Gene mapping in the rat by mouse-rat somatic cell hybridization: synteny of the albumin and α-fetoprotein genes and assignment to chromosome 14

Szpirer, Josiane; Levan, Göran; Thörn, Magnus; Szpirer, Claude

doi:10.1159/000132047

Cited by 120 publications

(34 citation statements)

References 13 publications

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“…When testing for dominance of an allele at a given locus, one-way ANOVA was done followed by a contrast that compared the average value ofthe two homozygotes to that ofthe heterozy- Chromosome localization using rat-mouse somatic hybrids. Chromosome localization was done using PCR reactions on DNA of ratmouse somatic cell hybrids obtained from Szpirer et al (30).…”

Section: Methodsmentioning

confidence: 99%

Genetic mapping of two new blood pressure quantitative trait loci in the rat by genotyping endothelin system genes.

Deng¹,

Dene²,

Pravenec³

et al. 1994

J. Clin. Invest.

121

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The endothelin system, consisting of a series of potent vasoconstrictor peptides and their receptors, is potentially important in the control of blood pressure. We found that the gene coding for endothelin-2 (ET2), also known as vasoactive intestine peptide, cosegregated strongly with systolic blood pressure in a F2 population [F2(S x LEW)j derived from a cross of the Dahl salt-sensitive (S) rat and the Lewis (LEW/NCrIBR) (LEW) rat. The ET2 locus was assigned to rat chromosome 5. The testis-specific histone (HITH) locus also strongly cosegregated with blood pressure in the F2(S X LEW) population and was assigned to rat chromosome 17. Genetic maps of the regions containing the quantitative trait loci (QTL) for blood pressure on chromosomes 5 and 17 were constructed and the QTL were localized using the MAPMAKER/QTL program. The rat genes for endothelin-1, endothelin-3, and endothelin receptor A did not cosegregate with blood pressure in several F2 populations tested and were assigned to rat chromosomes 17, 3, and 19, respectively. Endothelin receptor B cosegregated weakly with blood pressure and was provisionally assigned to rat chromosome 15. We conclude that, in the rat, one new blood pressure QTL is located on chromosome 5 marked by the ET2 locus and another new QTL is located on chromosome 17 near the HITH locus. (J. Clin. Invest. 1994. 93:2701-2709

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Section: Methodsmentioning

confidence: 99%

Genetic mapping of two new blood pressure quantitative trait loci in the rat by genotyping endothelin system genes.

Deng¹,

Dene²,

Pravenec³

et al. 1994

J. Clin. Invest.

121

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show abstract

“…The three genes were mapped in the rat both with a rat-mouse somatic cell hybrid panel (Szpirer et al 1984;Klinga Levan et al 1993) and with FISH. For the mappings with the cell hybrid panel, cDNA probes of approximately 1000 bp from the 3Ј ends of the genes were used (Glul 1111 bp, van de Zande et al 1990; Glud1 957 bp, Das et al 1989;Cps1 883 bp, De Groot et al 1986).…”

mentioning

confidence: 99%

FISH mapping of three ammonia metabolism genes (Glul, Cps1, Glud1) in rat, and the chromosomal localization of GLUL in human and Cps1 in mouse

et al. 1997

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“…It Cell hybrids and Southern blotting. Somatic cell hybrids that segregate rat chromosomes were produced by fusing mouse hepatoma cells (BWTG3) with normal hepatocytes of the Sprague-Dawley rat strain (40). The chromosomal constitution of the hybrid clones is shown in Table 1.…”

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confidence: 99%

Structure and expression of B-myc, a new member of the myc gene family.

et al. 1988

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The myc family of genes contains five functional members. We describe the cloning of a new member of the myc family from rat genomic and cDNA libraries, designated B-myc. A fragment of cloned B-myc was used to map the corresponding rat locus by Southern blotting of DNA prepared from rat x mouse somatic cell hybrids. B-myc mapped to rat chromosome 3. We have previously mapped the c-myc to rat chromosome 7 (J. Sumegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature [London] 306:497-498, 1983) and N-myc and L-myc to rat chromosomes 6 and 5, respectively (S. Ingvarsson, C. Asker, Z. Wirschubsky, J. Szpirer, G. Levan, G. Klein, and J. Sumegi, Somat. Cell Mol. Genet. 13:335-339, 1987). A partial sequence of B-myc had extensive sequence homology to the c-myc protein-coding region, and the detection of intron homology further indicated that these two genes are closely related. The DNA regions conserved among the myc family members, designated myc boxes, were highly conserved between c-myc and B-myc. A lower degree of homology was detected in other parts of the coding region in c-myc and B-myc not present in N-myc and L-myc. A 1.3-kilobase B-myc-specific mRNA was detected in most rat tissues, with the highest expression in the brain. This resembled the expression pattern of c-myc, although at different relative levels, and was in contrast to the more tissue-specific expression of N-myc and L-myc. B-myc was expressed at uniformly high levels in all fetal tissues and during subsequent postnatal development, in contrast to the stage-specific expression of c-myc.

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Gene mapping in the rat by mouse-rat somatic cell hybridization: synteny of the albumin and α-fetoprotein genes and assignment to chromosome 14

Cited by 120 publications

References 13 publications

Genetic mapping of two new blood pressure quantitative trait loci in the rat by genotyping endothelin system genes.

Genetic mapping of two new blood pressure quantitative trait loci in the rat by genotyping endothelin system genes.

FISH mapping of three ammonia metabolism genes (Glul, Cps1, Glud1) in rat, and the chromosomal localization of GLUL in human and Cps1 in mouse

Structure and expression of B-myc, a new member of the myc gene family.

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