings changed from −456 to −535 mV, while the zeta potential (ZP) decreased from −53 to −40 mV following an increase in the values of the MAO voltage. Numerous correlations of physical and chemical indices of CaP coatings were estimated. A decrease in the ZP magnitudes of CaP coatings deposited at 200–250 V was strongly associated with elevated hTERT expression in tumor-derived Jurkat T cells preliminarily activated with anti-CD2/CD3/CD28 antibodies and then contacted in vitro with CaP-coated samples for 14 days. In turn, in vitro survival of CD4+ subsets was enhanced, with proinflammatory cytokine secretion of activated Jurkat T cells. Thus, the applied MAO voltage allowed the regulation of the physicochemical properties of amorphous–crystalline CaP-coatings on Ti substrates to a certain extent. This method may be used as a technological mechanism to trigger the behavior of cells through contact with micro-arc CaP coatings. The possible role of negative ZP and Ca2+ as effectors of the biological effects of amorphous–crystalline CaP coatings is discussed. Micro-arc CaP coatings should be carefully tested to determine their suitability for use in patients with chronic lymphoid malignancies.

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Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

Cited by 8 publications

References 70 publications

Zn-Doped CaP-Based Coatings on Ti–6Al–4V and Ti–6Al–7Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities

Zn-Doped CaP-Based Coatings on Ti–6Al–4V and Ti–6Al–7Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Amorphous–Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies

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