Gene polymorphisms in PROP1 associated with growth traits in sheep

Ekegbu, Ugonna J.; Burrows, Lucy; Najafabadi, Hamed Amirpour; Zhou, Huitong; Hickford, Jon

doi:10.1016/j.gene.2018.10.024

Cited by 15 publications

(13 citation statements)

References 22 publications

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“…So, some relative genes were detected, the most special gene was LEPR , which not only related to hypoxic, but was also associated with reproductive and fattiness traits in goats and sheep [ 47 , 48 ]. PROP1 (PROP paired-like homeobox 1) ( di value = 15.53), another strongly selected gene, proved to be associated with growth traits of goat and sheep [ 49 , 50 ]. In addition, MKLN1 (muskelin 1) ( di value = 12.37) was associated with backfat thickness in Pigs [ 51 ] and CRH (corticotropin releasing hormone) ( di value = 10.54) proved to be a candidate gene for body conformation traits in cows [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Jin

Lü

Fei

et al. 2020

Animals

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Tibetan goat is an ancient breed, which inhabits the adverse conditions of the plateaus in China. To investigate the role of selection in shaping its genomes, we genotyped Tibetan goats (Nagqu Prefecture, above 4500 m) and three lowland populations (Xinjiang goats, Taihang goats and Huanghuai goats). The result of PCA, neighbor-joining (N-J) tree and model-based clustering showed that the genetic structure between the Tibetan goat and the three lowland populations has significant difference. As demonstrated by the di statistic, we found that some genes were related to the high-altitude adaptation of Tibetan goats. Functional analysis revealed that these genes were enriched in the VEGF (vascular endothelial growth factor) signaling pathway and melanoma, suggesting that nine genes (FGF2, EGFR, AKT1, PTEN, MITF, ENPEP, SIRT6, KDR, and CDC42) might have important roles in the high-altitude adaptation of Nagqu Tibetan goats. We also found that the LEPR gene was under the strongest selection (di value = 16.70), and it could induce upregulation of the hypoxic ventilatory response. In addition, five genes (LEPR, LDB1, EGFR, NOX4 and FGF2) with high di values were analyzed using q-PCR. Among them, we found that LEPR, LDB1 and FGF2 exhibited higher expression in the lungs of the Tibetan goats; LEPR, EGFR and LDB1 exhibited higher expression in the hearts of the Huanghuai goat. Our results suggest that LEPR, LDB1, EGFR and FGF2 genes may be related to the high-altitude adaptation of the goats. These findings improve our understanding of the selection of the high-altitude adaptability of the Nagqu Tibetan goats and provide new theoretical knowledge for the conservation and utilization of germplasm resources.

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Section: Resultsmentioning

confidence: 99%

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Jin

Lü

Fei

et al. 2020

Animals

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“…To our current knowledge, apart from the short communication of Sanz et al [155] that identified novel polymorphisms in the 5 UTR of FASN, PROP1, GPAM, MC4R, FADS and PLIN1 ovine candidate genes and their relationships with gene expression and diet in a study with Spanish sheep (Rasa Aragonesa, Roja Mallorquina and Assaf), Chinese Sunit sheep [156], and New Zealand sheep [157], there is no literature published on FASN-FADS-PROP1 genes and their correlations with growth and meat quality traits in TAW or any other sheep breed, thus presenting a significant knowledge gap.…”

Section: Fatty Acid Synthase (Fasn)mentioning

confidence: 90%

“…Therefore, FABP4 polymorphisms are believed to have a significant influence on live performance and carcass characteristics [162,171], meat tenderness, marbling score and IMF content in sheep. For instance, in Romney sheep, [156,157] reported five variants (A1 − E1) in region-1 (exon 2 − intron 2) and three variants (A2 − C2) in region-2 (exon 3 − intron 3) wherein A1 was associated with a decrease in leg, loin and total meat yield, while A2 was associated with a decrease in weaning weight and pre-weaning growth rate. Haplotype A1-A2 was found to be associated with a decrease in birth weight, pre-weaning growth-rate, hot carcass weight, loin meat yield, shoulder meat yield and total meat yield, while haplotype A1-B2 was associated with increased fat depth at the 12th rib (V-GR).…”

Section: Fatty Acid Binding Protein4 (Fabp4)mentioning

confidence: 99%

Genetics of Omega-3 Long-Chain Polyunsaturated Fatty Acid Metabolism and Meat Eating Quality in Tattykeel Australian White Lambs

Pewan

Otto

Huerlimann

et al. 2020

Genes

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Meat eating quality with a healthy composition hinges on intramuscular fat (IMF), fat melting point (FMP), tenderness, juiciness, flavour and omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) content. These health-beneficial n-3 LC-PUFA play significant roles in optimal cardiovascular, retinal, maternal and childhood brain functions, and include alpha linolenic (ALA), eicosapentaenoic (EPA), docosahexaenoic (DHA) and docosapentaenoic (DPA) acids. The primary objective of this review was to access, retrieve, synthesise and critically appraise the published literature on the synthesis, metabolism and genetics of n-3 LC-PUFA and meat eating quality. Studies on IMF content, FMP and fatty acid composition were reviewed to identify knowledge gaps that can inform future research with Tattykeel Australian White (TAW) lambs. The TAW is a new sheep breed exclusive to MARGRA brand of lamb with an outstanding low fat melting point (28–39°C), high n-3 LC-PUFA EPA+DHA content (33–69mg/100g), marbling (3.4–8.2%), tenderness (20.0–38.5N) and overall consumer liking (7.9–8.5). However, correlations between n-3 LC-PUFA profile, stearoyl-CoA desaturase (SCD), fatty acid binding protein 4 (FABP4), fatty acid synthase (FASN), other lipogenic genes and meat quality traits present major knowledge gaps. The review also identified research opportunities in nutrition–genetics interactions aimed at a greater understanding of the genetics of n-3 LC-PUFA, feedlot finishing performance, carcass traits and eating quality in the TAW sheep. It was concluded that studies on IMF, FMP and n-3 LC-PUFA profiles in parental and progeny generations of TAW sheep will be foundational for the genetic selection of healthy lamb eating qualities and provide useful insights into their correlations with SCD, FASN and FABP4 genes.

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“…Researchers have previously given considerable attention to the analysis of molecular markers associated with economic traits in a number of species. For example, genetic variation in the ovine PROP1 gene was identified in 670 New Zealand Romney sheep, which were related to traits such as tailing weight and growth rate [38]. One 43-bp indel polymorphism in HS6ST3 was associated with shank length, chest depth and other growth traits in 860 chickens [39].…”

Section: Discussionmentioning

confidence: 99%

Copy Number Variation of the CADM2 Gene and Its Association with Growth Traits in Yak

Chen

et al. 2019

Animals

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Simple Summary: Cell adhesion molecule 2 (CADM2), also known as synaptic cell adhesion molecule 2 (SYNCAM2), is the mediator of synaptic signals enriched in the brain. Overlaps between copy number variation (CNV) regions in CADM2 and quantitative trait loci (QTL) related to body weight have been clarified in a previous study. In this study, two loci were amplified in the CADM2 gene (CNV1: 235,915 bp, exon 1 and partial intron 1; CNV2: 60,430 bp, intron 9) to explore the relationship between CNV types in the CADM2 gene and growth traits in 350 Ashidan yaks. Association analysis illustrated that no significant effect was found on growth traits in CNV1. However, the CNV2 mutation had a significant effect on body weight at the sixth month (p < 0.05). Individuals with the gain-type copy number variation CNV2 were significantly superior to those with loss-or normal-type in terms of body weight (p < 0.05). In summary, this study confirmed that CADM2-CNVs affect growth traits in yaks, and may be candidate genes for successful yak breeding and genetics projects.Abstract: Copy number variation (CNV) is currently accepted as a common source of genetic variation. It is reported that CNVs may influence the resistance to disease and complex economic traits, such as residual feed intake, muscle formation, and fat deposition in livestock. Cell adhesion molecule 2 (CADM2) is expressed widely in the brain and adipose tissue and can regulate body weight through the central nervous system. Growth traits are important economic traits for animal selection. In this study, we aimed to explore the effect of CADM2 gene copy number variants on yak growth traits. Here, two CNVs in the CADM2 gene were investigated using the quantitative polymerase chain reaction (qPCR), and the association of the CNVs with growth traits in yak was analyzed using statistical methods by SPSS software. Differences were considered significant if the p value was < 0.05. Statistical analysis indicated significant association of CADM2-CNV2 with the body weight of the Chinese Ashidan yak. A significant effect of CNV2 (p < 0.05) was found on body weight at 6 months. In CNV2, the gain-type copy number variation exhibited greater performance than the other variants, with greater body weight observed at 6 months (p < 0.05). To the best of our knowledge, this is the first attempt to investigate the function of CADM2-CNVs and their association with growth traits in animals. This may be a useful candidate marker in marker-assisted selection of yaks.Animals 2019, 9, 1008 2 of 11 polymorphisms (RFLP), single nucleotide polymorphisms (SNP), indels (Insertion/Deletion) and structural variations, such as inversions, duplications and deletions. To date, SNPs are used extensively for complex economic disease traits in livestock [2][3][4][5]. More than 99% of indels are less than 50 bp in length [6,7], and some long insertions and deletions are returned into the concept of structural variation [8]. As a genetic marker, indels have great potential whether in growth and reproduction...

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Gene polymorphisms in PROP1 associated with growth traits in sheep

Cited by 15 publications

References 22 publications

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Genetics of Omega-3 Long-Chain Polyunsaturated Fatty Acid Metabolism and Meat Eating Quality in Tattykeel Australian White Lambs

Copy Number Variation of the CADM2 Gene and Its Association with Growth Traits in Yak

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