Gene Silencing Using Artificial miRNA in Sugarcane

Peres, Ana Laura Garcia Leme; Coletta, Rafael Della; Soares, José Sérgio; Menossi, Marcelo

doi:10.1007/s12042-022-09313-x

Cited by 3 publications

(3 citation statements)

References 35 publications

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“…As obvious, control plants did not exhibit any trace of TH due to the absence of ThSyGII. Although, post transcriptional gene silencing (PTGS) is very common issue in sugarcanedue to complexity of its genome poses serious challenges for transgene stability 31 . Different SIGs contain antagonistic mechanism resisting PTGS.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Awan

Ali

Iqbal

et al. 2022

Sci Rep

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Enhancement of sugar contents and yielding healthful sugar products from sugarcane demand high profile scientific strategies. Previous efforts to foster manipulation in metabolic pathways or triggering sugar production through combating abiotic stresses fail to yield high sugar recovery in Saccharum officinarum L. Novel sucrose isomers trehalulose (TH) and isomaltulose (IM) are naturally manufactured in microbial sources. In pursuance of novel scientific methodology, codon optimized sucrose isomerase gene, Trehalulose synthase gene II(CEMB-SIG2) cloned under dual combined stem specific constitutive promoters in pCAMBIA1301 expression vector integrated with Vacuole targeted signal peptide (VTS) to concentrate gene product into the vacuole. The resultant mRNA expression obtained by Real Time PCR validated extremely increased transgene expression in sugarcane culms than leaf tissues. Overall sugar estimation from transgenic sugarcane lines was executed through refractometer. HPLC based quantifications of Trehalulose (TH) alongside different internodes of transgenic sugarcane confirmed the enhancement of boosted sugar concentrations in mature sugarcane culms. Trehalulose synthase gene II receptive sugarcane lines indicated the unprecedented impressions of duly combined constitutive stem regulated promoters. Transgenic sugarcane lines produce highest sugar recovery percentages, 14.9% as compared to control lines (8.5%). The increased sugar recovery percentage in transgenic sugarcane validated the utmost performance and expression of ThSyGII gene .High Profile Liquid chromatography based sugar contents estimation of Trehalulose (TH) and Isomaltulose (IM) yielded unprecedented improvement in the whole sugar recovery percentage as compared to control lines.⁠

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Section: Discussionmentioning

confidence: 99%

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Awan

Ali

Iqbal

et al. 2022

Sci Rep

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“…Один вариант микроРНК может взаимодействовать с несколькими частично комплементарными им мРНК и таким образом регулировать экспрессию контролирующих их генов (Schvab et al, 2006). В свою очередь искуственные микроРНК позволяют повысить специфичность и ограничить нецелевые эффекты РНКи (Schvab et al, 2006;Peres et al, 2022).…”

Section: нецелевое действие рнки и пути его предотвращенияunclassified

Untitled

2023

1727-1320

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The predatory bug Podisus maculiventris is a polyphagous entomophagous insect, considered as a promising agent of biological protection of potato against the Colorado potato beetle in Western Siberia. In order to reduce the economic costs of its transportation and mass production, it is advisable to improve and adapt the technology of its breeding and application to the conditions of the region. Under laboratory conditions, the effectiveness of different prey species (larvae of the greater wax moth, diamondback moth, yellow mealworm beetle and vetch aphid) to maintain high indicators of the stock culture of the predatory bug was revealed. With a mixed diet, a high survival rate of entomophagous larvae was observed, the number of adults of the next generation averaged 78 % of the initial number, the weight of females and males increased to 75 and 56 mg, respectively, and fertility reached 540 eggs per female. In laboratory conditions, the voracity of the predator varied from 1.8 to 8.4 individuals of the Colorado potato beetle, depending on the stage of development of the entomophagous agent and the age of the pest larvae. As a result of three-year field trials (2018-2020) on potatoes of the Yuna variety, the biological effectiveness of the predator against the Colorado potato beetle ranged from 66 to 85 %, and with the combined use of the predatory bug and the biological preparation Bitoksibatsillin increased to 98.5 %. In 2020, the efficiency of the entomophagous agent released at the predator-prey ratio of 1: 5.0-6.5 ranged between 63 and 75 % on different varieties of potato.

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Section: нецелевое действие рнки и пути его предотвращенияunclassified

RNA interference in the study of gene functions in aphids and other hemipterans and the fight against their harmful representatives

Konarev

2023

1727-1320

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RNA interference (RNAi) is an important mechanism that protects cells of eukaryotic organisms from foreign genetic information and regulates physiological processes. Discovery of RNAi and development of the methods for targeted suppression of the expression of individual genes have opened up wide opportunities for elucidating gene functions and solving many problems in biology, medicine, and plant protection. This approach can contribute to the development of new effective, highly specific, and environmentally safe ways to control harmful insects. Several examples of the practical use of RNAi for combating chewing pests have been already reported. Due to their biology, phloem-feeding, as well as the ways of delivering genetic constructs into the body, RNAi in insects with piercing-sucking mouthparts – aphids, bugs, and other hemipterans – deserves separate consideration. This review discusses the criteria for selecting target genes and the issues of their functional activity solved by RNAi, methods for delivering double-stranded and small RNAs to organisms of various Hemiptera, the non-target effects of RNAi and the ways to prevent them, as well as possible practical outcomes of using this approach to control hemipteran pests of agricultural plants and vectors of dangerous human and animal diseases.

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Gene Silencing Using Artificial miRNA in Sugarcane

Cited by 3 publications

References 35 publications

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

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RNA interference in the study of gene functions in aphids and other hemipterans and the fight against their harmful representatives

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