Generation of an induced pluripotent stem cell (iPSC) line from a 42-year-old adult cerebral type X-linked adrenoleukodystrophy (X-ALD) patient

Yeon, Gyu Bum; Son, Daryeon; Kang, Phil Jun; Oh, Seung Min; Lim, Hun; Kang, Hoon Chul; You, Seungkwon; Kim, Dae Sung

doi:10.1016/j.scr.2019.101425

Cited by 2 publications

(1 citation statement)

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“…After 10 passages, the cells were characterized by immunocytochemistry (OCT4, NANOG, and SSEA4) and gene expression analysis for pluripotency markers (OCT4, c-MYC, NANOG, and SRY-box transcription factor 2 [SOX2]) (Figure S1a,b). One hiPSC line that showed the most promising results for the expression of the pluripotency marker, named NL1, was subjected to in vitro differentiation via embryoid body formation following a protocol described previously (Yeon et al, 2019). Spontaneous differentiation of the newly established hiPSCs for 2 weeks generated the cells from three germ layers, positive for SOX1 (ectoderm), Brachyury (mesoderm), and α-fetoprotein (endoderm) (Figure S1c).…”

Section: Generation and Characterization Of Hipscsmentioning

confidence: 99%

NFIB induces functional astrocytes from human pluripotent stem cell‐derived neural precursor cells mimicking in vivo astrogliogenesis

Yeon

Shin

Yoo

et al. 2021

Journal Cellular Physiology

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The ability to generate astrocytes from human pluripotent stem cells (hPSCs) offers a promising cellular model to study the development and physiology of human astrocytes. The extant methods for generating functional astrocytes required long culture periods and there remained much ambiguity on whether such paradigms follow the innate developmental program. In this report, we provided an efficient and rapid method for generating physiologically functional astrocytes from hPSCs.Overexpressing the nuclear factor IB in hPSC-derived neural precursor cells induced a highly enriched astrocyte population in 2 weeks. RNA sequencing and functional analyses demonstrated progressive transcriptomic and physiological changes in the cells, resembling in vivo astrocyte development. Further analyses substantiated previous results and established the MAPK pathway necessary for astrocyte differentiation. Hence, this differentiation paradigm provides a prospective in vitro model for human astrogliogenesis studies and the pathophysiology of neurological diseases concerning astrocytes.

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Section: Generation and Characterization Of Hipscsmentioning

confidence: 99%