Genetic and antigenic studies and partial purification of a human serum lipoprotein carrying the Lp antigenic determinant.

Schultz, Jerome S.; Shreffler, Donald C.; Harvie, Nancy Reid

doi:10.1073/pnas.61.3.963

Cited by 29 publications

(5 citation statements)

References 8 publications

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“…In normal subjects, -5% of apo B-100 is found in VLDL, with nearly all of the remainder in the IDL and LDL density range (26,33). A small amount of apo B-100 can also be found in Lp(a) particles in the 1.070-1.090 g/ml density range (39,40).…”

Section: Discussionmentioning

confidence: 99%

Characterization of an abnormal species of apolipoprotein B, apolipoprotein B-37, associated with familial hypobetalipoproteinemia.

Young¹,

Bertics²,

Curtiss³

et al. 1987

J. Clin. Invest.

114

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Steinberg and colleagues have previously described a unique kindred with normotriglyceridemic hypobetalipoproteinemia (1979. J. Clin. Invest. 64:292-301). In a reexamination of this kindred, we found an abnormal apolipoprotein (apo) B species, apo B-37 (203,000 mol wt), in the plasma lipoproteins of multiple members of the kindred. In affected individuals apo B-37 was found in very low density lipoproteins, along with the normal apo B species, apo B-100 and apo B-48. High density lipoproteins (HDL) also contained apo B-37, but no other apo B species. The first 13 amino-terminal amino acids of apo B-37 were identical to those of normal apo B-100. We utilized a panel of 18 different apo B-specific monoclonal antibodies and polyclonal antisera specific for apo B-37 and the thrombin cleavage products of apo B-100 to map apo B-37 in relation to apo B-100, apo B-48, and the thrombin cleavage products of apo B-100. The results of those immunochemical studies indicated that apo B-37 contains only amino-terminal domains of apo B-100.In affected individuals, the majority of apo B-37 in plasma was contained in the HDL density fraction. Within that fraction apo B-37 was found on discrete lipoprotein particles, termed Lp-B37, that had properties distinct from normal HDL particles containing apo A-I.This report documents for the first time the existence of an abnormal apo B species in humans. Further study of apo B-37 and lipoprotein particles containing apo B-37 should lead to an improved understanding of apo B structure and function.

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Section: Discussionmentioning

confidence: 99%

Characterization of an abnormal species of apolipoprotein B, apolipoprotein B-37, associated with familial hypobetalipoproteinemia.

Young¹,

Bertics²,

Curtiss³

et al. 1987

J. Clin. Invest.

114

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“…Other investigators have confirmed this finding (Utermann and Wiegandt, 1970). The Lp lipoprotein has been isolated from human serum by various methods involving precipitation with dextran sulfate, preparative and density gradient ultracentrifugation, and column chromatography (Wiegandt et al, 1968;Schultz et al, 1968;Ehnholm et al, 1971;Simons et al, 1970;Utermann and Wiegandt, 1969;Utermann et al, 1972).…”

Section: Introductionmentioning

confidence: 86%

“…Specific Lp(a) antisera were prepared~ absorbed, and checked by gel diffusion analysis as described previously (Schultz et al, 1968). Two antisera were used, one prepared against a double Lp variant, called anti-double Lp, and another prepared against a single Lp variant, called anti-single Lp.…”

Section: Antiseramentioning

confidence: 99%

“…The Lp lipoprotein has a density between 1.064 and 1.12 g/ml, an Sf 1.21, 20 c of approximately 24, and an estimated molecular weight of 5 × 105 (Schultz et al, 1968;Simons et al, 1970;Ehnholm et al, 1971). Preliminary biochemical data indicate that Lp lipoprotein has approximately 40% protein and a higher carbohydrate content than LDL 4 (Ehnholm et al, 1972).…”

Section: Introductionmentioning

confidence: 97%

“…Preliminary biochemical data indicate that Lp lipoprotein has approximately 40% protein and a higher carbohydrate content than LDL 4 (Ehnholm et al, 1972). The Lp lipoprotein shares some antigenic determinants with LDL, contains other(s) specific for Lp(a), and shows no cross-reactivity with HDL 4 (Schultz et al, 1968;Wiegandt et al, 1968). Recently it has been reported by two laboratories (Utermann et al~ 1972;Ehnholm et al, 1972) that Lp lipoprotein disaggregated after standing, or after treatment with detergent, into three components, i.e., LDL lipoprotein, Lp(a) apoprotein, and albumin.…”

Section: Introductionmentioning

confidence: 99%

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Studies on the heterogeneity of human serum Lp lipoproteins and on the occurrence of double Lp lipoprotein variants

Harvie

Schultz

1973

Biochem Genet

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Lp lipoproteins have been prepared by a mild method from the serum of a large number of individuals. Approximately 25%o of the individuals tested showed the presence of a double Lp peak in analytical ultracentrifuge diagrams. These double peaks were designated Lp(a)-I and Lp(a)-2 to distinguish them from the single Lp(a) peak. The mean viscosity-corrected sedimentation coefficient, sl.oo4, 20 c, and density of the single Lp(a) peak were 15.8 +_ 1.8s (n = 32) and 1.076++_0.01 g/ml, of the Lp(a)-I peak were 13.5+_1.1s (n = 14) and 1.064+ 0.007 g/ml, and of the Lp(a)-2 peak were 16.8 +-1.7s (n = 14) and 1.074 +-0.009 g/ml. Absorption tests using a double and single Lp preparation showed that both Lp peaks in the double variants possess Lp(a) specificity. Evidence is lacking as yet for individual specificities for either Lp(a)-I or Lp(a)-2. Interand intra-individual heterogeneity among Lp lipoproteins is discussed.

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Elevated Plasma Lipoprotein(a) and Coronary Heart Disease in Men Aged 55 Years and Younger

Bostom

Cupples²,

Jenner³

et al. 1996

JAMA

299

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Genetic and antigenic studies and partial purification of a human serum lipoprotein carrying the Lp antigenic determinant.

Cited by 29 publications

References 8 publications

Characterization of an abnormal species of apolipoprotein B, apolipoprotein B-37, associated with familial hypobetalipoproteinemia.

Characterization of an abnormal species of apolipoprotein B, apolipoprotein B-37, associated with familial hypobetalipoproteinemia.

Studies on the heterogeneity of human serum Lp lipoproteins and on the occurrence of double Lp lipoprotein variants

Elevated Plasma Lipoprotein(a) and Coronary Heart Disease in Men Aged 55 Years and Younger

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