Generation and molecular marker and cytological characterization of wheat – Secale strictum subsp. anatolicum derivatives

Abstract

Cereal rye and its wild forms are important sources of genetic diversity for wheat breeding due to their resistances to biotic and abiotic stresses. <i>Secale strictum</i> subsp. <i>anatolicum </i>(Boiss.) K.Hammer (SSA) is a weedy relative of cultivated rye, <i>S. cereale</i>. Meiotic chromosome pairing in F<sub>1</sub> hybrids of SSA and <i>S. cereale</i> reveals strong genomic affinity between the two genomes. A study of the transferability of <i>S. cereale</i> sequence-based markers to SSA and hexaploid triticale demonstrated their applicability for tracing SSA chromatin in wheat. The transferability of the markers was over 80% from homoeologous groups 1, 2 and 3, and greater than 70% from groups 4 to 7, respectively. This study focused on the generation and molecular and cytogenetic characterization of wheat-SSA alien derivatives. Twelve were identified using combinations of non-denaturing fluorescence in situ hybridization (ND-FISH), genomic in situ hybridization (GISH) and molecular marker analysis. All SSA chromosomes, except 3R<sup>a</sup> and 6R<sup>a</sup>, were transferred to wheat either in the form of monosomic (MAs), mono-telosomic (MtAs), double-mono-telosomic (dMtAs) or double-monosomic (dMAs) additions. The germplasm developed in this study will help to enhance the genetic base of wheat and facilitate molecular breeding of wheat and triticale.

Aegilops crassa Boiss. repeatome characterized using low-coverage NGS as a source of new FISH markers: Application in phylogenetic studies of the Triticeae

“…Development of new markers for genome studies is an important task in molecular biology and cytogenetics ( Du et al, 2017 ; Meng et al, 2018 ; Said et al, 2018 ; Liu et al, 2019 ; Nikitina et al, 2020 ; Xi et al, 2020 ; Zagorski et al, 2020 ; Li et al, 2021 ; Liu and Zhang, 2021 ; Singh et al, 2021 ). Current progress in plant genome sequencing and bioinformatic analysis has opened broad perspectives for discovering new DNA sequences that can potentially be used as FISH probes, and the number of such markers rapidly increases.…”

Fine mapping of QYrsv.swust-1BL for resistance to stripe rust in durum wheat Svevo

“…At present, 86 formally named resistance genes, more than 70 temporarily named genes, and more than 380 quantitative trait loci (QTL) for resistance to stripe rust have been identified from common wheat, durum wheat, and wild relatives ( Bansal et al., 2014 ; Wang and Chen, 2017 ; Feng et al., 2018 ; Nsabiyera et al., 2018 ; Gessese et al., 2019 ; Maccaferri et al., 2019 ; Pakeerathan et al., 2019 ; Li et al., 2020 ; Singh et al., 2021 ; Klymiuk et al., 2022 ; Yao et al, 2022 ; Feng et al., 2023 ; Zhu et al., 2023 ). However, most of the genes were overcome by the pathogen shortly after being introduced to commercial cultivars.…”

The whole chloroplast genome of Secale strictum subsp. kuprijanovii, a wild and perennial kinship to cultivated rye

“…Cereal rye and its wild forms provide important genetic resources for wheat breeding due to their excellent resistance to different stress (Singh et al. 2021 ). Secale strictum subsp.…”