Generation of precursor cell lines from preneoplastic fields surrounding head and neck cancers

Abstract

Our data show that noninvasive fields surrounding HNSCC may consist of immortalized preneoplastic cell clones. Our preneoplastic cell line is a valuable tool to develop and test treatment strategies for precursor fields in patients with HNSCC.

Deregulation of SYCP2 predicts early stage human papillomavirus‐positive oropharyngeal carcinoma: A prospective whole transcriptome analysis

“…(34) In particular, further characterization of a pre-malignant state in the development of HPV-associated OPSCC would be of clinical value as it infers the potential for a screening test (similar to the cervical carcinoma model). In HNSCC surgical excisions, dysplastic epithelium is often found adjacent to the cancer, (10) but the exact nature of the disease in these regions is poorly defined. (12,13) Within our retrospective LCM cohort, 70% of patients showed significantly increased expression of CDKN2A (a proxy marker for HPV infection) in regions of carcinoma in situ relative to normal epithelium.…”

“…In particular, further characterization of a pre‐malignant state in the development of HPV‐associated OPSCC would be of clinical value as it infers the potential for a screening test (similar to the cervical carcinoma model). In HNSCC surgical excisions, dysplastic epithelium is often found adjacent to the cancer, but the exact nature of the disease in these regions is poorly defined …”

“…(8,9) Oropharyngeal squamous cell carcinogenesis involves progressive transformation of normal epithelium into premalignant tissue (dysplasia ⁄ carcinoma in situ) and, ultimately, invasive cancer. (10)(11)(12)(13) Although the presence of HPV subtypes within invasive oropharyngeal SCC has been evaluated in large epidemiological studies, (1,14) there is limited data on this subject in regions of confirmed dysplasia ⁄ carcinoma in situ. Prior studies have reported markedly variable HPV prevalence rates due to limitations of size and variable assay techniques.…”

Characterization of preneoplastic and neoplastic rat mesothelial cell lines: the involvement of TETs, DNMTs, and 5-hydroxymethylcytosine

“…To date, most studies on preneoplastic cell lines have been conducted either on spontaneously transformed normal cells in culture [27] or on immortalized cell clones isolated from biopsies [28]. Additional insights have been obtained from histopathological observations made on both preneoplastic and neoplastic cells [29] and by the use of transgenic animals [30].…”

Surgical margins in head and neck squamous cell carcinoma: Effect of heat artifact on immunohistochemistry as a future tool for assessment

“…Increasingly, evidence in the literature demonstrates that this method of assessment does not reveal the full picture. 3,4,6,9,[11][12][13][14][15] Immunohistochemistry and various DNA techniques have both been shown by multiple authors to have potential. [8][9][10] This undetected change in biological nature may explain why positive margins are not always associated with local recurrence and a clear margin does not guarantee cure.…”