Gene Transfer Techniques and their relevance to Woody Plants

Minocha, Subhash C.; Wallace, John C.

doi:10.1007/978-94-017-2313-8_1

Cited by 2 publications

(1 citation statement)

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“…Parameters such as vector constructions, transformation methods, transgene integration, and inheritance of transgene need to be carefully considered to ensure the efficiency of the successful transformation event (Lee-Yoon Low et al 2018 ). This has also been explored in prior studies; gene transfer in the plant can be categorized into two methods: vector-mediated gene transfer and direct gene transfer through physical or chemical reactions (Minocha and Wallace 2000 ). Some species are amenable to Agrobacterium -mediated transformation of flowers, allowing progeny derived from sexual propagation to be screened for transformants and edits.…”

Section: Introductionmentioning

confidence: 99%

Current status and prospects of plant genome editing in Australia

Zhang

Restall

Crisp

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Plant genome editing, particularly CRISPR-Cas biotechnologies, has rapidly evolved and drawn enormous attention all around the world in the last decade. The cutting-edge technologies have had substantial impact on precise genome editing for manipulating gene expression, stacking gene mutations, and improving crop agronomic traits. Following the global trends, investigations on CRISPR-Cas have been thriving in Australia, especially in agriculture sciences. Importantly, CRISPR-edited plants, classified as SDN-1 organisms (SDN: site-directed nuclease), have been given a green light in Australia, with regulatory bodies indicating they will not be classified as a genetically modified organism (GMO) if no foreign DNA is present in an edited plant. As a result, genome-edited products would not attract the onerous regulation required for the introduction of a GMO, which could mean more rapid deployment of new varieties and products that could be traded freely in Australia, and potentially to export markets. In the present review, we discuss the current status and prospects of plant genome editing in Australia by highlighting several species of interest. Using these species as case studies, we discuss the priorities and potential of plant genome editing, as well as the remaining challenges.

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Section: Introductionmentioning

confidence: 99%