Genetic analysis of barrage line formation during mycelial incompatibility in Rosellinia necatrix

Cytological analysis of mycelial incompatibility in Rosellinia necatrix

“…In R. necatrix, we found distinct barrage lines between incompatible pairs (Ikeda et al 2011). The barrage lines consisted of degraded mycelia, pigmented pseudosclerotia, fuzzy mycelia, and tuft-like mycelia.…”

“…328106) and w779 (MCG351, collected from Ibaraki prefecture, MAFF No. 328195), which possesses a virocontrol agent (Chiba et al 2009), and single-ascospore isolates from the same perithecium (isolates 17, 45, 112, and 115;Ikeda et al 2011). All isolates were self-compatible, but were incompatible with the hyphae of other isolates.…”

Potentiation of Mycovirus Transmission by Zinc Compounds via Attenuation of Heterogenic Incompatibility in Rosellinia necatrix

“…Successful transmission of mycovirus was confirmed by detection of dsRNA. The mycelium on the cellulose membrane was placed into an extraction tube set (lysing matrix set A; MP Biomedicals, Santa Ana, CA) with 1 ml of nucleic acid extraction buffer (17), and dsRNA was extracted by using a FastPrep 24 instrument (MP Biomedicals). The extract was centrifuged (10,000 ϫ g), and the supernatant was transferred.…”

“…Genomic DNA was extracted according to the method of Ikeda et al (17). To exclude contamination with donor mycelia during dsRNA extraction, universally primed PCR (UP-PCR) was performed to detect DNA polymorphisms (17,(27)(28)(29) by using primer AS4 (5=-TGTGGGCGCTCGACAG). PCR products were separated by electrophoresis on a 1.0% agarose gel in TAE buffer.…”

“…Numerous R. necatrix isolates belonging to different mycelial compatibility groups (MCGs) have been isolated previously (16). Moreover, ascospore isolates were obtained from a single ascus, and most ascospore isolates were shown to produce a barrage line paired with sibling ascospore isolates (17). Pyramiding with heterogenic incompatibility genes may produce a strong incompatibility reaction.…”