Gene expression screening of human mast cells and eosinophils using high-density oligonucleotide probe arrays: abundant expression of major basic protein in mast cells

Nakajima, Toshiharu; Matsumoto, Kenji; Suto, Hajime; Tanaka, Kazuaki; Ebisawa, Motohiro; Tomita, Hisashi; Yuki, Keisuke; Katsunuma, Toshio; Akasawa, Akira; Hashida, Ryoichi; Sugita, Yuji; Ogawa, Hideoki; Ra, Chisei; Saito, Hirohisa

doi:10.1182/blood.v98.4.1127

Cited by 86 publications

(81 citation statements)

References 44 publications

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“…Small amounts of EDN and ECP were also found in neutrophils (Nakajima et al 2001). In this study, the mean percentage of other cells (lymphocytes or basophils) was 0.54 ± 0.60%, which was relatively low compared with proportions of other inflammatory cells.…”

Section: Discussionmentioning

confidence: 92%

Expression Levels of Eosinophil Granule Protein mRNAs in Induced Sputum Reflect Airway Hyperresponsiveness and Airflow Limitation

Jung

Kang

Lee

et al. 2014

Tohoku J. Exp. Med.

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Eosinophils are regarded as the major effector cells that produce symptoms in allergic diseases. Activation of eosinophils induces extracellular release of a number of eosinophil granule proteins, including major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil peroxidase (EPO), and eosinophilderived neurotoxin. The objective of this study was to evaluate the differences and significance of the sputum eosinophil% and expression levels of eosinophilic granule protein mRNAs in allergic airway disease. Induced sputum samples were obtained from non-smokers with 25 asthma, 54 eosinophilic bronchitis, 16 allergic rhinitis, and 19 healthy control subjects. The eosinophil granule protein mRNAs were measured with real time RT-PCR. There was no correlation between the sputum eosinophil% and the mRNA level of any of eosinophil granule proteins. However, the expression levels of MBP and ECP mRNAs were higher in subjects with each of the specified allergic diseases than those in control subjects (P < 0.05). Moreover, in the subjects with allergic sensitization, the expression levels of MBP and EPO mRNAs were significantly higher in those with airway hyperresponsiveness (13 subjects) than in those without airway hyperresponsiveness (32 subjects) (P = 0.004 and 0.010, respectively). In asthma patients, the FEV1% was negatively correlated with ECP mRNA levels (r = −0.510, P = 0.022), but showed no correlation with sputum eosinophil%. In conclusion, mRNA levels of eosinophil granule proteins, rather than sputum eosinophil%, may reflect airway hyperresponsiveness and airflow limitation. In practice, consideration for the eosinophil% as well as the eosinophil granule proteins levels in induced sputum is needed.

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Section: Discussionmentioning

confidence: 92%

Expression Levels of Eosinophil Granule Protein mRNAs in Induced Sputum Reflect Airway Hyperresponsiveness and Airflow Limitation

Jung

Kang

Lee

et al. 2014

Tohoku J. Exp. Med.

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“…28,30,31 Total RNA (0.1-10 g) was extracted from approximately 10 5 to 10 7 cells. Double-stranded cDNA was synthesized, and the cDNA was subjected to in vitro transcription in the presence of biotinylated nucleoside triphosphates.…”

Section: Genechip Expression Analysismentioning

confidence: 99%

Identification of specific gene expression profiles in human mast cells mediated by Toll-like receptor 4 and FcϵRI

Okumura

Kashiwakura

Tomita

et al. 2003

Blood

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139

119

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“…It is a well-known phenomenon that eosinophil becomes invisible after degranulation. Nakajima et al [4], however detected abundant immunoreactive protein and mRNA of MBP in MC granules. Expression of This indicates that MC may undergo phenotypic changes and synthesize MBP.…”

Section: Discussionmentioning

confidence: 99%

“…MBP is implicated in many pathological conditions including allergic reactions and parasitic infections, as well as activation and stimulation of cells to produce cytokines. Recently, Nakajima et al [4] demonstrated presence of abundant MBP in mast cells (MCs) and ranked it 4 th in quantity among other proteins. herefore, according to this inding, the use of (MBP) eosinophil-speciic is probably invalid.…”

Section: Introductionmentioning

confidence: 99%

Expression of Mast Cell Major Basic Protein in Oral Squamous Cell Carcinoma

Alkhabuli¹

2013

J Cytol Histol

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Role of mast cells and eosinophils in oral tumours is not yet well deine. Good and poor outcomes have been associated with eosinophils and mast cell abundant oral tumours. Major basic protein is one of the principal and speciic proteins present in eosinophils. It is implicated in many pathological conditions including allergic reactions, parasitic infections and activation and stimulation of cells to produce cytokines.The protein was thought to be exclusively present in the eosinophil granules. However, a recent study revealed its presence in mast cells as well.In this brief article, the authors conirm the presence of major basic protein in mast cells associated with oral squamous cell carcinoma.This inding may doubts the validity of using anti-major basic protein as eosinophil speciic. Furthermore, labelling eosinophils using anti-major basic protein antibodies as a marker would mistake mast cells as eosinophils.More importantly, the inding will better aid in understanding some of the mysterious function of MC in tumours.

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Gene expression screening of human mast cells and eosinophils using high-density oligonucleotide probe arrays: abundant expression of major basic protein in mast cells

Cited by 86 publications

References 44 publications

Expression Levels of Eosinophil Granule Protein mRNAs in Induced Sputum Reflect Airway Hyperresponsiveness and Airflow Limitation

Expression Levels of Eosinophil Granule Protein mRNAs in Induced Sputum Reflect Airway Hyperresponsiveness and Airflow Limitation

Identification of specific gene expression profiles in human mast cells mediated by Toll-like receptor 4 and FcϵRI

Expression of Mast Cell Major Basic Protein in Oral Squamous Cell Carcinoma

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