Gene mining a marama bean expressed sequence tags (ESTs) database: Embryonic seed development genes and microsatellite marker identification

Sheehama, Emilia N.; Chimwamurombe, Percy

doi:10.5897/ajb2014.14245

Cited by 2 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The availability of EST data in public databases like dbEST facilitates the mining, prediction and characterization of candidate genes by computational biological methods. Several genes having vital functional attributes in processes like seed development [ 12 ], plant growth [ 13 ], and defense response [ 14 , 15 ], microsatellites [ 16 ], and micro-RNAs [ 17 ] have been identified using mining of dbESTs and genome survey sequences (dbGSS) sequences. In the current work, an EST mining-based identification of chitinases in A. cepa has been carried out using already reported plant chitinase sequences as bait.…”

Section: Introductionmentioning

confidence: 99%

In silico analysis of onion chitinases using transcriptome data

Mohapatra

Nanda

2018

Bioinformation

View full text Add to dashboard Cite

Chitinases are glycoside hydrolase (GH) family of proteins having multifaceted roles in plants. It is of interest to identify and characterize chitinase-encoding genes from the popular bulbous plant onion (Allium cepa L.). We have used the EST sequences for onion chitinases to elucidate its functional features using sequence, structure and functional analysis. These contigs belong to the GH19 chitinases family according to domain architecture analysis. They have highly conserved chitinase motifs including motifs exclusive to plant chitinases as implied using the MEME based structural characterization. Estimation of biochemical properties suggested that these proteins have features to form stable and hydrophilic proteins capable of localizing extracellular and in vacuoles. Further, they have multiple cellular processes including defense role as inferred by DeepGO function prediction. Phylogenetic analysis grouped them as class I and class VII plant chitinase, with possible abundance of class I chitinase in onion. These observations help in the isolation and functional validation of onion chitinases.

show abstract

Section: Introductionmentioning

confidence: 99%

In silico analysis of onion chitinases using transcriptome data

Mohapatra

Nanda

2018

Bioinformation

View full text Add to dashboard Cite

show abstract

Systematic Review of the Agro-Ecological, Nutritional, and Medicinal Properties of the Neglected and Underutilized Plant Species Tylosema fassoglense

Munialo,

Gasparatos,

Ludidi

et al. 2024

Sustainability

View full text Add to dashboard Cite

Neglected and Under-utilized plant Species (NUS) can contribute to food system transformation in Sub-Saharan Africa, but many are understudied. Here, we conducted a systematic review of 37 empirical studies to synthesize the evidence from Sub-Saharan Africa about the agro-ecological characteristics, nutritional properties, and medicinal properties of the NUS Tylosema fassoglense. Overall, the evidence is highly fragmented and lacks a comprehensive description of the species characteristics, properties, and benefits. Most reviewed studies focus on the eastern part of Africa and a few on the southern part, suggesting its limited geographic distribution. Studies on agro-ecological properties, and especially on morphological characteristics, indicate that the species’ long tuberous roots and large vegetative systems help it adapt to harsh climatic conditions. The species is also utilized for medicinal and nutrition purposes by different communities across its range due to its phytochemical, macro-, and micronutrient content, along with its favorable physico-chemical properties. In particular, the seeds contain many different amino acids, while their calcium, magnesium, phosphorus, zinc, and carbohydrate content is comparable to Tylosema esculuntum, the better studied species in the Tylosema genus. These demonstrate the species’ potential to contribute to nutrition and food security. Furthermore, parts of the plant contain many phytochemicals, such as phenolics, alkaloids, flavonoid, terpenoids, and tannins (mainly in the roots), and for this reason local communities use it to treat various conditions. Overall, our systematic review suggests that although Tylosema fassoglense has some potential to contribute towards food security, nutrition, health, and livelihoods, there are many remaining knowledge gaps that constrain its effective integration into agricultural production systems and possible contribution to food system transformation in the region.

show abstract

Gene mining a marama bean expressed sequence tags (ESTs) database: Embryonic seed development genes and microsatellite marker identification

Cited by 2 publications

References 13 publications

In silico analysis of onion chitinases using transcriptome data

In silico analysis of onion chitinases using transcriptome data

Systematic Review of the Agro-Ecological, Nutritional, and Medicinal Properties of the Neglected and Underutilized Plant Species Tylosema fassoglense

Contact Info

Product

Resources

About