Genetic Diversity Analysis in the Genus Aloe vera (L.) Using RAPD 
 and ISSR Markers

Bhaludra, Chandra Sekhar Singh; Hari, Yosuke; Cyprian, Farhan S.; Bethapudi, Rama Rao; Basha, S.D.; Anupalli, Roja Rani

doi:10.3923/ijp.2014.479.486

Cited by 5 publications

(3 citation statements)

References 8 publications

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“…The percentage of polymorphic bands observed within the fifteen populations ranged from 29% to 42% in RAPD and 39% to 63% in ISSR markers. Similar findings have been reported in various studies; [29] obtained a higher polymorphism using ISSR markers (80.9 %) than RAPD (71.8 %) while studying genetic diversity of Aloe vera. However, low levels of polymorphism using both ISSR and RAPD markers were reported in Bruguirra gymnorrhiza and Heritiera fomes [30].…”

Section: Population Genetic Parameters: Percentage Of Polymorphic Bsupporting

confidence: 91%

“…In India, [28,41] used RAPD and AFLP markers to assess genetic diversity among Aloe accessions from different geographical regions. Similar results have been reported by [10] and [29] on genetic variability in Aloe vera where both RAPD and ISSR primers have been used. Moreover, in this study, there was genetic diversity in each population as revealed by the Nei's genetic diversity indices (H) which varied between the ISSR and RAPD markers.…”

Section: Population Genetic Parameters: Percentage Of Polymorphic Bsupporting

confidence: 87%

“…In the past, researchers have reported genetic diversity studies in Aloe species using RAPD and ISSR markers among other different marker systems. This is because of their simplicity, rapidness, affordability and can be applied without any prior knowledge regarding the plant genome [29,38]. Both RAPD and AFLP (Amplified Fragment Length Polymorphism) markers have also been used to study the genetic diversity among different Aloe species [39,40].…”

Section: Population Genetic Parameters: Percentage Of Polymorphic Bmentioning

confidence: 99%

See 2 more Smart Citations

Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

Adienge¹,

Muturi²,

Nadir³

et al. 2019

J Plant Sci Mol Breed

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Aloe species are common in arid and semi-arid lands (ASALs) of Kenya and are of economic importance especially for bitter gum production. However, the species is under ecological threat due to unsustainable harvesting from the wild. The objective of this study was to evaluate genetic diversity and population structure of three commercially exploited indigenous Aloe species; A. secundiflora, A. turkanensis and A. scabrifolia.The study was carried out in fifteen populations of Aloe species in their wild habitat where their geographic distribution was surveyed and populations delineated. Within each population, 30 trees were sampled at a distance of 100 m apart where leaf tissues were collected for DNA isolation and analysis using modified Cetyl trimethyl ammonium bromide (CTAB) method. Randomly amplified polymorphic DNA (RAPDs) and Inter-simple sequence repeats (ISSR) markers were used to determine the genetic diversity and population structure. Ten RAPD and seven ISSR discriminative primers with the highest value of genetic diversity were selected to genotype three indigenous Aloe species. The genetic similarity was performed using Popgene 1.3.1; hierarchical UPGMA cluster analysis and principal coordinate analysis were performed using Genalex 6.5. To map genetic structure and gene pools, both Structure and Structure Harvester statistical packages were used. From the results, A. secundiflora species was the most distributed in the surveyed ASALs. There was high genetic variation among and within the populations. The combined use of ISSR and RAPD revealed high variations among the populations as compared to when either of them used singly. Genetic variation was highest within secundiflora species and least within turkanensis species. Maralal secundiflora population had the highest genetic variation. The study exhibited a population genetic structure with three major clusters which corresponded to the three Aloe species with minimal reproductive crossing among them. It is therefore, recommended to undertake biophysical studies to determine the advantages of A.secundiflora that has over the other species. In addition, Maralal sec population should be conserved ex-situ because of its high genetic diversity while both ISSR and RAPD markers should be prioritized in Aloe genetic studies as they reveal high variation.

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Section: Population Genetic Parameters: Percentage Of Polymorphic Bsupporting

confidence: 91%

Section: Population Genetic Parameters: Percentage Of Polymorphic Bsupporting

confidence: 87%

Section: Population Genetic Parameters: Percentage Of Polymorphic Bmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

Adienge¹,

Muturi²,

Nadir³

et al. 2019

J Plant Sci Mol Breed

View full text Add to dashboard Cite

show abstract

Elucidation of genetic diversity and population structure of sixty genotypes of Aloe vera using AFLP markers

Kumar

Salar

Naik

et al. 2022

South African Journal of Botany

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Molecular characterization of some Indian Aloe vera populations through RAPD and ITS markers

Das

Nandagopal

Jha³

2016

Plant Biosystems - An International Journal Dealing with all As

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Genetic Diversity Analysis in the Genus Aloe vera (L.) Using RAPD and ISSR Markers

Cited by 5 publications

References 8 publications

Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

Elucidation of genetic diversity and population structure of sixty genotypes of Aloe vera using AFLP markers

Molecular characterization of some Indian Aloe vera populations through RAPD and ITS markers

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