Genetic background and production periods shape the microRNA profiles of the gut in laying hens

Ponsuksili, Siriluck; Hadlich, Frieder; Reyer, Henry; Oster, Michael; Trakooljul, Nares; Iqbal, Muhammad Arsalan; Sommerfeld, Vera; Rodehutscord, M.; Wimmers, Klaus

doi:10.1016/j.ygeno.2021.04.018

Cited by 7 publications

(16 citation statements)

References 51 publications

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“…LSL hens seem to have a rather adaptive immunological phenotype, while LB hens have a pronounced innate immunological phenotype ( Hofmann et al, 2021 ). These findings were confirmed at the molecular level of miRNAs and their target transcripts, which show that in LB, miRNA target transcripts were enriched for metabolic pathways, whereas in LSL, miRNA targets were more abundant for immune and inflammatory processes ( Ponsuksili et al, 2021 ). The microbial community differed significantly between strains and gut sections.…”

Section: Introductionmentioning

confidence: 64%

“…In our previous studies, animals of 2 laying hen strains (Lohmann Brown-Classic [ LB ], Lohmann LSL-Classic [ LSL ]) were studied over 5 developmental stages. These studies focused on individual data sets at various biological levels, such as physiological, endocrine, metabolic, immune, and transcriptomic ( Sommerfeld et al, 2020a ; Gonzalez-Uarquin et al, 2021 ; Omotoso et al, 2021 ; Ponsuksili et al, 2021 ; Schmucker et al, 2021 ). All data sets were obtained with the same animals.…”

Section: Introductionmentioning

confidence: 99%

“…The microbial community differed significantly between strains and gut sections. The marked changes in nutrients, especially Ca, from pre-laying to laying between 16 and 24 wk of age, resulted in the most striking difference not only in metabolic, endocrine, immunological and transcriptomic traits, but also in the gut's microbial community ( Sommerfeld et al, 2020b ; Gonzalez-Uarquin et al, 2021 ; Omotoso et al, 2021 ; Ponsuksili et al, 2021 ; Schmucker et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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The dynamics of molecular, immune and physiological features of the host and the gut microbiome, and their interactions before and after onset of laying in two hen strains

et al. 2023

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Section: Introductionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The dynamics of molecular, immune and physiological features of the host and the gut microbiome, and their interactions before and after onset of laying in two hen strains

et al. 2023

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“…In chicken primordial germ cells, gga-miR-29b was found to downregulate the expression of DNMT3B, which provides more knowledge to explain the role of DNMT genes regulation in embryogenesis (19). In the jejunal mucosa of laying hens with different genetic backgrounds, the potential target genes of miR-29b-3p were enriched in energy pathways at different stages of production periods (20).…”

Section: Introductionmentioning

confidence: 90%

Role of gga-miR-29b-3p in suppressing the proliferation, invasion and migration of MSB1 Marek’s disease tumor cells by the targeting of the DNMT3B gene

Han¹,

Lian²,

Ren³

et al. 2022

Ann Transl Med

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Background: Marek's disease (MD), a class II infectious, lymphoproliferative disease that mainly afflicts poultry, has been shown to cause wasting, limb paralysis, and often acute death. It is a neoplastic disease caused by a cell-binding herpesvirus that leads to the formation of tumors in various organs and tissues. Our previous reports have found that the microRNA, gga-miR-29b-3p, showed abnormal expression in MD lymphoma. However, it remains unknown whether gga-miR-29b-3p affects MD tumorigenesis. Methods:The MD tumor cell line MSB1 was chosen to analyze the characteristics of gga-miR-29b-3p in tumors. Cell proliferation and migration were assessed by Cell Counting Kit-8 (CCK-8) and Transwell, respectively, and cell apoptosis and cycle were analyzed via fluorescent staining and flow cytometry, respectively. The regulation between gga-miR-29b-3p and its potential target genes was verified by dual luciferase results and loss-of-function assays. The effect of target genes was verified by examining the degree of RNA interference on MSB1 cells.Results: Analysis revealed that gga-miR-29b-3p impaired the proliferation of the MSB1 MD tumor cell line, induced apoptosis without obvious effects on the cell cycle, and suppressed the expression of the invasion-associated MMP2 and MMP9 genes. It was concluded that DNMT3B is the direct target of gga-miR-29b-3p. As expected, the effects of DNMT3B knockdown with small interfering RNA (siRNA) on MSB1 cell proliferation, apoptosis, and cycle were associated with gga-miR-29b-3p overexpression.Moreover, BCL2 and BCL2L1 were downregulated and TNFSF10 was upregulated in both the gga-miR-29b-3p overexpression and DNMT3B knockdown groups. The expression levels of invasion-related genes were decreased post-DNMT3B knockdown. In both the gga-miR-29b-3p overexpression and DNMT3B knockdown conditions, a decrease in MEQ oncogene expression in MD virus was observed.Conclusions: Overall, gga-miR-29b-3p was demonstrated to have a suppressive effect in MD lymphoma progression via the targeting of the DNMT3B gene. Gga-miR-29b-3p overexpression and DNMT3B knockdown inhibited MSB1 cell proliferation through suppressing the pro-apoptotic gene expression and elevating the anti-apoptotic gene expression in the apoptosis pathway. Our study provides a theoretical basis for targeted treatment of MD.

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“…Both used strains (Lohmann Brown and Lohmann Selected Leghorn) are commercial important and selected for egg production [13][14][15]. Despite their similarities in egg production previous studies have shown differences between them concerning body weight, gene expression and phytate degradation in the context of the productive life span and changes in dietary Ca and P concentrations [10,16,17]. These results suggest differences in the strains reaction to dietary changes, which will be analysed in this work.…”

Section: Introductionmentioning

confidence: 96%

The impact of dietary calcium and phosphorus on mitochondrial-linked gene expression in five tissues of laying hens

Dreyling

Hasselmann

2022

PLoS ONE

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Mitochondria and the energy metabolism are linked to both, the availability of Ca and P to provide the eukaryotic cell with energy. Both minerals are commonly used supplements in the feed of laying hens but little is known about the relationship between the feed content, energy metabolism and genetic background. In this study, we provide a large-scaled gene expression analysis of 31 mitochondrial and nuclear encoded genes in 80 laying hens in the context of dietary P and Ca concentrations. The setup included five tissues and gene expression was analysed under four different diets of recommended and reduced Ca and P concentrations. Our study shows, that mitochondrial gene expression is reacting to a reduction in P and that an imbalance of the nutrients has a higher impact than a combined reduction. The results suggest, that both strains (Lohmann Brown and Lohmann Selected Leghorn) react in a similar way to the changes and that a reduction of both nutrients might be possible without crucial influence on the animals’ health or gene expression.

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Genetic background and production periods shape the microRNA profiles of the gut in laying hens

Cited by 7 publications

References 51 publications

The dynamics of molecular, immune and physiological features of the host and the gut microbiome, and their interactions before and after onset of laying in two hen strains

The dynamics of molecular, immune and physiological features of the host and the gut microbiome, and their interactions before and after onset of laying in two hen strains

Role of gga-miR-29b-3p in suppressing the proliferation, invasion and migration of MSB1 Marek’s disease tumor cells by the targeting of the DNMT3B gene

The impact of dietary calcium and phosphorus on mitochondrial-linked gene expression in five tissues of laying hens

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