Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction

Thirukkumarana, Gunaratnam; Ntui, Valentine Otang; Khan, Raham Sher; Nakamura, Ikuo; Mii, Masahiro

doi:10.5511/plantbiotechnology.27.147

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…MAT vectors have been refined over time, for instance by optimizing the expression of the ipt or rol genes, or by placing recombinase gene expression under the control of a glutathione-S-transferase promoter that is inducible by an herbicide (Sugita et al, 2000a;. The MAT vector system has been successfully tested in many plant species including tobacco ), hybrid aspen (Matsunaga et al, 2002), rice (Sugita et al, 2005), apricot (Lopez-Noguera et al, 2006;Lopez-Noguera et al, 2009), citrus (Ballester et al, 2008), cassava (Saelim et al, 2009), white poplar (Balestrazzi et al, 2009), Kalanchoe blossfeldiana (Thirukkumaran et al, 2010), tomato (Khan et al, 2011b), potato (Khan et al, 2011b;Khan et al, 2011c) and Petunia hybrida (Khan et al, 2011a). It shows very different levels of efficiency, and the use of the ipt SMG may require the optimization of transformation protocols due to changes in tissue culture conditions.…”

Section: The Mat Vector Systemmentioning

confidence: 99%

Alternatives to Antibiotic Resistance Marker Genes forIn VitroSelection of Genetically Modified Plants – Scientific Developments, Current Use, Operational Access and Biosafety Considerations

Breyer

Kopertekh

Reheul

2014

Critical Reviews in Plant Sciences

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Section: The Mat Vector Systemmentioning

confidence: 99%

Alternatives to Antibiotic Resistance Marker Genes forIn VitroSelection of Genetically Modified Plants – Scientific Developments, Current Use, Operational Access and Biosafety Considerations

Breyer

Kopertekh

Reheul

2014

Critical Reviews in Plant Sciences

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Horizontal Gene Transfer Through Genetic Transformation

Bhatnagar‐Mathur

Palit

Sharma

2013

Alien Gene Transfer in Crop Plants, Volume 1

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The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

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Recent advances in development of marker-free transgenic plants: Regulation and biosafety concern

et al. 2012

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During the efficient genetic transformation of plants with the gene of interest, some selectable marker genes are also used in order to identify the transgenic plant cells or tissues. Usually, antibiotic- or herbicide-selective agents and their corresponding resistance genes are used to introduce economically valuable genes into crop plants. From the biosafety authority and consumer viewpoints, the presence of selectable marker genes in released transgenic crops may be transferred to weeds or pathogenic microorganisms in the gastrointestinal tract or soil, making them resistant to treatment with herbicides or antibiotics, respectively. Sexual crossing also raises the problem of transgene expression because redundancy of transgenes in the genome may trigger homology-dependent gene silencing. The future potential of transgenic technologies for crop improvement depends greatly on our abilities to engineer stable expression of multiple transgenic traits in a predictable fashion and to prevent the transfer of undesirable transgenic material to non-transgenic crops and related species. Therefore, it is now essential to develop an efficient marker-free transgenic system. These considerations underline the development of various approaches designed to facilitate timely elimination of transgenes when their function is no longer needed. Due to the limiting number of available selectable marker genes, in future the stacking of transgenes will be increasingly desirable. The production of marker-free transgenic plants is now a critical requisite for their commercial deployment and also for engineering multiple and complex trait. Here we describe the current technologies to eliminate the selectable marker genes (SMG) in order to develop marker-free transgenic plants and also discuss the regulation and biosafety concern of genetically modified (GM) crops.

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Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction

Cited by 4 publications

References 39 publications

Alternatives to Antibiotic Resistance Marker Genes forIn VitroSelection of Genetically Modified Plants – Scientific Developments, Current Use, Operational Access and Biosafety Considerations

Alternatives to Antibiotic Resistance Marker Genes forIn VitroSelection of Genetically Modified Plants – Scientific Developments, Current Use, Operational Access and Biosafety Considerations

Horizontal Gene Transfer Through Genetic Transformation

Recent advances in development of marker-free transgenic plants: Regulation and biosafety concern

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