Genetic characterization and phylogenetic analysis of Newcastle disease virus from China

Liu, Yaqing; Sun, Chengxi; Chi, Miaomiao; Wen, Hao; Zhao, Li; Song, Yue; Liu, Na; Wang, Zhiyu

doi:10.1016/j.meegid.2019.103958

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…HN glycoprotein has activities such as hemagglutination (HA), NA and stimulates F protein activity (Soltani et al., 2019 ). The binding of the HN protein to the sialic acid receptor initiates protein F action, which leads to the fusion of the virus membrane and the host cell membrane (Liu et al., 2019b ). NDV virus isolates have a high mutation potential that enables several genotypes of viruses to grow at the same time.…”

Section: Introductionmentioning

confidence: 99%

Molecular evaluation and genetic characterisation of Newcastle disease virus's haemagglutinin‐neuraminidase protein isolated from broiler chickens in Iran

Shafaati

Ghorbani

Mahmodi

et al. 2021

Veterinary Medicine & Sci

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Background: Newcastle disease (ND) virus (NDV) is one of the major pathogens in poultry farms that causes severe economic damages to the poultry industry, especially broiler chicken and turkey farms. Despite the endemicity of ND and its many epidemics in the country, the nature of the Iranian strain of the Newcastle virus is still largely unknown. This study aimed to characterise and evaluate NDV isolates obtained from commercial poultry farms in Iran in 2019 through haemagglutinin-neuraminidase (HN) gene sequencing.Method: HN gene of each NDV isolate was amplified and sequenced using specific primers followed by phylogenetic analysis of full length of HN gene open reading frame and amino acid (aa) sequence of HN.Results: Phylogenetic analysis of the HN gene showed that the virus is very closely related to genotypes VII and III. Analysis of HN gene nucleotide sequences showed that all isolates encode proteins with a length of 571 aa. Conclusion:Results of the present study are useful for a better understanding of molecular epidemiology of indigenous NDV strains and determining important molecular differences between fields and commonly used vaccine strains related to main immunogenic proteins.

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Section: Introductionmentioning

confidence: 99%

Molecular evaluation and genetic characterisation of Newcastle disease virus's haemagglutinin‐neuraminidase protein isolated from broiler chickens in Iran

Shafaati

Ghorbani

Mahmodi

et al. 2021

Veterinary Medicine & Sci

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“…These genotype-mismatched vaccines can provide some protection against the challenge of virulent NDVs; however, they cannot protect the chickens from infection, and cannot effectively reduce the viral shedding [ 25 , 26 ]. The class II genotype VII NDV is a major genotype prevalent in the Middle East, Africa, and Asia regions; however, the vaccines used in these places are usually genotype Ⅱ La Sota and B1 strains, which are not the same genotype with prevalent strains [ 27 , 28 , 29 ]. In this study, in order to generate a thermostable genotype VII-matched vaccine candidate, a thermostable genotype VIII HR09 strain was used as the backbone, and two chimeric genotype VII-matched vaccine candidates were constructed to fulfill the need for a vaccine for genotype VII.…”

Section: Discussionmentioning

confidence: 99%

Construction of Novel Thermostable Chimeric Vaccine Candidates for Genotype VII Newcastle Disease Virus

Cao

Ruan

et al. 2022

Viruses

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Genotype VII Newcastle Disease Virus (NDV) has caused a pandemic in many countries and usually causes fatal consequences in infected chickens. Although current commercial attenuated NDV vaccines can provide an ideal protection against genotype VII NDV, they cannot completely prevent the infection and viral shedding, and the genotype of some vaccine strains cannot match with the prevalent strain. In this study, in order to construct a thermostable and genotype VII-matched live attenuated vaccine, we used a thermostable genotype VIII virulent HR09 strain as the backbone and replaced its F gene with that of the genotype VII DT-2014 strain. Meanwhile, the cleavage site of F gene of DT-2014 was mutated to that of class I F protein and avirulent class II F protein, respectively. The results showed that the two chimeric viruses, designated rcHR09-CI and rcHR09-CII, shared a similar growth kinetics and thermostability with their parental HR09 strain. Mean death time (MDT) and intracerebral pathogenicity index (ICPI) tests showed that the two chimeric viruses were highly attenuated. Though both chimeric NDVs and La Sota vaccine strain could provide complete protection to immunized chickens against the challenge of virulent genotype VII ZJ1 strain, the two chimeric NDVs could induce a higher level of antibody response against ZJ1 strain and could significantly reduce the viral shedding compared with La Sota vaccine strain. In conclusion, our study constructed two chimeric thermostable genotype VII-matched NDV vaccine candidates, which provided complete protection against the challenge of virulent genotype VII NDV.

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“…[5]. В течение следующих нескольких лет первая из них проникла в Малайзию [6], Китай [7], ряд стран Южной Африки [8] и Россию (NDV/chicken/ Kaliningrad/184/2013). Второй субгенотип был выявлен в Пакистане [9], Индии [10], Израиле [11], Ливии [12], Турции, Грузии и Болгарии [13].…”

Section: оригинальные статьи | болезни птиц Original Articles | Avianunclassified

Effectiveness of vaccines produced by the Federal State-Financed Institution “ARRIAH” against topical genotype VII Newcastle disease viruses

Фролов

Мороз

Чвала

et al. 2021

Veterinariâ segodnâ

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In 2019, the situation regarding Newcastle disease in the Russian Federation worsened radically due to the spread of NDV subgenotype VII-L throughout the country from the Primorsky Krai to the Kursk Oblast. As a result, 17 infected settlements with backyard farms where unvaccinated poultry was kept were registered. In this study, immunogenicity of the vaccines produced by the FGBI “ARRIAH”, as well as the effectiveness of various vaccination schedules to prevent genotype VII NDVs, relevant for the Russian Federation, was studied. It is known that the currently circulating ND agent is significantly more virulent compared to the viruses isolated in previous years, and it is able to bypass the immunity provided by live vaccines. Test results demonstrated that the vaccines against genotype VII NDVs produced by the FGBI “ARRIAH” are highly immunogenic, which allows to effectively prevent the disease when using them as part of a standard vaccination schedule. A 2-dose vaccination schedule using live vaccine from the La Sota strain as well as the “complete” vaccination schedule using inactivated vaccines provides immunity in 100% of chicks. The use of live vaccines in a single- and double-dose vaccination schedules prevents mortality and clinical disease in poultry, but does not prevent virus replication, while the addition of an inactivated vaccine to the immunization schedule does prevent the replication of the virulent virus. Thus, the use of domestically produced live and inactivated vaccines, primarily the ones containing the La Sota strain, with the following control of the immunity level and booster vaccination, if required, is the main tool for the disease control.

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Genetic characterization and phylogenetic analysis of Newcastle disease virus from China

Cited by 8 publications

References 32 publications

Molecular evaluation and genetic characterisation of Newcastle disease virus's haemagglutinin‐neuraminidase protein isolated from broiler chickens in Iran

Molecular evaluation and genetic characterisation of Newcastle disease virus's haemagglutinin‐neuraminidase protein isolated from broiler chickens in Iran

Construction of Novel Thermostable Chimeric Vaccine Candidates for Genotype VII Newcastle Disease Virus

Effectiveness of vaccines produced by the Federal State-Financed Institution “ARRIAH” against topical genotype VII Newcastle disease viruses

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