Genetic damage and lipid peroxidation in workers occupationally exposed to organic bentonite particles

Huang, Yu-Wen; Zhang, Meibian; Zou, Hua; Li, Xiaoxue; Xing, Mingluan; Fang, Xinglin; He, Jiliang

doi:10.1016/j.mrgentox.2012.10.006

Cited by 27 publications

(13 citation statements)

References 28 publications

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“…Limited exposure studies along the forecasted ONC lifecycle indicate the potential for airborne respirable particle release with maximum levels reported at 12.73 and 39.2 mg/m 3 during ONC synthesis 24 and 131000 particles/cm 3 released from ONC composite materials. 10 However, no study has investigated in vivo pulmonary exposure to ONCs or their incinerated end-of-life byproducts.…”

Section: Resultsmentioning

confidence: 99%

Short-Term Pulmonary Toxicity Assessment of Pre- and Post-incinerated Organomodified Nanoclay in Mice

et al. 2018

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Organomodified nanoclays (ONCs) are increasingly used as filler materials to improve nanocomposite strength, wettability, flammability, and durability. However, pulmonary risks associated with exposure along their chemical lifecycle are unknown. This study's objective was to compare pre- and post-incinerated forms of uncoated and organomodified nanoclays for potential pulmonary inflammation, toxicity, and systemic blood response. Mice were exposed via aspiration to low (30 μg) and high (300 μg) doses of preincinerated uncoated montmorillonite nanoclay (CloisNa), ONC (Clois30B), their respective incinerated forms (I-CloisNa and I-Clois30B), and crystalline silica (CS). Lung and blood tissues were collected at days 1, 7, and 28 to compare toxicity and inflammation indices. Well-dispersed CloisNa caused a robust inflammatory response characterized by neutrophils, macrophages, and particle-laden granulomas. Alternatively, Clois30B, I-Clois30B, and CS high-dose exposures elicited a low grade, persistent inflammatory response. High-dose Clois30B exposure exhibited moderate increases in lung damage markers and a delayed macrophage recruitment cytokine signature peaking at day 7 followed by a fibrotic tissue signature at day 28, similar to CloisNa. I-CloisNa exhibited acute, transient inflammation with quick recovery. Conversely, high-dose I-Clois30B caused a weak initial inflammatory signal but showed comparable pro-inflammatory signaling to CS at day 28. The data demonstrate that ONC pulmonary toxicity and inflammatory potential relies on coating presence and incineration status in that coated and incinerated nanoclay exhibited less inflammation and granuloma formation than pristine montmorillonite. High doses of both pre- and post-incinerated ONC, with different surface morphologies, may harbor potential pulmonary health hazards over long-term occupational exposures.

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Section: Resultsmentioning

confidence: 99%

Short-Term Pulmonary Toxicity Assessment of Pre- and Post-incinerated Organomodified Nanoclay in Mice

et al. 2018

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“…Two main hypotheses could be advanced to explain these results: a decrease in ROS production and/or an increase in antioxidant protection. Although lifestyle factors have been considered as important modulators of DNA damage (Huang et al 2013;Fenech and Bonassi 2011;Wilson et al 2008), the main differences between groups should be considered as a consequence of the physical exercise training. Indeed, our results reinforce the association between higher daily physical activity and higher aerobic fitness with lower DNA strand breaks.…”

Section: Discussionmentioning

confidence: 99%

Effects of combined physical exercise training on DNA damage and repair capacity: role of oxidative stress changes

Soares

Silva

Oliveira

et al. 2015

AGE

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Regular physical exercise has been shown to be one of the most important lifestyle influences on improving functional performance, decreasing morbidity and all causes of mortality among older people. However, it is known that acute physical exercise may induce an increase in oxidative stress and oxidative damage in several structures, including DNA. Considering this, the purpose of this study was to identify the effects of 16 weeks of combined physical exercise in DNA damage and repair capacity in lymphocytes. In addition, we aimed to investigate the role of oxidative stress involved in those changes. Fifty-seven healthy men (40 to 74 years) were enrolled in this study. The sample was divided into two groups: the experimental group (EG), composed of 31 individuals, submitted to 16 weeks of combined physical exercise training; and the control group (CG), composed of 26 individuals, who did not undergo any specifically orientated physical activity. We observed an improvement of overall physical performance in the EG, after the physical exercise training. A significant decrease in DNA strand breaks and FPG-sensitive sites was found after the physical exercise training, with no significant changes in 8-oxoguanine DNA glycosylase enzyme activity. An increase was observed in antioxidant activity, and a decrease was found in lipid peroxidation levels after physical exercise training. These results suggest that physical exercise training induces protective effects against DNA damage in lymphocytes possibly related to the increase in antioxidant capacity.

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“…Este efeito sugere que partículas de bentonita na concentração empregada não gera citotoxicidade celular. No entanto, outros fatores precisarão ser avaliados para determinação da dosagem correta a ser empregada durante a realização de ensaios terapêuticos, como por exemplo, a avaliação do tempo de exposição (Maisanaba et al, 2013;Huang et al, 2013).…”

Section: Resultsunclassified

Partículas Argilominerais Não Influenciam a Viabilidade Das Células-Tronco Da Crista Neural

Nones¹,

Riella²,

Kuhnen³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -Partículas argilominerais apresentam inúmeras aplicações na indústria farmacêutica. Apesar de serem biotecnologicamente empregadas, seus efeitos sobre células-tronco da crista neural (CN) permanecem desconhecidos. Considerando estes aspectos, o objetivo deste trabalho foi caracterizar quimicamente partículas argilominerais (bentonita) extraídas da região Sul de Santa Catarina, assim como verificar sua influência sobre a viabilidade e sobrevivência das células-tronco da CN. Para tal, essas partículas argilominerais foram caracterizadas por difração de raios-X e ensaios físico-químicos. A viabilidade e morte das células foram determinadas por MTT e análise dos núcleos picnóticos, respectivamente. Nossos resultados demonstraram que partículas argilominerais (bentonita 0,6 mg/mL) não influenciam a viabilidade e a sobrevivência celular. Considerando as propriedades farmacológicas da bentonita, outros estudos deverão ser realizados com o intuito de avaliarmos seus efeitos em terapias envolvendo células-tronco.

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Genetic damage and lipid peroxidation in workers occupationally exposed to organic bentonite particles

Cited by 27 publications

References 28 publications

Short-Term Pulmonary Toxicity Assessment of Pre- and Post-incinerated Organomodified Nanoclay in Mice

Short-Term Pulmonary Toxicity Assessment of Pre- and Post-incinerated Organomodified Nanoclay in Mice

Effects of combined physical exercise training on DNA damage and repair capacity: role of oxidative stress changes

Partículas Argilominerais Não Influenciam a Viabilidade Das Células-Tronco Da Crista Neural

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