Genetic and Phenotypic Variation Among Faba Bean Landraces and Cultivars

Abstract

Landraces of crops can contain phenotypic and genotypic variability that can be exploited so that new lines can be developed. The phenotypic and genotypic variability among 11 Jordanian faba bean (Vicia faba L.) landraces and five imported cultivars were studied. Yield and 12 yield components and yield-related traits were evaluated at two locations. Analysis indicated significant variability among germplasm for all characters and among locations for 10 characters, in which final plant height, pods/plant, and seeds/pod were not significant. Genetic variability and relationships among germplasm were established using 12 single sequence repeat (SSR) primer pairs. Eleven primers were particularly efficient in amplification of banding patterns among germplasm. The overall polymorphic percentage for the 31 loci generated by 11 SSR markers was 84%. This was sufficient to estimate genetic variation among faba bean germplasm. The unweighted pair group method with arithmetic average ordered germplasm into three groups based on a Dice similarity coefficient of 0.71, range 0.7 to 0.89. Clustering based on phenotypical traits indicated five smaller groups that were consistent with the three larger SSR-based groups. These landraces can be used for future faba bean breeding programs and derivation of new plant lines.

The development of faba bean expressed sequence tag–simple sequence repeats (EST‐SSRs) and their validity in diversity analysis

“…Genomic DNA was extracted from fresh leaves of the 20 accessions using the cetyltrimethylammonium bromide method as described by Torres et al. (1993) with minor modifications (Abu‐Amer et al. 2011).…”

“…Because of limited availability of SSR markers in faba bean (Abu‐Amer et al. 2011, Gong et al 2011), the objectives of this study were to develop new faba bean EST‐SSR markers, to validate their ability to detect polymorphism among 13 different faba bean genotypes and to investigate their transferability across seven related Vicia spp.…”

Significance of genetic resources of cool season annual legumes: III: Locally cultivated and maintained crop landraces

“…A similar analysis with AFLP markers emphasized the separation of the Chinese local landraces of faba bean from the rest (Zong et al 2009), with a wide variability within the spring-sown ones . Similar studies using SSR markers in the local landraces of faba bean from Jordan (Abu-Amer et al 2010) and isozymes for those from Ethiopia (Serradilla et al 1993) also showed a local separation within the regions of Near East and North Africa. On the other hand, a wide variability was assessed among the local landraces in one country, such as Greece , Egypt (Mustafa 2007) or Ethiopia (Keneni et al 2005), opening a possibility that the geographic factors does not have to play a pivotal role on a lower level.…”