Genetic Diversity Analysis between Different Varieties of Chickpea (Cicer arietinum L.) Using SSR Markers

Yadav, Summy; Shah, Vaidehi; Mod, Bhavya

doi:10.3126/ijasbt.v7i2.24634

Cited by 7 publications

(3 citation statements)

References 6 publications

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“…The bands are recorded as present or absent based on particular sizes. ISSR markers are highly polymorphic and characterized as dominant markers ( Yadav et al., 2019 ). However, to expand their usefulness, ISSRs can also be transformed into co-dominant markers such as Sequence Characterized Amplified Regions.…”

Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Amiteye

2021

Heliyon

131

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The concepts, methodologies and applications of some of the major molecular or DNA markers commonly used in plant science have been presented. The general principles of molecular marker techniques have been elucidated with detailed explanation of some notable basic concepts associated with marker applications: marker polymorphism, dominant or co-dominant mode of inheritance, agronomic trait-marker linkage, genetic mutations and variation. The molecular marker methods that have been extensively reviewed are RFLP, RAPD, SCAR, AFLP, SSR, CpSSR, ISSR, RAMP, SAMPL, SRAP, SSCP, CAPS, SNP, DArT, EST, and STS. In addition, the practicality of the retrotransposon-based marker methods, IRAP, REMAP, RBIP, and IPBS, have been discussed. Moreover, some salient characteristics of DNA markers have been compared and the various marker systems classified as PCR-or non-PCR-based, dominantly or co-dominantly inherited, locus specific or non-specific as well as at the levels of marker polymorphism and efficiency of marker reproducibility. Furthermore, the principles and methods of the following DNA markers have been highlighted: Penta-primer amplification refractory mutation system (PARMS), Conserved DNA-Derived Polymorphism (CDDP), P450-based analogue (PBA) markers, Tubulin-Based Polymorphism (TBP), Inter-SINE amplified polymorphism (ISAP), Sequence specific amplified polymorphism (S-SAP), Intron length polymorphisms (ILPs), Inter small RNA polymorphism (iSNAP), Direct amplification of length polymorphisms (DALP), Promoter anchored amplified polymorphism (PAAP), Target region amplification polymorphism (TRAP), Conserved region amplification polymorphism (CoRAP), Start Codon Targeted (SCoT) Polymorphism, and Directed Amplification of Minisatellite DNA (DAMD). Some molecular marker applications that have been recently employed to achieve various objectives in plant research have also been outlined. This review will serve as a useful reference resource for plant breeders and other scientists, as well as technicians and students who require basic know-how in the use of molecular or DNA marker technologies.

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Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Amiteye

2021

Heliyon

131

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“…Among them, Fusarium wilt caused by deuteromycetes fungal pathogen Fusarium oxysporum f. sp. ciceris (FOC) is one of the widely disbursed diseases of chickpea [4] and cause yield loss up to the level of 100% depending on varietal susceptibility and climatic conditions [5,6]. Developing wilt-resistant chickpea varieties necessitates labour and money intensive field level phenotyping of numerous germplasm and breeding lines against pathogen races.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Gene-based Markers against Fusarium Wilt Disease in Chickpea (Cicer Arietinum L.)

Rajput

Jain²,

Tiwari³

et al. 2023

CJAST

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Chickpea is an important legume crop owing to their nutritional worth. Due to the increasing population issue, it is needed to maintain the productivity of chickpeas to fulfill the requirements. At present, the main constraints in chickpea production are the Fusarium wilt disease. Forty genotypes of chickpea (Cicer arietinum L.) including RVG-203 as check variety were screened against Fusarium wilt resistance using six gene-based markers. Out of which five STMS markers showed polymorphism and amplified the alleles linked to resistance and susceptibility to Fusarium wilt disease in chickpea genotypes. The highest polymorphic information content (PIC) value was obtained with STMS Marker TR-29 and the least with STMS Marker TR-19. Based on molecular characterization, the genotypes produced similar allele as produced in the check variety RVG-203 and were identified as sources of resistance against Fusarium wilt. The results obtained in the present study open a window to use these genotypes as donor parents for the development of Fusarium wilt-resistant chickpea varieties through hybridization programs.

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“…cicerі -C. arietinum» шляхом комплементарного добору макро-і мікросимбіонтів [6][7][8][9]. Зважаючи на активну селекцію нових сортів нуту, яка проводиться в Україні та за кордоном [14][15][16], актуальним є вивчення їхньої здатності відкликатися на інокуляцію ефективними штамами ризобій.…”

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Screening of Modern Chickpea Varieties by Response to Bacterization

Lohosha¹,

Yu.²,

Usmanova³

et al. 2021

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Objective. To screen modern varieties of chickpeas of Ukrainian selection by the response to bacterization, to identify varieties characterized by high increases in symbiotic activity and yield. Methods. Biotesting, gas-chromatographic, field experiment, statistical. Results. The response to bacterization of chickpea seeds of different varieties was studied in a field experiment in the southern region of Ukraine (Region of Odesa). As a result of inoculation of chickpea seeds with a bacterial suspension of an effective strain of Mesorhizobium ciceri ND-64, a significant increase in nodulation activity was registered. The highest increase in the number of nodules (51.6 % and 53 %) was reported for the varieties Odysei and Triumf. Upon seed inoculation with the studied strain, a significant increase in the weight of nodules of the Triumf variety (2.3 times) was shown. In the control variants, the nitrogenase activity in the nodules of Skarb variety plants was the highest (425 nmol C2H4/plant per hour), and in the Budzhak variety it was the lowest (159 nmol C2H4/plant per hour). Inoculation of seeds with bacterial suspension of M. ciceri ND-64 contributed to a significant increase in the studied parameter in the varieties Budzhak, Pamiat, Odysei and Triumf. The latter was characterized by the most significant response to inoculation with an effective strain in terms of nodulation and nitrogenase activity. Symbiotic parameters showed the weakest ability to respond to inoculation in terms of symbiotic parameters in Skarb variety. Pamiat variety was characterised by the maximum number of seeds per plant (18.5 units/plant). However, as a result of inoculation of chickpea seeds with M. ciceri ND-64, the number of seeds increased the most in the variety Skarb and amounted to 28.7 units/plant. In terms of seed weight, the Triumph variety was superior (9.5 g/plant). The yield of all studied varieties of chickpeas after bacterization of seeds with M. ciceri ND-64 significantly exceeded the control variants (without inoculation) by 21–56 %. The highest increase in control was observed for the varieties Triumph (0.49 t/ha) and Skarb (0.56 t/ha). The ability of nodule bacteria M. ciceri ND-64 to intensive synthesis of phytohormones and the formation of highly effective symbiosis with chickpea plants of different varieties provided a complementary interaction of rhizobia with plants and a significant increase in crop yield. Conclusion: Due to the interaction with aboriginal nodule bacteria, the highest parameters of nodulation and nitrogenase activity were typical for the varieties Triumf and Skarb, yields for Triumf and Budzhak. Screening of varieties by the response to inoculation with an effective strain of M. ciceri ND-64 indicates an increase in symbiotic activity in varieties Triumf, Budzhak (large-seeded), Pamiat (medium-seeded). The maximum increase in yield as a result of inoculation was found in chickpea varieties Skarb and Triumf. M. ciceri ND-64 are capable of active synthesis of substances of phytohormonal nature, which contributes to the effective interaction between rhizobia and chickpea plants.

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Genetic Diversity Analysis between Different Varieties of Chickpea (Cicer arietinum L.) Using SSR Markers

Cited by 7 publications

References 6 publications

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Evaluation of Gene-based Markers against Fusarium Wilt Disease in Chickpea (Cicer Arietinum L.)

Screening of Modern Chickpea Varieties by Response to Bacterization

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