Gene transfer vectors derived from equine infectious anemia virus

Abstract

Equine infectious anemia virus (EIAV) is a lentivirus in the genes into cultured human cells. In addition, stable helper retrovirus family of viruses. Replication-defective EIAV veccell lines were created by modifying human 293 cells to tors have been constructed that encode bacterial puromyexpress EIAV proteins. Unlike retroviral vectors based on cin-N-acetyl transferase and E. coli ␤-galactosidase. These murine leukemia virus, EIAV lentiviral vectors transduce vectors could be prepared with titers greater than 10 5 infecnondividing (aphidicolin-arrested) cells. These properties tious units/ml and were able to act as vehicles to carry make EIAV vectors promising gene transfer vehicles.

Highly efficient EIAV-mediated in utero gene transfer and expression in the major muscle groups affected by Duchenne muscular dystrophy

“…19,20 Cells were grown in DMEM supplemented with 10% fetal calf serum and transfected with plasmid vectors to transiently produce infectious virus particles as previously described. 21 Virus titration was performed on D17 canine cells and human HT1080 cells by seeding cells onto 12-well culture dishes at 8 Â 10 4 cells/well followed by the addition of serially diluted virus. Virus was left on cells for 24 h before removal.…”

Long-term transgene expression by administration of a lentivirus-based vector to the fetal circulation of immuno-competent mice

“…22,37 SMART2Z is an EIAV vector expressing the b-galactosidase gene driven by the CMV promoter. It also contains the central polypurine tract from EIAV (cppt) 38 and the WPRE element from the woodchuck hepatitis virus.…”

“…In particular, lentiviruses that can infect nondividing cells 18 have been shown to provide more efficient gene transfer and sustained gene expression in vivo. 20,21 MLV-based vectors have been applied by intratracheal, intrahepatic, and intraperitoneal injection to fetal sheep and rats; 18,[22][23][24][25] however, only low levels of transgene expression were observed in all cases. Intraperitoneal vector injection to fetal sheep at 60 days of gestation resulting in marker gene expression in haematopoietic cells until at least 40 months was attributed both to the expression from an integrating vector system as well as to the induction of immune-tolerance against the transgenic protein.…”

Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

“…The extensive body of knowledge that has been generated on this virus has allowed researchers to reengineer it as a gene vector. HIV-1 remains the best studied vector (Lever et al 2004), although recent work has revealed the potential of HIV-2 (Sadaie et al 1998) SIV (Schnell et al 2000;Stitz et al 2001), EIAV (equine infectious anemia virus) (Olsen 1998;Mitrophanous et al 1999;Ikeda et al 2003) 2002; Loewen et al 2003) and BIV (bovine immunodeficiency virus) (Takahashi et al 2002) as vectors.…”