Gene organization in the UL region and inverted repeats of the canine herpesvirus genome

Abstract

Restriction mapping and the determination of scattered nucleotide sequences have permitted a description of the global structure and evolutionary affinities of the canine herpesvirus (CHV) genome. The global structure closely resembles that of the totally sequenced genomes of varicella-zoster virus and equine herpesvirus 1 (EHV-1) in having a 37 bp inverted repeat flanking a long unique region (UL) of approximately 100000bp, and a 10100-10 700 bp inverted repeat flanking a short unique region (Us) of roughly 7400-8600 bp. On the basis of the sequences obtained, 35 homologues to previously identifled herpesvirus gene products were found in U L and the major inverted repeat, and the level of the similarities indicated that CHV belongs to the genus Varicellovirus. Within the genus, CHV appears to be most closely related to EHV-1, pseudorabies virus and feline herpesvirus. Surprisingly, genes for both subunits of the viral ribonucleotide reductase were found to be missing from their equivalent place in other herpesvirus genomes. Either they have been translocated to another position in the CHV genome or, we think more likely, they have been lost.

Viral molecular and pathological investigations ofCanid herpesvirus1 infection associated respiratory disease and acute death in dogs

“…Taxonomically, CaHV-1 appears to be a monotypic virus that is genetically similar to feline herpesvirus-1 (FHV-1), phocid herpesvirus-1 (PhHV-1) and equine herpesvirus-1 (EHV-1) and -4 (EHV-4) [12,13]. However, the host range of CaHV is restricted to dogs only [14].…”

Molecular characterization of the largest and smallest genome segments, S1 and S12, of Rice gall dwarf virus

“…Cloning and sequencing of CHV-1 a-TIF Remand et al [35] published restriction enzyme maps of CHV-1 genome and 396 bp of sequence for the CHV-1 a-TIF OPF. Their maps and sequence were used to locate a clone from our CHV-1 EcoRI library that contained the complete a-TIF ORF (clone pJT037).…”

Canine respiratory viruses

“…The gene structure of CHV has yet to be determined, since no CHV strain has been completely sequenced and only a few genes have been identified [73,96,132]. Restriction mapping, southern blot hybridization and sequence analysis have shown that the overall structure of CHV resembles those of other alphaherpesviruses and that CHV is genetically related to feline herpesvirus (FHV-1), phocid herpesvirus 1 and to the equid herpesviruses 1 and 4 [103,130,138,169].…”