Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers

Al-Khayri, Jameel M.; Mahdy, Ehab M. B.; Taha, Heba; Eldomiaty, Ahmed S.; Abd-Elfattah, Mohamed A.; Latef, Arafat Abdel Hamed Abdel; Rezk, Adel A.; Shehata, Wael F.; Almaghasla, Mustafa I.; Shalaby, Tarek; Sattar, Muhammad Naeem; Ghazzawy, Hesham S.; Awad, Mohamed F.; Alali, Khalid M.; Jain, S. Mohan; Hassanin, Abdallah A.

doi:10.3390/plants11131722

Cited by 33 publications

(17 citation statements)

References 41 publications

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“…A set of 22 ISSRs have been used to expedite the molecular breeding of oats. The high cross-transferability and polymorphism of these ISSR markers further reveal their novelty [ 31 ]. Utilization of these novel ISSRs markers in diversity analysis and population structure characterization of 38 oat germplasms suggest their wider efficacy on a superior scale for molecular breeding studies in oats.…”

Section: Resultsmentioning

confidence: 99%

Agro-morphological and genetic variability analysis in oat germplasms with special emphasis on food and feed

Kumar

Varghese

Jayaswal³

et al. 2023

PLoS ONE

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The gaining attention of underutilized oat crops for both food and feed, mining of quality and yield related genes/QTLs from available germplasms of oat is need of the hour. The large family of grasses has a vast number of germplasms that could be harnessed for bio-prospecting. The selection of cross-compatible oat germplasms by molecular markers could be used for the introgression of the novel traits into the elite background of oats. The process needs a thorough study of genetic diversity to see the evolutionary relatedness among germplasms. Considering this, in the present study, the genetic diversity of 38 oat germplasms with 12 agro-morphological traits was carried out using 22 Inter Simple Sequence Repeat (ISSR) markers. We found a high level of polymorphism and 158 distinctive alleles; on average 7.18 alleles per primer, further, high-yielding genotypes were identified with the help of phenotypic data and genetic diversity was analyzed by using DNA fingerprint-based principal component analysis, UPGMA dendrogram. Among these 38 germplasms; eight were identified as superior under high grain yield (OS-424, OS-403, NDO-1101, OL-10, UPO-212, OS-405, OS-6, and OS-346) and another eight germplasms were identified as superior for the high fresh weight (for fodder purpose, NDO-711, RO-19, OL-14, OL-1760/OL-11, NDO-10, UPO-212, UPO-06-1, and RO-11-1). These results suggest that germplasms that are closely related (Cross-compatible) and have good potential for desirable traits could be used for varietal development by using marker-assisted selection.

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Section: Resultsmentioning

confidence: 99%

Agro-morphological and genetic variability analysis in oat germplasms with special emphasis on food and feed

Kumar

Varghese

Jayaswal³

et al. 2023

PLoS ONE

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“…Understanding the nature and degree of genetic variety in crops requires non-overlapping and complementary morphological and molecular data that are highlighted by the absence of relationship between the diversity matrices (grey lines). Several researches investigated the discrepancies between phenotypic and genotypic characteristics, such as maize ( Hartings et al., 2008 ), wheat ( Soriano et al., 2016 ), sweet sorghum ( Da Silva et al., 2017 ), kalanchoe ( Al-Khayri et al., 2022 ), sesame ( Stavridou et al., 2021 ). An entanglement rate of 0.65 was obtained among 77 passion fruit genotypes showing dissimilarities between the molecular and agronomic data ( Preisigke et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Cyclamen genotypes using morphological descriptors and DNA molecular markers in a multivariate analysis

Cornea-Cipcigan

Pamfil²,

Sisea³

et al. 2023

Front. Plant Sci.

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Morphological and molecular characterization of germplasm is essential for the improvement of cultivated plants efforts. This study investigated the genetic diversity of 32 Cyclamen genotypes comprising 16 C. persicum varieties and 16 Cyclamen species using multivariate analysis for 36 morphological traits (19 quantitative and 17 qualitative) and molecular characterization (SRAP and SCoT markers). The color CIELab parameters were collected via PCE-CSM7 that separately measured the leaves dark and silvery patterns and the flower’s slip (region of the petal top), eye (the region of the petal base) and sepal. Genetic diversity was also evaluated using Shannon Wiener (H′) and Simpson’s (λ) Indices, and Pilou evenness (J) using the library vegan from R software. According to the principal component analysis, the variables that contributed the most were leaf pattern color, leaf abaxial surface color, pedicel coiling, leaf and stem number. The color indicators of Cyclamen leaves showed decreased L* values in darker colored genotypes, whereas increased a* values were noticed in flower eye and lower in slip. Molecular characterization was based on 26 SRAP and 12 SCoT markers that produced clearly repeatable DNA bands and exhibited significant levels of polymorphism. Based on the morphological traits and molecular markers data, the UPGMA method for hierarchical clustering technique was used to generate the dendrograms, and their entanglement was obtained using the Tanglegram algorithm from the dendextend package with the R software. Entanglement analysis (0.30) between dendrograms obtained from the morphological and genetic analysis using SRAP markers showed a high association. Comparison between color measurements of flowers (entanglement=0.45) and leaves (entanglement=0.47) with SCoT analysis revealed differences at species level, discriminating between similar genotypes. Combined phenotypic and molecular analysis improved the comprehensive estimation of real diversity in the investigated Cyclamen genotypes. The findings of the present study are useful for quantifying diversity and genetic variability in Cyclamen breeding and genetic investigations.

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“…The improvement of date palm production not only depends on the in vitro propagation techniques but also the use of chemical and physical mutagens [ 33 ], the recent genetic approaches [ 34 , 35 ], phylogeny analysis [ 36 ], and molecular markers [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation of Three Date Palm (Phoenix dactylifera L.) Varieties Using Immature Female Inflorescences

Abdelghaffar

Soliman

Ismail

et al. 2023

Plants

Self Cite

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Immature female inflorescences are promising materials for use as explants for the tissue culture of date palm. Four types of MS media were used in this study during the four micropropagation stages—starting media (SM), maturation media (MM), multiplication media (PM) and rooting media (RM)—to micropropagate three elite date palm varieties, Amri, Magdoul and Barhy using the immature female inflorescences as explant. The highest percentage of callus induction in all the varieties studied was obtained on the SM1 (9 µM 2,4-D + 5.7 µM IAA + 10 µM NAA). Culturing on the MM1 (4.5 µM 2,4-D + 9.8 µM 2-iP + 1.5 AC) allowed us to obtain the best value in terms of callus weight. After culturing on the PM1 (4.4 µM BA + 9.8 µM 2-iP) produced the highest numbers of somatic embryos and shoots. The explants on RM2 (0.5 µM NAA + 1.25 µM IBA + 3 g AC) showed the highest root numbers and root lengths, while the highest shoot length was achieved on RM3 (0.5 µM NAA + 0.5 µM IBA + 3 g AC). The Amri variety presented the best response among the three varieties in all parameters, followed by the Magdoul and Barhy varieties. In all the stages of micropropagation, the analysis of variance revealed highly significant variations among varieties and culture media, and a significant difference in the number of roots during the rooting stage. The results also showed non-significant differences in the interaction between varieties and culture media, except for shoot length in the rooting stage. The results also reveal the broad sense heritability ranging from low to high for the measured parameters. It can be concluded that the immature female inflorescences can be used as a productive explant source for successful date palm micropropagation using the SM1, MM1, PM1 and RM2 culture media. It can also be concluded that the success of date palm micropropagation not only depends on the concentrations of growth regulators, but also on their types.

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Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers

Cited by 33 publications

References 41 publications

Agro-morphological and genetic variability analysis in oat germplasms with special emphasis on food and feed

Agro-morphological and genetic variability analysis in oat germplasms with special emphasis on food and feed

Characterization of Cyclamen genotypes using morphological descriptors and DNA molecular markers in a multivariate analysis

In Vitro Propagation of Three Date Palm (Phoenix dactylifera L.) Varieties Using Immature Female Inflorescences

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