Genetic and Transmission Analysis of<i>Helicobacter pylori</i>Strains within a Family1

Raymond, Josette; Thiberge, Jean‐Michel; Chevalier, Catherine; Kalach, Nicolas; Bergeret, M.; Labigne, Agnès; Dauga, Catherine

doi:10.3201/eid1010.040042

Cited by 90 publications

(70 citation statements)

References 34 publications

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“…Although multiple strains have not been sequenced for all individuals in this study, we have shown that within-individual sequence diversity is low. This low within-individual genetic diversity, combined with the observation of dominant strains (Raymond et al 2004), provides support for the single-strain assumption used in this study. Indeed, several recent studies that have sequenced multiple strains found substantially lower sequence divergences within individuals (Israel et al 2001, Raymond et al 2004, Kraft et al 2006, suggesting a shared common ancestor for multiple strains within individuals.…”

Section: Discussionsupporting

confidence: 48%

“…Multiple strain data generated in this study (mutY: 3 individuals, 66 pairwise comparisons; ureI: 2 individuals, 28 pairwise comparisons) indicated low average within-individual genetic distances (mutY: 0.006 6 0.01, n ¼ 19; ureI: 0.014 6 0.013, n ¼ 12). Furthermore, Raymond et al (2004) sequenced 20 cultures for each of 6 individuals and detected a maximum of four strains per individual, where each individual appeared to be infected by a dominant strain with .70% occurrence. Mean average ureC genetic distance within individuals was low (0.023 6 0.004, n ¼ 5).…”

Section: Resultsmentioning

confidence: 99%

“…PCR products were cloned into the pTZ57R vector using the InsT/ Aclone PCR product cloning kit (Fermentas). These data were combined with published multiple strain data (Raymond et al 2004) to calculate the degree of within-individual sequence diversity and thus to determine whether the single-strain assumption was acceptable, given the intended aims of this study.…”

Section: Methodsmentioning

confidence: 99%

“…1 spread of H. pylori. Previous transmission studies of H. pylori have been conducted in low-incidence study populations related to their socioeconomic status (Rothenbacher et al 1999;Tindberg et al 2001), with small within-family sample sizes (Rothenbacher et al 1999;Samir et al 2004;Raymond et al 2004), and using low-resolution methods for the inference of transmission (Rothenbacher et al 1999;Tindberg et al 2001;Samir et al 2004). These characteristics should be further explored.…”

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confidence: 99%

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A Population Genetics Pedigree Perspective on the Transmission of Helicobacter pylori

et al. 2006

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The inference of transmission pathways for medicinally important bacteria is important to our understanding of pathogens. Here we report analyses of transmission in Helicobacter pylori, a major carcinogen. Our study is novel in that the focal community comprises detailed family pedigrees and has a high prevalence of H. pylori. To infer transmission, we performed high-resolution analyses of nucleotide sequences for three genes and accounted for the occurrence of mutation and recombination through the use of simulation modeling. Our results demonstrate that transmission has a strong nonfamilial component potentially the result of a large proportion of infections derived from the community. These results are interesting from both a medical and an evolutionary standpoint. First, efficient control measures and beliefs about the sources of H. pylori infection should be reevaluated. Evolutionarily, our results contradict the hypothesis of strict vertical transmission, presented as an explanation for the strong correlation between human population history and H. pylori diversity. Thus the paradox of persistent phylogenetic structure, despite a permissive mode of transmission and high recombination rates, must be solved elsewhere. Here we consider the potential for recombination events to maintain genetic structure in light of horizontal transmission.

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Section: Discussionsupporting

confidence: 48%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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A Population Genetics Pedigree Perspective on the Transmission of Helicobacter pylori

et al. 2006

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“…Transmission within families occurs for many indigenous organisms (14) and can resemble inheritance patterns (55). Furthermore, whether a microbe is acquired from a family member (and so is preadapted to the next host) or from a genetically unrelated stranger could modify the risk of a disease that may present clinically decades later (46).…”

Section: Biological Considerationsmentioning

confidence: 99%

Understanding Microbe-Induced Cancers

Blaser

2008

Cancer Prevention Research

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Microbes are important causes of human cancers, and our estimation of their significance continues to grow as cancer biology is better dissected. A classification system is proposed that highlights common and proposed microbe-induced pathways toward oncogenesis, with an emphasis on types of targeted cells and host-microbial interactions. The central principles that underlie oncogenesis induced by the many diverse microbes and the major mechanisms involved are outlined. The phenomenon of microbe-induced cancers raises a number of important biological questions, the solving of which may inform other fields, including aging and degenerative disorders. Finally, our challenge for the future is to better understand the steps in microbe-induced cancers to optimize both prevention and therapy.

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Updated joint ESPGHAN/NASPGHAN guidelines for management of Helicobacter pylori infection in children and adolescents (2023)

Homan,

Jones,

Bontems

et al. 2024

J. pediatr. gastroenterol. nutr.

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BackgroundEvolving epidemiological data and increasing antibiotic resistance mandate an update of the European and North American Societies of Pediatric Gastroenterology, Hepatology and Nutrition guidelines.MethodsCertainty of evidence and strength of recommendations were rated by experts according to the Grading of Recommendation Assessment, Development, and Evaluation approach. PICO (patient population, intervention, comparator, and outcome) questions were developed and voted on by the group. Recommendations were formulated using the Evidence to Decision framework.ResultsThe current literature supports many of the previous recommendations and several new recommendations. Invasive testing with strain antimicrobial susceptibility analysis is recommended for the diagnosis and selection of eradication therapy for H. pylori infection. Molecular methods are acceptable for detection of infection and of antibiotic resistance in gastric biopsy specimens. Reliable, noninvasive tests can be used as a screening method for children with history of gastric cancer in a first‐degree relative. When investigating causes of chronic immune thrombocytopenic purpura, testing for H. pylori is no longer recommended. When investigating other diseases such as inflammatory bowel disease, celiac disease, or eosinophilic esophagitis, specific diagnostic biopsies for H. pylori infection are not indicated. However, if H. pylori is an incidental finding, treatment may be considered after discussing the risks and benefits. Treatment should be based on antibiotic antimicrobial susceptibility testing and, if unavailable, regimens containing clarithromycin should be avoided.ConclusionsDue to decreasing prevalence of infection, increasing challenges with antibiotic resistance, and emerging evidence regarding complications of infection, clinicians must be aware of these recommended changes to appropriately manage H. pylori infection and its clinical sequelae in children.

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Genetic and Transmission Analysis ofHelicobacter pyloriStrains within a Family1

Abstract: Point mutations, intragenic recombination, and introduction of foreign alleles enhanced strain diversity within the family.

Cited by 90 publications

References 34 publications

A Population Genetics Pedigree Perspective on the Transmission of Helicobacter pylori

A Population Genetics Pedigree Perspective on the Transmission of Helicobacter pylori

Understanding Microbe-Induced Cancers

Updated joint ESPGHAN/NASPGHAN guidelines for management of Helicobacter pylori infection in children and adolescents (2023)

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