Generation of human tonsil epithelial organoids as an ex vivo model for SARS-CoV-2 infection

Kim, Han Kyung; Kim, Hyeryeon; Lee, Myoung Kyu; Choi, Woo Hee; Jang, Yejin; Shin, Jin Soo; Park, Jun-Yeol; Bae, Dong Hyuck; Hyun, Seong-In; Kim, Kang Hyun; Han, Hyun Wook; Lim, Byungho; Choi, Gildon; Kim, Meehyein; Lim, Young Chang; Yoo, Jongman

doi:10.1016/j.biomaterials.2022.121460

Cited by 24 publications

(16 citation statements)

References 72 publications

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“…64 Interestingly, this drug was also utilized as confirmation of the development of human tonsillar epithelial organoids as an ex vivo model to study viral infections, such as SARS-CoV-2. 65 It was found that remdesivir could successfully decrease viral RNA in these organoid cultures in a dose-dependent manner, indicating that these novel type organoids could be used as a preclinical and translational research platform for testing of future clinical interventions.…”

Section: Drug Testing and Developmentmentioning

confidence: 97%

Human lung organoids as a model for respiratory virus replication and countermeasure performance in human hosts

Edwards

Tata

Baric

2022

Translational Research

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Section: Drug Testing and Developmentmentioning

confidence: 97%

Human lung organoids as a model for respiratory virus replication and countermeasure performance in human hosts

Edwards

Tata

Baric

2022

Translational Research

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“…Kim et al demonstrated their expression of key SARS-CoV-2 entry molecules—ACE2, TMPRSS2, and furin, enabling organoid infection and robust replication efficiency. These results prove that tonsil organoids can also serve as suitable ex vivo models of SARS-CoV-2 infection [ 142 ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 84%

Induced Pluripotent Stem Cell-Derived Organoids: Their Implication in COVID-19 Modeling

Csöbönyeiová

Klein

Kuniaková

et al. 2023

IJMS

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The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a significant global health issue. This novel virus’s high morbidity and mortality rates have prompted the scientific community to quickly find the best COVID-19 model to investigate all pathological processes underlining its activity and, more importantly, search for optimal drug therapy with minimal toxicity risk. The gold standard in disease modeling involves animal and monolayer culture models; however, these models do not fully reflect the response to human tissues affected by the virus. However, more physiological 3D in vitro culture models, such as spheroids and organoids derived from induced pluripotent stem cells (iPSCs), could serve as promising alternatives. Different iPSC-derived organoids, such as lung, cardiac, brain, intestinal, kidney, liver, nasal, retinal, skin, and pancreatic organoids, have already shown immense potential in COVID-19 modeling. In the present comprehensive review article, we summarize the current knowledge on COVID-19 modeling and drug screening using selected iPSC-derived 3D culture models, including lung, brain, intestinal, cardiac, blood vessels, liver, kidney, and inner ear organoids. Undoubtedly, according to reviewed studies, organoids are the state-of-the-art approach to COVID-19 modeling.

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“…Rotational motion of organoids could be evaluated, demonstrating a functional readout that can be used for assessing patient-specific cilia defects ( Wijesekara et al, 2022 ). The development of organoids from human tonsil tissue has been reported very recently ( Kim et al, 2022 ). In this study, purified tonsil epithelial cells were efficiently cultured in 3D and expanded as organoids in an optimised tonsil organoid medium.…”

Section: Lung Upper Airways and Tonsilsmentioning

confidence: 99%

“…Furthermore, to examine the applicability as a functional ex-vivo disease model, tonsil organoids were infected with SARS-CoV-2. Tonsil organoids were found to be responsive to the viral infection and could be used to evaluate the therapeutic efficacy of antiviral treatment, thus demonstrating their relevance as a disease modelling and drug testing platform ( Kim et al, 2022 ).…”

Section: Lung Upper Airways and Tonsilsmentioning

confidence: 99%

Primary human organoids models: Current progress and key milestones

Calà

Sina²,

Coppi³

et al. 2023

Front. Bioeng. Biotechnol.

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During the past 10 years the world has experienced enormous progress in the organoids field. Human organoids have shown huge potential to study organ development, homeostasis and to model diseases in vitro. The organoid technology has been widely and increasingly applied to generate patient-specific in vitro 3D cultures, starting from both primary and reprogrammed stem/progenitor cells. This has consequently fostered the development of innovative disease models and new regenerative therapies. Human primary, or adult stem/progenitor cell-derived, organoids can be derived from both healthy and pathological primary tissue samples spanning from fetal to adult age. The resulting 3D culture can be maintained for several months and even years, while retaining and resembling its original tissue’s properties. As the potential of this technology expands, new approaches are emerging to further improve organoid applications in biology and medicine. This review discusses the main organs and tissues which, as of today, have been modelled in vitro using primary organoid culture systems. Moreover, we also discuss the advantages, limitations, and future perspectives of primary human organoids in the fields of developmental biology, disease modelling, drug testing and regenerative medicine.

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Generation of human tonsil epithelial organoids as an ex vivo model for SARS-CoV-2 infection

Cited by 24 publications

References 72 publications

Human lung organoids as a model for respiratory virus replication and countermeasure performance in human hosts

Human lung organoids as a model for respiratory virus replication and countermeasure performance in human hosts

Induced Pluripotent Stem Cell-Derived Organoids: Their Implication in COVID-19 Modeling

Primary human organoids models: Current progress and key milestones

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