Genetic diversity among Texas bluegrass genotypes (Poa arachnifera Torr.) revealed by AFLP and RAPD markers

Renganayaki, K.; Read, J. C.; Fritz, A. K.

doi:10.1007/s001220000521

Cited by 46 publications

(32 citation statements)

References 29 publications

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“…A maioria dos acessos agrupouTabela 2. Relação dos iniciadores selecionados, com suas respectivas sequências e número de fragmentos polimórficos (NFP) gerados Estes acessos mais divergentes devem ter prioridades para futuros estudos, já que a melhoria das culturas através da utilização da diversidade genética presente no germoplasma é um fator chave para um programa de reprodução bem sucedido (Renganayaki et al, 2001). A variação de genética entre genótipos pode ser uma maneira prática de selecionar pais para a formação de uma progênie mais interessante tanto economicamente, como para a formação de indivíduos mais divergentes (Ahmad et al, 2010).…”

Section: M7 E M8 (Iv) O Terceiro Por M6 E M5 (Iii) E O úLtimo Por Munclassified

Divergência genética entre acessos de mangabeira (Hancornia speciosa Gomes)

Silva

Santos

Wickert³

et al. 2011

Agraria

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A mangaba (Hancornia speciosa Gomes) é uma espécie nativa do Brasil que apresenta importância econômica, social e cultural nas principais áreas onde ocorre. A preocupação com a devastação das áreas naturais da espécie resultou no estabelecimento de um Banco Ativo de Germoplasma, em Sergipe, o principal estado produtor. O objetivo do presente trabalho foi caracterizar a diversidade genética existente nesse BAG, utilizando marcadores RAPD. A utilização de 12 iniciadores de síntese permitiu a obtenção de 106 bandas, sendo 92 delas polimórficas (86,79%). Foram detectados diferentes coeficientes de similaridade, e a média entre eles foi de 0,40 (± 0,04), sendo que a amplitude genética variou entre 0,08 (± 0,04) e 0,60 (± 0,05). Os indivíduos M1 (Pará) e M14 (Bahia) foram os mais diferentes na população, e os pares de indivíduos M9 e M10 (Bahia) foram os mais semelhantes. Três pares de indivíduos foram identificados como mais divergentes (M2, M4 e M14) e indicados para futuros estudos.Palavras-chave: Banco de germoplasma, diversidade, RAPD. Genetic diversity between Hancornia speciosa Gomes varieties ABSTRACTHancornia speciosa Gomes is a native plant of Brazil of great economical, social and cultural importance in their areas of occurrence. The concern with the devastation of the specie natural areas resulted in the establishment of an Active Germplasm Bank in Sergipe, the main producing state. The objective of this work was to characterize the genetic diversity of this Germplasm Bank using RAPD analysis. The use of 12 RAPD primers allowed the production of 106 bands, in which 92 were polymorphic (86.79%). Different similarity coefficients were detected, with means of 0.40 (± 0.04) between them. The genetic amplitude varied between 0.08 (± 0.04) and 0.60 (± 0.05). The M1 (Pará) and M14 (Bahia) individuals were the most different ones within the population, and the individuals pairs M9 and M10 (Bahia) were the most similar ones. Three individuals pairs were identified as the most genetically divergent ones (M2, M4 and M14) and are indicated for future studies.

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Section: M7 E M8 (Iv) O Terceiro Por M6 E M5 (Iii) E O úLtimo Por Munclassified

Divergência genética entre acessos de mangabeira (Hancornia speciosa Gomes)

Silva

Santos

Wickert³

et al. 2011

Agraria

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“…Nonetheless, chickpea production is being constrained due to several biotic and abiotic stresses worldwide. Estimation and use of genetic diversity from the available genetic resources is a key factor for a successful breeding program (Renganayaki et al, 2001) aimed at improving crop performance under biotic and abiotic stresses. Variance of relatively highly heritable quantitative genetic markers provides estimates of genetic diversity, which may be utilized for selecting parents in the breeding programs.…”

Section: Introductionmentioning

confidence: 99%

Field Performance and Genetic Diversity of Chickpea Genotypes

Khan¹,

Ammar²,

Migdadi³

et al. 2016

IJAB

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Chickpea (Cicer arietinum L.) is an important legume crop widely grown across the globe. In this study, twenty six chickpea genotypes, of diverse origin, were evaluated for field performance and genetic diversity. Significant differences in morphological characters of tested chickpea genotypes were observed at vegetative and reproductive stages under field conditions. Amplified fragments length polymorphism (AFLP) and sequence-related amplified polymorphism (SRAP) markers, used in this study, showed considerable genetic diversity among the tested genotypes. With markers, 716 AFLP and 1164 polymorphic SRAP loci were analyzed using four AFLP and six SRAP primer combinations. The values for polymorphism information content for SRAP and AFLP were more than 0.6 and 0.9, respectively. In addition, many subclusters within genotypes were noted, which indicated high diversity level in the tested genotypes. Clustering of chickpea genotypes was largely based on genetic background and/or origin. Both morphological and molecular data helped to quantify the genetic diversity in tested chickpea genotypes and may be useful for the use in breeding program aimed at yield improvement.

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“…These molecular (DNA) markers allow the construction of a complete genome map and can be integrated with conventional linkage maps, which play an important role in plant breeding strategies (Simon and Muehlbauer, 1997). Improvement of crops through the utilization of available genetic diversity in the germplasm is a key factor for a successful breeding program (Renganayaki et al, 2001). Variance of relatively highly heritable quantitative genetic markers provides estimates of genetic diversity, and thus, genetic variation among diverse genotypes could be a practical way to select parents to be crossed.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis

Ahmad

Khan²,

Awan³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Genetic diversity analysis of chickpea germplasm can provide practical information for the selection of parental material and thus assist in planning breeding strategies. Chickpea seed is a good source of carbohydrates and proteins, constituting 80% of the total dry seed weight. Released cultivars and advanced lines of 30 chickpea genotypes were subjected to RAPD analysis for assessment of genetic diversity. We used 16 RAPD primers. Amplification of genomic DNA of the 30 genotypes yielded 62 fragments that could be scored. The number of amplification products produced per primer varied from two to four, with a mean of three bands. The total number of bands amplified by 16 anchored primers varied from 16 to 34. The primer GLK-15 produced the largest number (N = 4) of fragments, whereas primers GLK-19 and GLD-19 produced the smallest number (N = 1) of fragments. The single 1415 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (3): 1414-1420 (2010 Genetic diversity of chickpea germplasm in Pakistan band produced by the GTGTGCCCCA primer in the PB-2000 and 07005 genotypes may be attributed to temperature tolerance phenotypes.

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Genetic diversity among Texas bluegrass genotypes (Poa arachnifera Torr.) revealed by AFLP and RAPD markers

Cited by 46 publications

References 29 publications

Divergência genética entre acessos de mangabeira (Hancornia speciosa Gomes)

Divergência genética entre acessos de mangabeira (Hancornia speciosa Gomes)

Field Performance and Genetic Diversity of Chickpea Genotypes

Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis

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