Generation of immortalized human naïve B cell libraries by optimized EBV transformation

Lu, Shiqiang; Sun, Zehua; Zhang, Mei-yun

doi:10.24262/jmd.2.1.17007

Cited by 2 publications

(1 citation statement)

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“…However, due to the low efficiency of EBV-induced B cell transformation, this method has limitation in application. Though optimization methods were studied based on EBV-induced B cell transformation, the efficiency still needs a dramatic improve (Sun et al, 2017;Lu et al, 2016;Kwakkenbos et al, 2016). Some reports show that memory B cells can be cultured short-term, and the supernatant can be used directly for screening of neutralization activity in a high throughput neutralization assay (micro-neutralization).…”

Section: Hybridoma Technique B Cell Immortalization and Microneutralmentioning

confidence: 99%

Brief introduction of current technologies in isolation of broadly neutralizing HIV-1 antibodies

Sun

Yan

Tang³

et al. 2018

Virus Research

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HIV/AIDS has become a worldwide pandemic. Before an effective HIV-1 vaccine eliciting broadly neutralizing monoclonal antibodies (bnmAbs) is fully developed, passive immunization for prevention and treatment of HIV-1 infection may alleviate the burden caused by the pandemic. Among HIV-1 infected individuals, about 20% of them generated cross-reactive neutralizing antibodies two to four years after infection, the details of which could provide knowledge for effective vaccine design. Recent progress in techniques for isolation of human broadly neutralizing antibodies has facilitated the study of passive immunization. The isolation and characterization of large panels of potent human broadly neutralizing antibodies has revealed new insights into the principles of antibody-mediated neutralization of HIV. In this paper, we review the current effective techniques in broadly neutralizing antibody isolation.

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Section: Hybridoma Technique B Cell Immortalization and Microneutralmentioning

confidence: 99%

Brief introduction of current technologies in isolation of broadly neutralizing HIV-1 antibodies

Sun

Yan

Tang³

et al. 2018

Virus Research

Self Cite

View full text Add to dashboard Cite

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Characterization of a Monoclonal Antibody That Binds to Both gp120 and gp41

Zheng

Tang²,

et al. 2018

Future Virol.

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Aim: AIDS has become a global pandemic. Characterization of broadly HIV-1-neutralizing antibodies (bnAbs) may facilitate the vaccine design. Methods & materials: Recombinant antibody library construction provides a resourceful way of monoclonal antibody screening and isolation against HIV-1. Results: In this study, we screened a novel human monoclonal antibody, named 2B8, which can bind both the gp120 and gp41 subunits of the HIV-1 envelope glycoprotein (Env). 2B8 did not bind to the CD4 binding site mutant, gp120 D368R, suggesting that the 2B8 epitope is conformational and overlaps the CD4 binding site on gp120. 2B8 neutralized 50% of the HIV-1 cell line-based pseudo virus isolates tested. Conclusion: The further study of its novel epitope may reveal the new mechanism of neutralization and assist the design of vaccine immunogens against HIV-1.

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Generation of immortalized human naïve B cell libraries by optimized EBV transformation

Cited by 2 publications

References 0 publications

Brief introduction of current technologies in isolation of broadly neutralizing HIV-1 antibodies

Brief introduction of current technologies in isolation of broadly neutralizing HIV-1 antibodies

Characterization of a Monoclonal Antibody That Binds to Both gp120 and gp41

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