Genetic Characterization and Mapping of Anthracnose Resistance of Common Bean Landrace Cultivar Corinthiano

Sousa, Lorenna Lopes de; Gonçalves, Aline O.; Gonçalves‐Vidigal, Maria Celeste; Lacanallo, Giselly Figueiredo; Fernandez, Andrea C.; Awale, Halima E.; Kelly, James D.

doi:10.2135/cropsci2014.09.0604

Cited by 44 publications

(51 citation statements)

References 76 publications

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“…Further, Co-u mapped on Pv02 [11], and Co-13 and Co-17 mapped onto Pv03 [5,12]. The linkage group Pv04 includes six genes, namely: Co-3 , Co-9 , Co-y , Co-z , Co-10 and Co-15 [10,13–17]. Additionally, Co-5 , Co-6 and Co-v were located on the Pv07 [18,19], Co-4 on the Pv08 linkage group and Co-2 on the Pv11 [16,20,21].…”

Section: Introductionmentioning

confidence: 99%

Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L.)

Chen

Wang

et al. 2017

PLoS ONE

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Anthracnose is a destructive disease of the common bean (Phaseolus vulgaris L.). The Andean cultivar Hongyundou has been demonstrated to possess strong resistance to anthracnose race 81. To study the genetics of this resistance, the Hongyundou cultivar was crossed with a susceptible genotype Jingdou. Segregation of resistance for race 81 was assessed in the F2 population and F2:3 lines under controlled conditions. Results indicate that Hongyundou carries a single dominant gene for anthracnose resistance. An allele test by crossing Hongyundou with another resistant cultivar revealed that the resistance gene is in the Co-1 locus (therefore named Co-1HY). The physical distance between this locus and the two flanking markers was 46 kb, and this region included four candidate genes, namely, Phvul.001G243500, Phvul.001G243600, Phvul.001G243700 and Phvul.001G243800. These candidate genes encoded serine/threonine-protein kinases. Expression analysis of the four candidate genes in the resistant and susceptible cultivars under control condition and inoculated treatment revealed that all the four candidate genes are expressed at significantly higher levels in the resistant genotype than in susceptible genotype. Phvul.001G243600 and Phvul.001G243700 are expressed nearly 15-fold and 90-fold higher in the resistant genotype than in the susceptible parent before inoculation, respectively. Four candidate genes will provide useful information for further research into the resistance mechanism of anthracnose in common bean. The closely linked flanking markers identified here may be useful for transferring the resistance allele Co-1HY from Hongyundou to elite anthracnose susceptible common bean lines.

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Section: Introductionmentioning

confidence: 99%

Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L.)

Chen

Wang

et al. 2017

PLoS ONE

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“…Dominant epistatic inheritance has usually been observed in the presence of several resistance genes, although a complementary mode of action between two resistance genes was described in some cases (Campa et al, 2011, 2014; Muhalet et al, 1981). Most of the anthracnose resistance loci reported have also been located in the genetic map, and markers tagging these genes are available: genes Co‐1 , Co‐x , and Co‐w were mapped on LG Pv01 (Rodríguez‐Suárez et al, 2007; Geffroy et al, 2008); Co‐u on Pv02 (Geffroy et al, 2008); Co‐13 on Pv03 (Gonçalves‐Vidigal et al, 2009); Co‐3 , Co‐9 , Co‐y , Co‐z Co‐10 , and Co‐15 on Pv04 (Geffroy et al, 1999; Alzate‐Marin et al, 2003; Méndez‐Vigo et al, 2005; Rodríguez‐Suárez et al, 2007, 2008; Sousa et al, 2013); Co‐5 , Co‐6 , and Co‐v on Pv07 (Geffroy, 1997; Campa et al, 2009); Co‐4 on Pv08 (Melotto et al, 2004; Rodríguez‐Suárez et al, 2007); CoPv09 on Pv09 (Campa et al, 2014); and Co‐2 on Pv11 (Adam‐Blondon et al, 1994). Allelic variants of Co‐1 , Co‐3 , and Co‐4 have been proposed from the differences observed in the resistance spectrum between different bean genotypes.…”

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confidence: 99%

Identification of a New Chromosomal Region Involved in the Genetic Control of Resistance to Anthracnose in Common Bean

2015

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Anthracnose caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.-Scrib. is a major disease affecting common bean (Phaseolus vulgaris L.) crops worldwide. Response to five C. lindemuthianum isolates, classified as races 3, 6, 7, 38, and 73, were analyzed in 156 F 2:3 families derived from the cross between line SEL1308 and cultivar Michigan Dark Red Kidney (MDRK). SEL1308 was resistant to all five races, while MDRK was susceptible to all except for race 73. Segregation ratio for response to races 3 and 7 indicated that single dominant genes were responsible for the resistance reaction to each race. Recombination between both race-specific genes was observed and no linkage was found with any of the molecular markers tagging Co-genes or clusters previously described. Linkage analyses allowed the location of both genes at the beginning of linkage group (LG) Pv03, a region tentatively named as Co-17. Segregation ratio for response to races 6 and 38 indicated that two dominant and independent genes conferred resistance to these races. Contingency tests and subpopulation analyses suggested the implication of one region on LG Pv08, corresponding to the Co-4 cluster, and the Co-17 region. For reaction to race 73, the most likely scenario was that two dominant and independent genes conferred resistance: Co-1 in MDRK and Co-4 2 in SEL1308. Results indicated that, in addition to Co-4 2 , SEL1308 carries resistance genes located at the beginning of LG Pv03, in which no anthracnose resistance genes were previously mapped. In silico analysis revealed the presence of seven genes codifying typical resistance proteins (R-proteins) in the underlying physical position of the Co-17 region.

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“…The PhaseolusGenes marker database has become an essential tool for cataloging bean marker diversity so that the breeding community can utilize marker information from multiple sources to facilitate MAS (Gonçalves-Vidigal et al, 2011Reinprecht et al, 2013;Oblessuc et al, 2013Oblessuc et al, , 2015Aranda et al, 2014;Keller et al, 2015;Sousa et al, 2015;Coimbra-Gonçalves et al, 2016;Perseguini et al, 2016). Because genomic resequencing of breeding parents is today a more routine practice with the lowered cost of sequencing, the PhaseolusGenes database should evolve from a marker database into a sequence database, which includes not only the current markers but also the increasingly large number (> 2,000) of genotypes that have been sequenced, including the 16 parental genotypes of the ABC projects (T. Miller and P. Gepts, unpubl.…”

Section: Discussionmentioning

confidence: 99%

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

Miller¹,

Gepts²,

Kimno³

et al. 2018

Afr. J. Biotechnol.

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Common bean (Phaseolus vulgaris) is a rich source of protein, vitamins, and micronutrients and is an important crop for food security throughout Latin America, Asia, and Africa. Among tropical and subtropical regions of the world, where the majority of beans are grown, yield losses due to the pathogenic fungus Pseudocercospora griseola, causing angular leaf spot (ALS), can be as high as 80%. The strategic use of marker-assisted selection (MAS) to pyramid multiple resistance genes into a single genetic background with preferred morphological and cooking characteristics is being implemented by six research groups throughout East Africa that make up the African Bean Consortium (ABC). Identifying unique markers that are polymorphic among multiple parents is a major source of marker attrition. In this study, an illustration of how 22 DNA sequences physically linked to the Phg-2 ALS resistance locus were identified using the PhaseolusGenes marker database and checked for amplification and polymorphism among 16 ABC breeding parents are given. Only three polymorphic markers could be identified following this procedure; one (g796), showed a polymorphism present only in the ALS resistance donor, Mexico 54. The PCR protocol developed to identify the g796 polymorphism was validated among five laboratories. Furthermore, co-segregation analysis of the marker and ALS resistance phenotype in a population of 100 F 2 individuals from the cross between French bean (that is, green bean) genotype Amy and ALS resistance donor Mexico 54 showed the marker is genetically linked (3 cM) to the Phg-2 locus, in addition to being physically linked. This study suggests that in the near future, genetic resequencing data of diverse common bean accessions, compiled within an easily accessible database, will facilitate identification of markers for MAS, marker/trait association, and candidate gene identification.

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Genetic Characterization and Mapping of Anthracnose Resistance of Common Bean Landrace Cultivar Corinthiano

Cited by 44 publications

References 76 publications

Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L.)

Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L.)

Identification of a New Chromosomal Region Involved in the Genetic Control of Resistance to Anthracnose in Common Bean

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

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