Gene Specific DNA Sensors for Diagnosis of Pathogenic Infections

Abstract

Gene specific DNA based sensors have potential applications for rapid and real time monitoring of hybridization signal with the target nucleic acid of pathogens. Different types of DNA based sensors and their applications have been studied for rapid and accurate detection of pathogens causing human diseases. These sensors are based on surface plasmon resonance, quantum-dots, molecular beacons, piezoelectric and electrochemical etc. Curbing epidemics at an early stage is one of the massive challenges in healthcare systems. Timely detection of the causative organism may provide a solution to restrain mortality caused by the disease. With the advent of interdisciplinary sciences, bioelectronics has emerged as an effective alternative for disease diagnostics. Gene specific DNA sensors present themselves as cost-effective, sensitive and specific platforms for detection of disease causing pathogens. The mini review explores different transducer based sensors and their potential in diagnosis of acute and chronic diseases.

Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications

“…They were able to prove the target DNA with limit of detection 10 −19 mol/L in urine samples during a half hour period. The piezoelectric immunosensors with embedded genetic information are a good tool for the identification of pathogenic microorganisms [ 55 ]. The idea of a DNA-detecting biosensor was adopted in some works.…”

Emerging Designs of Electronic Devices in Biomedicine

“…Concerning other experimental techniques in infection diagnostics and specific biosensor approaches, refer to the work by Datta et al [152].…”

Recent advances and challenges in electrochemical biosensors for emerging and re-emerging infectious diseases

“…The advancements made in emerging techniques like nanotechnology and microfluidics, coupled with the identification of biomarkers would boost the efficiency of healthcare sector. Transducer integration in biomaterials has allowed for the development of interfaces capable of producing signals - aptly discussed as biosensors [ 10 ]. Biosensors, which are bio-electromechanical systems, can be classified based on transducer form, label and general configuration [ 10 ].…”

“…Transducer integration in biomaterials has allowed for the development of interfaces capable of producing signals - aptly discussed as biosensors [ 10 ]. Biosensors, which are bio-electromechanical systems, can be classified based on transducer form, label and general configuration [ 10 ]. Biosensors are designed to suit specific functionalities and affinities.…”

“…Biosensors are designed to suit specific functionalities and affinities. Recognition of analytes can often contribute to a transition in three-dimensional structure that is further transduced as a signal [ 10 ].…”