Genetic diversity analysis in different genotypes of black gram [Vigna mungo (L.) Hepper] using RAPD marker

Abstract

Amongst the various DNA fingerprinting methodologies, randomly amplified polymorphic DNA (RAPD) was used to estimate genetic diversity and relationship amongst 22 black gram genotypes. A total of 25 randomly selected decamers were screened, out of which only 16 got amplified. A total of 133 amplified bands were obtained, out of which 120 were polymorphic. The average percentage of polymorphism was 90.23. The total number of amplified bands varied between 3 (primer OPK-03) and 15 (primer OPC-08) with an average of 9 bands per primer. The overall size of PCR amplified products ranged between 200 bp to 2600 bp. The average PIC was 0.30 ranging from 0.17 to 0.43. Five unique bands (ranging from 200-1200 bp) were detected in four genotypes using 5 RAPD primers. Jaccard's similarity coefficient values for RAPD primers ranged from 0.58-0.85 with an average of 0.71. Based on dendrogram generated through UPGMA method and PCA, most of the genotypes got divided into three main clusters. Genotypes U-17 and STY-2289 were lying close and thus showed minimum genetic distance while genotypes UH-177 and IPU99-233 had minimum similarity value of 0.42, thus showing maximum divergence. Thus, these results could be used to assess other black gram accessions in the Vigna germplasm pool that can provide useful information towards molecular classification and the genetic marker assisted breeding for crop improvement.

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

“…Maximum PIC value (0.46) was recorded for UBC 836 among primers. The results of PIC values are conformity with genetic diversity of 22 black gram genotypes by using 15 RAPD primers (Vyas et al, 2016). Similarly the highest PIC value (0.55) Bishoy et al, (2014) reported UBC 887 in genetic diversity study of 17 accessions of Clitoria ternatea using ISSR markers.…”

RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java

“…Four loci of 400, 600, 1,100 and 2,200 bp in length are observed. Extremely distinct result with OPP-08 showing a 100% polymorphism in black gram (Vigna mungo) was reported (Vyas et al, 2016). In addition, OPP-08 resulted in 91.7% polymorphism in fenugreek (Trigonella foenum-graecum) (Tomar et al, 2014).…”

RAPD assisted selection of black gram (Vigna mungo L. Hepper) towards the development of multiple disease resistant germplasm

“…These primers were synthesized by Sigma-Aldrich and the sequences were used in previous studies on disease resistance in black gram (Basak et al 2004; Vyas et al 2016). Of the 40, only 33 RAPD primers produced clear and reproducible banding patterns.…”

“…However, high genetic consistency and the absence of suitable ideotypes reinforce with deficient harvest and disease susceptibility has caused major constraints in achieving a higher yield (Vyas et al 2016). Among various biotic factors affecting black gram yield, the main diseases which devastate the crops are mung bean yellow mosaic virus (MYMV), urdbean leaf crinkle virus (UCLV), powdery mildew (PM) and wilt ( Fusarium oxysporum ).…”