Genetic characterization of streptomycin-resistant and-dependent mutants of Escherichia coli K12

Sánchez-Anzaldo, F. J.; Bastarrachea, Fernando

doi:10.1007/bf00270518

Cited by 12 publications

(5 citation statements)

References 31 publications

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“…Some Str r mutations in strains with pVB11, pJI3, pUOA2E1, or pUOA2E2 had no effect on tetracycline MICs. In contrast, for two Str d strains known as strM (29), the MICs specified by Tet(M) and Tet(O) plasmids were reduced; e.g., the MIC for CGSC6983(pVB11) a For the biosynthetic pathway in which miaA and miaB genes are involved, see Fig. 1.…”

Section: Mics Specified By Tet(m) and Tet(o)mentioning

confidence: 99%

Host Mutations ( miaA and rpsL ) Reduce Tetracycline Resistance Mediated by Tet(O) and Tet(M)

Taylor

Trieber²,

Trescher³

et al. 1998

Antimicrob Agents Chemother

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The effects of mutations in host genes on tetracycline resistance mediated by the Tet(O) and Tet(M) ribosomal protection proteins, which originated in Campylobacter spp. andStreptococcus spp., respectively, were investigated by using mutants of Salmonella typhimuriumand Escherichia coli. The miaA,miaB, and miaAB double mutants of S. typhimurium specify enzymes for tRNA modification at the adenosine at position 37, adjacent to the anticodon in tRNA. InS. typhimurium, this involves biosynthesis ofN 6-(4-hydroxyisopentenyl)-2-methylthioadenosine (ms2io6A). The miaA mutation reduced the level of tetracycline resistance mediated by both Tet(O) and Tet(M), but the latter showed a greater effect, which was ascribed to the isopentenyl (i6) group or to a combination of the methylthioadenosine (ms2) and i6 groups but not to the ms2 group alone (specified by miaB). In addition, mutations in E. coli rpsL genes, generating both streptomycin-resistant and streptomycin-dependent strains, were also shown to reduce the level of tetracycline resistance mediated by Tet(O) and Tet(M). The single-site amino acid substitutions present in the rpsL mutations were pleiotropic in their effects on tetracycline MICs. These mutants affect translational accuracy and kinetics and suggest that Tet(O) and Tet(M) binding to the ribosome may be reduced or slowed in theE. coli rpsL mutants in which the S12 protein is altered. Data from both the miaA and rpsL mutant studies indicate a possible link between stability of the aminoacyl-tRNA in the ribosomal acceptor site and tetracycline resistance mediated by the ribosomal protection proteins.

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Section: Mics Specified By Tet(m) and Tet(o)mentioning

confidence: 99%

Host Mutations ( miaA and rpsL ) Reduce Tetracycline Resistance Mediated by Tet(O) and Tet(M)

Taylor

Trieber²,

Trescher³

et al. 1998

Antimicrob Agents Chemother

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“…It is only known by the effect of a mutation which inhibits ochre suppression (199). It maps in the same region as the strM gene, but mutations in that gene are known to increase the accuracy of translation in streptomycin-dependent strains (176,199).…”

mentioning

confidence: 99%

“…The function of this gene is unknown. Mutations in strM were detected by their effect of restricting streptomycin-mediated suppression of amber mutations in streptomycin-dependent strains and are only known by this quality (176). Thus, the strM gene appears somehow to affect translational accuracy.…”

mentioning

confidence: 99%

Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli.

Eggertsson¹,

Söll²

1988

Microbiological Reviews

129

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“…The asuFJ2 mutation has no discernible effect on tRNA amounts or structure and so may be a release factor mutation. The strM mutation maps near asuF and may be allelic with it because the phenotype of both are consistent with increased translation accuracy in mutant strains (35).…”

Section: Discussionmentioning

confidence: 98%

Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine

Sullivan¹,

Cannon²,

Webb³

et al. 1985

J Bacteriol

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Mutations in three Escherichia coli K-12 genes were isolated that reduce the efficiency of the lysine-inserting nonsense suppressor supL. These antisuppressor mutations asuD, asuE, and asuF map at 61.9, 25.3, and 76.3 min, respectively, on the E. coli chromosome. Biochemical and genetic analysis of the mutant strains revealed the reason for the antisuppressor phenotype for two of these genes. The activity of lysyl-tRNA synthetase was reduced in strains with asuD mutations. The modification of 5-methylaminomethyl-2-thiouridine, the wobble base of tRNALYS, was impaired in asuE mutant strains, presumably at the 2-thiolation step. CGSCa J. Davies J. Miller CGSC CGSC CGSC J. Miller J. Miller J. Miller CGSC CGSC

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Genetic characterization of streptomycin-resistant and-dependent mutants of Escherichia coli K12

Cited by 12 publications

References 31 publications

Host Mutations ( miaA and rpsL ) Reduce Tetracycline Resistance Mediated by Tet(O) and Tet(M)

Host Mutations ( miaA and rpsL ) Reduce Tetracycline Resistance Mediated by Tet(O) and Tet(M)

Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli.

Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine

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