Gene segregation in the two-spored basidiomycete Coprinus bilanatus

Kemp, R.F.O.

doi:10.1038/hdy.1985.56

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…Coprinus bilanatus), a bisporic bifactorial (tetrapolar) species (Kemp 1974) with two mating type loci tightly linked to their respective centromeres (Elliott and Challen 1983;Kemp 1985). For this species, Challen and Elliott (1989) proposed a random bisporic model with a category of heterokaryotic spores called Bsister nuclei^progeny because they receive nuclei arising from the same second meiotic division and bearing the same mating type alleles.…”

Section: Introductionmentioning

confidence: 99%

Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens

Brito

Foulongne-Oriol

Moinard

et al. 2015

Appl Microbiol Biotechnol

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Strain breeding is much less advanced in the edible and medicinal species Agaricus subrufescens than in Agaricus bisporus, the button mushroom. Both species have a unifactorial system of sexual incompatibility, a mating type locus tightly linked to a centromere, and basidia producing both homokaryotic (n) and heterokaryotic (n + n) spores. In A. bisporus, breeding is mainly based on direct selection among the heterokaryotic offspring and on hybridization between homokaryotic offspring. The parental heterozygosity is highly maintained in the heterokaryotic offspring due to suppression of recombination and preferential pairing in the spores of nuclei, each one per second meiotic divisions; such "non-sister nuclei" heterokaryons are fertile. In A. subrufescens, recent studies revealed that recombination is not suppressed and that nuclei from the same second meiotic division can also be paired in a spore that give rise to a "sister nuclei" heterokaryon in which the nuclei bear the same mating type allele. The objective of the present work was to investigate the potential function of the different categories of spores in A. subrufescens and their possible use in a genetic breeding program. Using eight co-dominant molecular markers, we found that half of the offspring of the A. subrufescens strain WC837 were heterokaryotic, one quarter of them being sister nuclei heterokaryons. These heterokaryons were infertile and behaved like homokaryons, being even able to cross between each other. In contrast, non-sister nuclei heterokaryons could fruit but inconsistently due to inbreeding depression. Potential roles of these two categories of heterokaryons in nature and consequences for strain breeding are discussed.

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Section: Introductionmentioning

confidence: 99%

Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens

Brito

Foulongne-Oriol

Moinard

et al. 2015

Appl Microbiol Biotechnol

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Segregation of genetic markers in the two-spored secondarily homothallic basidiomycete Coprinus bilanatus

Challen¹,

Elliott²

1989

Theoret. Appl. Genetics

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Secondarily homothallic basidiomycetes, of which the cultivated mushroom Agaricus bisporus is an example, produce both self-fertile and non self-fertile spores. The random migration of nuclei from the basidia to give binucleate spores provides the simplest explanation for the regulation of breeding behaviour in this group of fungi. To test the predictions of the random migration hypothesis, the segregation of mating-type, auxotrophy and antimetabolite resistance has been determined in the secondarily homothallic ink-cap fungus, Coprinus bilanatus. In 41 of a total of 56 spore progenies tested, the segregation ratios conformed to the predictions of the random migration hypothesis. Poor fits to the predicted ratios were, in many instances, associated with an adenine auxotrophy. On the basis of the data reported, random migration can be regarded as the primary control of secondary homothallism.

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Nuclear Behavior in the Basidia of the Secondarily HomothallicCoprinus Bilanatus

Ross

Margalith

1987

Mycologia

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Gene segregation in the two-spored basidiomycete Coprinus bilanatus

Cited by 3 publications

References 8 publications

Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens

Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens

Segregation of genetic markers in the two-spored secondarily homothallic basidiomycete Coprinus bilanatus

Nuclear Behavior in the Basidia of the Secondarily HomothallicCoprinus Bilanatus

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