Genetic diversity analysis in date palm (<i>Phoenix dactylifera</i>L.): a comparative assessment using ISSR and RAPD marker assays

Mitra, Charu; Kharb, Pushpa; Uppal, S.; Jain, Sunita

doi:10.1080/14620316.2011.11512780

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…These results indicated the efficiency of UBC814, UBC826 and UBC868 primers in identifying the genetic diversity and the relationship among the varieties based on the ISSR-PCR polymorphic banding profiles. Several studies applied the ISSR primers to determine the genetic polymorphism among date palm varieties (Mitra et al, 2011;Zehdi et al, 2002) or other plant species such as tomato (Al-Khayri et al, 2023) and Kalanchoe (Al-Khayri et al, 2022). Similar results were found by Hamza et al (2012) where a set of different ISSR primers were used to amplify DNA from 26 different date palm varieties.…”

Section: Phylogenetic Analysesmentioning

confidence: 83%

“…Moreover, some studies investigated the relationship between phenotypic and molecular markers. Mitra et al (2011) highlighted the ability to use ISSR markers to distinguish between male and female date palm cultivars. This study confirmed that molecular markers are a powerful tool for exploring the genetic diversity of plants (Agarwal et al, 2008).…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

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Genetic diversity assessment and in vitro propagation of some date palm (Phoenix dactylifera L.) varieties

ABDELGHAFFAR,

ALSHEGAIHI,

ALKHATEEB

et al. 2023

Not Bot Horti Agrobo

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The evaluation of genetic diversity is crucial for breeders to develop strategies and improve the resilience, quality, and adaptability of the date palm. In this study, the genetic diversity of three date palm varieties was performed using ISSR-PCR molecular markers to determine its relationship with in vitro propagation response of these varieties. The molecular profiling was performed using ISSR-PCR. A total of 49 loci were produced by the PCR reactions, 38 of which were polymorphic while 11 were monomorphic. The level of polymorphism revealed by ISSR-PCR varied from 33.33% to 100%. The three date palm varieties were grouped into two clusters based on the results of cluster analyses that used morphological data and molecular profiles. Cluster I comprised the ‘Barhy’ variety and Cluster II included ‘Magdoul’ and ‘Amri’ varieties. The clustering analyses revealed the independence of the ‘Barhy’ variety in its characteristics from the other varieties based on either morphological or molecular data. The results of in vitro propagation showed that the ‘Amri’ variety exhibited the highest callus induction frequency (86.28%), callus weight (2.33 g), number of somatic embryos (9.32), number of shoots (14.62), number of roots (4.11), root length (4.63 cm), shoot length (13.61 cm) followed by ‘Magdoul’ and ‘Barhy’ varieties. The ‘Amri’ variety had the shortest callus induction period, at 23.26 days while the ‘Barhy’ variety exhibited the longest period of callus induction (28.55). It was deduced from the study that the ISSR marker reproduced trustworthy patterns of bands to determine the genetic diversity among different date palm varieties that are considered the cornerstone for the genetic improvement of date palms. The understanding of the relationship between genetic diversity and in vitro propagation response of date palm is essential for ensuring the long-term sustainability of its crop. This will facilitate better conservation and development of new date palm varieties that fulfil the needs of farmers and consumers.

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Section: Phylogenetic Analysesmentioning

confidence: 83%

Section: Phylogenetic Analysesmentioning

confidence: 99%

Genetic diversity assessment and in vitro propagation of some date palm (Phoenix dactylifera L.) varieties

ABDELGHAFFAR,

ALSHEGAIHI,

ALKHATEEB

et al. 2023

Not Bot Horti Agrobo

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“…In the present investigation, three introduced varieties and five local landraces of Phoenix dactylifera L. were analyzed for polymorphism based on ISSR analysis. This technique has been already exploited to detect polymorphic differences between different date palm varieties [34][35][36][46][47][48][49][50][51][52]. For the present investigation sample was taken from five local landraces and three introduced varieties to characterize and assess the genetic variation by using 21 ISSR primers.…”

Section: Genetic Distance Within Local Landracesmentioning

confidence: 99%

“…Another study using 8 RAPD observed 36% of polymorphism and 32.09 % of polymorphism observed using 8 ISSR primers [54] and 28 AFLP primers in combinations to examine the phylogenetic relationships among five Upper Egypt date palm cultivars and observed 41.59% polymorphism across cultivars [18]. However the polymorphism percentage obtained among all samples of collected date palm was less when compared to a date palm diversity study [46] respectively and it was less than those found by two groups, [50,55] and who amplified the bands sizes ranged from 200 to 2000-bp and 100 to 1400 bp respectively. The product size obtained in our study is somewhat similar with a study [56], obtained 50-700 bp.…”

Section: Genetic Distance Within Local Landracesmentioning

confidence: 99%

Molecular characterization of some landraces and varieties of Date palm (Phoenix dactylifera L.) from Afar region of Ethiopia using ISSR markers

Indracanti

Mekonnen

Tsegaw

2019

Preprint

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Back ground Date palm (Phoenix dactylifera L.) is one of the oldest fruit trees in hot arid region of the world including North Africa. In some areas of Afar region of Ethiopia, date palm grow as landraces, are in danger due to introduction of improved cultivars. Present study was carried out to fill knowledge gap about molecular diversity of this crop in Afar region. Molecular studies of 5 landraces and 3 introduced cultivars of date palm from Afar region of Ethiopia were tested using 21 randomly selected ISSR primers for amplification and polymorphism detection using genomic DNA. ISSR markers across 8 date palm varieties were scored for their presence (1) or absence (0). Shannon's Information index (I) and polymorphic information content (PIC) were analyzed by popGENE 32 and online PIC calculator respectively. Results 17 out of 21 ISSR markers used for this study produced a total of 557 scorable DNA fragments with average of 33.52 per marker and 61.68, 43.93 and 68.22% polymorphism were obtained within local landraces, introduced varieties and among all samples respectively. The genetic distance among all samples ranged from 0.1402 to 0.5953; and the dendrogram separated date palm varieties into seven clusters. ISSR markers used for this study have high discrimination power and the average values of Shannon's information index and PIC were 0.318 and 0.76 respectively. Conclusion Genetic diversity was observed among all date palms studied in this investigation. To have better understanding on genetic diversity of date palm in the Afar region, further research should be done using SNP markers and landraces should be registered.

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Genetic Diversity and Selection of Suitable Molecular Markers for Characterization of Indigenous Zizyphus Germplasm

2019

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Genetic diversity analysis in date palm (Phoenix dactyliferaL.): a comparative assessment using ISSR and RAPD marker assays

Cited by 7 publications

References 7 publications

Genetic diversity assessment and in vitro propagation of some date palm (Phoenix dactylifera L.) varieties

Genetic diversity assessment and in vitro propagation of some date palm (Phoenix dactylifera L.) varieties

Molecular characterization of some landraces and varieties of Date palm (Phoenix dactylifera L.) from Afar region of Ethiopia using ISSR markers

Genetic Diversity and Selection of Suitable Molecular Markers for Characterization of Indigenous Zizyphus Germplasm

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