Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia

Abstract

Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.

Phylogenetic comparison of porcine circovirus type 2 (PCV2) and porcine reproductive respiratory syndrome virus (PRRSV) strains detected in domestic pigs until 2008 and in 2012 in Croatia

“…No isolates from group PCV2a were found in 2012 indicating that this viral group has spontaneously decreased in its spread during the last decade or has been eliminated from the tested farms as a result of vaccination. Similar findings were reported and recognised in countries neighbouring Croatia, such as Slovenia [25], Hungary [26], Austria [27], Serbia [28,29], and Italy [30]. Vaccination against PCV2 infection in Croatia was introduced rather late, in 2010.…”

Phylogenetic and Phylogeographic Analyses of Porcine Circovirus Type 2 Among Pig Farms in Vietnam

“…Each genetic cluster was found at more than one site and indicates the co‐circulation of viruses among pig farms. In recent years, PCV2 viruses bearing a lysine extension at the C‐terminus of the capsid protein have been reported in several countries, including Germany (Knell et al., ), Indonesia (Manokaran et al., ), China (Wang et al., ; Guo et al., ) and Serbia (Savic et al., ). In addition, the PCV2b genotype with the capsid protein mutation has been confirmed to display increased virulence in vivo compared to classical PCV2a and PCV2b genotypes (Guo et al., ).…”

Survey Of Infectious Agents Associated With Porcine Respiratory Disease Complex (PRDC) In Serbian Swine Herds Using Polymerase Chain Reaction (PCR) Detection

“…The lysed samples were processed with a DNA extraction kit (QIA extraction DNA kit, Qiagen) according to the manufacturer's instructions. All samples were tested by PCR using HotStarTaq Master Mix Kit (Qiagen), and primers described for DNA detection of PCV2 [11], Mhp [12], Pm [13], App [14], Hps [15], Ssuis [16] and Apyo [17]. After suspension, the samples were processed with a RNA extraction kit (Qiagen -Rneasy MiniKit) according to th e manufacturer's instructions.…”