Generation and Field Trials of Transgenic Rice Tolerant to Iron Deficiency

Kobayashi, Takanori; Nakanishi, Hiromi; Takahashi, Michiko; Mori, Satoshi; Nishizawa, Naoko K.

doi:10.1007/s12284-008-9011-x

Cited by 25 publications

(14 citation statements)

References 72 publications

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“…Iron deficiency is a major cause of reduced crop yields, particularly in calcareous soils, which account for about 30% of the world's cultivated soils (Kobayashi et al, 2008). When barley nicotianamine aminotransferase genes are expressed in rice, the transformants show enhanced tolerance to low Fe-availability (Takahashi et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Activation of Rice nicotianamine synthase 2 (OsNAS2) Enhances Iron Availability for Biofortification

Lee

Kim

Jeon

et al. 2012

Molecules and Cells

123

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Section: Discussionmentioning

confidence: 99%

Activation of Rice nicotianamine synthase 2 (OsNAS2) Enhances Iron Availability for Biofortification

Lee

Kim

Jeon

et al. 2012

Molecules and Cells

123

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“…Furthermore, individual event‐based analysis revealed that all transformation methods could produce events with yield equivalent to the nontransformed PC albeit at varying efficiencies. Yield trials in barley (Kobayashi et al ., ), wheat (Shewry et al ., ), and rice (Travella et al ., ) also concluded that equivalence in transgenic plants was achievable. In field studies of transgenic potato (which, like sugarcane, also has a highly heterozygous genome), the authors concluded that although higher somaclonal variation existed in transgenic plants, many of them showed equivalence to PC, thus enabling selection of plants with desirable characteristics with no yield penalty (Dale and McPartlan, ).…”

Section: Discussionmentioning

confidence: 99%

Field performance of transgenic sugarcane produced using Agrobacterium and biolistics methods

Joyce

Hermann

O’Connell

et al. 2013

Plant Biotechnology Journal

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SummaryFuture genetic improvement of sugarcane depends, in part, on the ability to produce highyielding transgenic cultivars with improved traits such as herbicide and insect resistance. Here, transgenic sugarcane plants generated by different transformation methods were assessed for field performance over 3 years. Agrobacterium-mediated (Agro) transgenic events (35) were produced using four different Agrobacterium tumefaciens strains, while biolistic (Biol) transgenic events (48) were produced using either minimal linearized DNA (LDNA) transgene cassettes with 5′, 3′ or blunt ends or whole circular plasmid (PDNA) vectors containing the same transgenes. A combined analysis showed a reduction in growth and cane yield in Biol, Agro as well as untransformed tissue culture (TC) events, compared with the parent clone (PC) Q117 (no transformation or tissue culture) in the plant, first ratoon and second ratoon crops. However, when individual events were analysed separately, yields of some transgenic events from both Agro and Biol were comparable to PC, suggesting that either transformation method can produce commercially suitable clones. Interestingly, a greater percentage of Biol transformants were similar to PC for growth and yield than Agro clones. Crop ratoonability and sugar yield components (Brix%, Pol%, and commercial cane sugar (CCS)) were unaffected by transformation or tissue culture. Transgene expression remained stable over different crop cycles and increased with plant maturity. Transgene copy number did not influence transgene expression, and both transformation methods produced low transgene copy number events. No consistent pattern of genetic changes was detected in the test population using three DNA fingerprinting techniques.

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“…The combined expression of ferritin and phytase had been achieved in rice and maize (Table 4) thus increasing Fe levels and availability in simulated digestion/absorption trials (Lucca et al 2002;Drakakaki et al 2005). As an unexpected bonus, the transgenic plants with enhanced Fe accumulation are also more tolerant of Fe-depleted and high-pH soils (Takahashi et al 2001;Takahashi 2003;Kobayashi et al 2008).…”

Section: Ironmentioning

confidence: 99%

Critical evaluation of strategies for mineral fortification of staple food crops

et al. 2009

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Staple food crops, in particular cereal grains, are poor sources of key mineral nutrients. As a result, the world's poorest people, generally those subsisting on a monotonous cereal diet, are also those most vulnerable to mineral deficiency diseases. Various strategies have been proposed to deal with micronutrient deficiencies including the provision of mineral supplements, the fortification of processed food, the biofortification of crop plants at source with mineral-rich fertilizers and the implementation of breeding programs and genetic engineering approaches to generate mineral-rich varieties of staple crops. This review provides a critical comparison of the strategies that have been developed to address deficiencies in five key mineral nutrients-iodine, iron, zinc, calcium and selenium-and discusses the most recent advances in genetic engineering to increase mineral levels and bioavailability in our most important staple food crops.

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Generation and Field Trials of Transgenic Rice Tolerant to Iron Deficiency

Abstract: Iron

Cited by 25 publications

References 72 publications

Activation of Rice nicotianamine synthase 2 (OsNAS2) Enhances Iron Availability for Biofortification

Activation of Rice nicotianamine synthase 2 (OsNAS2) Enhances Iron Availability for Biofortification

Field performance of transgenic sugarcane produced using Agrobacterium and biolistics methods

Critical evaluation of strategies for mineral fortification of staple food crops

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