General considerations regarding the thin slice plastination technique

Sora, Mircea‐Constantin; Matusz, Petru

doi:10.1002/ca.21013

Cited by 13 publications

(15 citation statements)

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“…Gunther von Hagens invented plastination in 1978 in Heidelberg, Germany. It consists of the forced impregnation, in a vacuum, of biological specimens with reactive polymers (Marks et al, ; Sora and Matusz, ; Ottone et al, ). The technique for sheet plastination to produce transparent slices in E12 epoxy resins was patented in 1982 (U.S. Patent 4,320,157) (Bickley and Townsend, ).…”

Section: Introductionmentioning

confidence: 99%

“…Plastination of biological tissues with E12 epoxy resin allows thin sections of tissue to be preserved for anatomical study (Reed, ; Borzooeian and Enteshari, ; Sora and Cook, ; Sora and Matusz, ). During the E12 plastination technique, the water and lipids in the biological tissues are replaced with curable epoxy.…”

Section: Introductionmentioning

confidence: 99%

“…In general, 3–5 mm sections are used for descriptive and morphometric analysis, and ultra‐thin sections (<1 mm) for three‐dimensional reconstruction or histological examination (Cook, ; Gruber et al, ; Sora et al, , ). It is the preferred technique for producing transparent body sections because shrinkage is minimal compared to the sheet plastination technique using polyester (Bickley and Townsend, ; Sora et al, ; Sora and Matusz, ). Polyester plastination (P40 plastination technique) was designed to preserve 3–5 mm thick brain sections, enhancing the distinction between gray and white matter, but it can also be used for other anatomical regions.…”

Section: Introductionmentioning

confidence: 99%

“…Transparent sections of anatomical regions allow the topography of all body structures to be studied for teaching and research purposes in an uncollapsed state and without displacement (Cook, ; Skalkos et al, ; Sora et al ; Sora and Cook, ; Soal et al ; Sora and Matusz, ; Kürtül et al, ; Ottone et al, ). They preserve the anatomical positions of all the tissues in situ and can be examined at macro‐ and micro‐scopic levels (Cook and Al‐Ali, ; Leaper et al, ; Thorpe Lowis et al, ).…”

Section: Introductionmentioning

confidence: 99%

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E12 sheet plastination: Techniques and applications

et al. 2017

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Plastination is an anatomical technique that consists of replacing the liquids and fat of specimens by reactive polymers through forced impregnation in a vacuum. These are then polymerized to achieve the final result. E12 sheet plastination involves epoxy resin impregnation of thin (2-4 mm) and ultra-thin (<2 mm) tissue sheets, producing dry, transparent, odorless, non-toxic and long-lasting sheets. E12 sheet plastination techniques were reviewed using MEDLINE, EMBASE and SciELO databases, and manual searches. After searching, 616 records were found using the online and manual searches (MEDLINE, n: 207; EMBASE, n: 346; SciELO, n: 44; Manual search: 23). Finally, 96 records were included in this review (after duplicates and articles unrelated to the subject were excluded). The aim of this work was to review the E12 sheet plastination technique, searching for articles concerning views of it, identifying the different variants implemented by researchers since its creation by Gunther von Hagens, and to identify its applications from teaching and research in anatomy to morphological sciences. Clin. Anat. 31:742-756, 2018. © 2017 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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E12 sheet plastination: Techniques and applications

et al. 2017

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“…Sora and Matusz (2010) who have themselves made significant contributions to the field of sheet plastination. We are pleased that they view our study as an important step in finding alternatives to classic thin slice plastination.…”

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Visualization of the dentogingival junction using micro‐plastination technique

Correa‐Aravena,

Panes,

Ponce

et al. 2024

Clinical Anatomy

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Plastination has revolutionized the field of anatomy and research by providing biosecurity and enabling the long‐term preservation of biological material, ranging from entire bodies to individual organs and even micron sections. The dentogingival junction (DGJ) consists of both epithelial and connective tissues that are closely related to the tooth's mineralized tissues. Cutting‐grinding techniques are commonly used to visualize DGJ histology. These techniques exclude enamel from preparations and focus on visualizing hard or soft tissues. To improve the micro‐anatomical and histological study of this region, we applied micro‐plastination technique to obtain micro‐thin slices below 150 μm thick from human and animal samples. The DGJ microanatomy was visualized by applying histological stains to the micro‐plastinated slices, highlighting the technique's endogenous autofluorescence capacity identifying periodontal tissues, including dentin, enamel, cementoenamel junction, dentinal tubules, connective tissue, and collagen. Based on our results, we confirm that micro‐plastination is a useful technique for visualizing anatomical regions that are difficult to access, such as the DGJ. Micro‐plastination can be used as an alternative technique, providing a new approach for its application in anatomical and morphological research protocols.

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General considerations regarding the thin slice plastination technique

Cited by 13 publications

References 14 publications

E12 sheet plastination: Techniques and applications

E12 sheet plastination: Techniques and applications

Reply: General considerations regarding the thin slice plastination technique

Visualization of the dentogingival junction using micro‐plastination technique

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