Generic approach to chiral separations: Chiral capillary electrophoresis with ternary cyclodextrin mixtures

Abstract

A generic approach to chiral separations using capillary electrophoresis is outlined. The generic method was developed by investigating the interactions of Ž . charged sulfobutyl ether-␤-cyclodextrin, SBE-␤-CD and neutral cyclodextrins Ž . Ž primarily ␥and dimethyl-␤-cyclodextrin in a low pH buffer 50 mM sodium . Ž . dihydrogen phosphate, pH 2.5 . Using a background electrolyte BGE containing Ž . 5% wrw SBE-␤-CD, 25 mM ␥-cyclodextrin ␥-CD and 25 mM dimethyl-␤-CD Ž . DM-␤-CD dissolved in the phosphate buffer, resolution of more than 90% of the 26 randomly selected racemic pharmaceutical drugs or their synthetic intermediates was achieved.

Chiral Separations

“…Differing CD selector concentrations was again the focus of a study by Amini et al (38), in which the partial filling technique was employed and evaluated. A generic approach to chiral CE separations using ternary CD mixtures to enantioseparate 26 randomly selected racemic pharmaceutical drugs or their synthetic intermediates was presented by Szucs et al (39) A dual-CD system in which mixtures of neutral and negatively charged CDs were used to separate cationic drugs by chiral CE was also found to enhance the peak symmetry of the second-migrating enantiomer in a study by Grard et al (40). The simultaneous chiral separation of two triazole fungicides was investigated in a sulfated-β-CD CE system (41).…”

Comparison of Chiral Chromatography Columns for Pharmaceutical Method Development

“…Elaborate screening procedures for assisted method development for liquid [2][3][4][5][6][7] and supercritical fluid [8][9][10][11][12][13][14] chromatography can be found in the literature. Capillary electrophoresis has been studied for chiral applications but is used to a lesser extent in the pharmaceutical industry, due primarily to cost and sensitivity concerns [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. Chiral gas chromatography is used principally for raw materials and early intermediates, as most drug entities are not volatile [33,34].…”