Generation, annotation and analysis of ESTs from Trichoderma harzianum CECT 2413

Vizcaíno, Juan Antonio; González, Francisco; Suárez, M. Belén; Redondo, José; Heinrich, Julian; Delgado-Jarana, Jesús; Hermosa, Rosa; Gutiérrez, Santiago; Monte, Enrique; Llobell, Antonio; Rey, Manuel

doi:10.1186/1471-2164-7-193

Cited by 62 publications

(22 citation statements)

References 59 publications

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“…Starting in 2002, the TrichoEST functional genomics project sequenced more than 25,000 ESTs and described 13,814 unique transcripts from eight different species representing the biodiversity of this genus: T. harzianum, T. virens, T. atroviride, T. asperellum, T. viride, T. longibrachiatum, T. stromaticum, and T. aggressivum (99). The ESTs were from 28 cDNA libraries obtained under a wide range of growth conditions, including biocontrol interactions and nutrient stress (140). A subset of 8710 ESTs, from eight T. harzianum CECT2413 (T. harzianum T34) cDNA libraries, revealed 3478 unique sequences.…”

Section: Expressed Sequence Tags (Ests)mentioning

confidence: 99%

Translational Research on Trichoderma: From 'Omics to the Field

Lorito

Woo

Harman

et al. 2010

Annu. Rev. Phytopathol.

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536

372

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Structural and functional genomics investigations are making an important impact on the current understanding and application of microbial agents used for plant disease control. Here, we review the case of Trichoderma spp., the most widely applied biocontrol fungi, which have been extensively studied using a variety of research approaches, including genomics, transcriptomics, proteomics, metabolomics, etc. Known for almost a century for their beneficial effects on plants and the soil, these fungi are the subject of investigations that represent a successful case of translational research, in which 'omics-generated novel understanding is directly translated in to new or improved crop treatments and management methods. We present an overview of the latest discoveries on the Trichoderma expressome and metabolome, of the complex and diverse biotic interactions established in nature by these microbes, and of their proven or potential importance to agriculture and industry.

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Section: Expressed Sequence Tags (Ests)mentioning

confidence: 99%

Translational Research on Trichoderma: From 'Omics to the Field

Lorito

Woo

Harman

et al. 2010

Annu. Rev. Phytopathol.

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536

372

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“…Trichoderma spp. can also exert marked antimicrobial activity (Vizcaíno et al ., ) as a result of the production of a huge diversity of secondary metabolites (Cardoza et al ., ; Reino et al ., ), including, for example, pyrones [6‐pentyl‐2H‐pyran‐2‐one (6‐PP) derivatives] (Keszler et al ., ; Rubio et al ., ), butenolides (Cai et al ., ), peptaibols (Vizcaíno et al ., 2005b; 2006), and trichothecenes (terpenes) (Cardoza et al ., ; Malmierca et al ., 2012; 2013).…”

Section: Introductionmentioning

confidence: 99%

Production of trichodiene by Trichoderma harzianum alters the perception of this biocontrol strain by plants and antagonized fungi

Malmierca

McCormick

Cardoza

et al. 2014

Environmental Microbiology

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Trichothecenes are phytotoxic sesquiterpenic mycotoxins that can act as virulence factors in plant diseases. Harzianum A (HA) is a non-phytotoxic trichothecene produced by Trichoderma arundinaceum. The first step in HA biosynthesis is the conversion of farnesyl diphosphate to trichodiene (TD), a volatile organic compound (VOC), catalysed by a sesquiterpene synthase encoded by the tri5 gene. Expression of tri5 in the biocontrol strain Trichoderma harzianum CECT 2413 resulted in production of TD in parallel with a reduction of ergosterol biosynthesis and an unexpected increase in the level of squalene. Transformants expressing tri5 displayed low chitinase activity and induced expression of Botrytis cinerea BOT genes, although their total antagonistic potential against phytopathogenic fungi was not reduced. VOCs released by the tri5-transformant induced expression of tomato defence genes related to salicylic acid (SA), and TD itself strongly induced the expression of SA-responsive genes and reduced the development of lateral roots. Together, these results suggest that TD acts as a signalling VOC in the interactions of Trichoderma with plants and other microorganisms by modulating the perception of this fungus to a given environment. Moreover, the TD ability to induce systemic defences indicates that complex trichothecene structures may not be necessary for inducing such responses.

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“…Two bands of 21 and 32 kDa (indicated by arrowheads) were observed consistently in all the strips. M molecular weight markers, C crude extract of T. harzianum on 12% gel consistent with the outcomes of a genome analysis study of T. harzianum strain CECT 2413 (Vizcaíno et al 2006) where a total of 3,478 unique sequences were identified with one having sequence similarity with Alt a 5, an allergen reported from Alternaria alternata (I.U.I.S. 2007).…”

Section: Discussionmentioning

confidence: 92%

Trichoderma harzianum: occurrence in the air and clinical significance

Das

Gupta‐Bhattacharya

2009

Aerobiologia

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Trichoderma harzianum, a filamentous fungus, is being widely used as a potential biopesticide. The potential of this fungus in causing skin sensitization, however, was poorly investigated as yet. The objective of this study was to monitor the occurrence of T. harzianum in the air and to explore its skin sensitizing potential. Seasonal periodicity of T. harzianum was studied for the years 2002-2004 by an Andersen air sampler. The skin sensitizing potential of T. harzianum extract was studied in 389 patients with suspected respiratory allergy by skin prick test (SPT) and specific IgE level was determined by ELISA. SDS-PAGE and immunoblotting were also performed. T. harzianum colony count varied from 3.69 to 134.88 CFU m -3 with the peak achieved in February. Relative humidity was found to be a significant (P \ 0.05) factor predicting the occurrence of T. harzianum in the air. Positive skin reaction (wheal diameter C 3 mm) was observed in 105 patients (26.99%). T. harzianum crude extract was resolved in 18 protein bands (12-72 kDa) on SDS-PAGE (12% gel) including two IgE-binding protein bands (21 and 32 kDa). T. harzianum can be considered an important inhalant allergen.

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Generation, annotation and analysis of ESTs from Trichoderma harzianum CECT 2413

Cited by 62 publications

References 59 publications

Translational Research on Trichoderma: From 'Omics to the Field

Translational Research on Trichoderma: From 'Omics to the Field

Production of trichodiene by Trichoderma harzianum alters the perception of this biocontrol strain by plants and antagonized fungi

Trichoderma harzianum: occurrence in the air and clinical significance

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