Genetic diversity analysis of date palm (Phoenix dactylifera L.) in the Kutch<br>region of India using RAPD and ISSR markers

Abstract

First line in abstract should be Date palm is an important fruit crop of the arid and semiarid region. In the present investigation two types of DNA based marker RAPD and ISSR were used to characterize 8 date palm genotypes grown in the Kutch region. Amplification of genomic DNA of 8 genotypes using 13 RAPD analyses yielded 88 fragments, of which 35 were polymorphic, with an average of 2.69 polymorphic fragments per primer. Two ISSR primers produced 13 bands of which 3 were polymorphic. RAPD markers were more efficient than ISSR assay with regard to polymorphic detection; RAPD markers detected 39.77% as compared to 23.07% of ISSR markers. Cluster analysis by UPGMA showed that the dendrogram obtained by RAPD and RAPD + ISSR were similar. Cluster A consisted of Early maturing, Ghanshyam and Late maturing female genotypes with 0.81 to 0.88 Jaccard's similarity range. Cluster B consisted of Seasonal female, Male-1, Male-2, Male-3 and Male-4 genotypes with 0.82 to 0.91 similarity range. Genotypes Male-1 and Male-2 were most closely related with the highest value in similarity for Jaccard's coefficient (0.91). Principal coordinate analysis differentiated one group of genotype Male-1 and Male-4 while other genotypes were randomly distributed.

Increased relatedness among the neighboring plants from seedling to adult stages in carnaúba wax palm

“…Srivashtav et al (2013) used two ISSR primers to assess the genetic diversity of the species Phoenix dactylifera L. (Arecaceae) from a region in India. Total thirteen loci were found, among which 10 were polymorphic and three were monomorphic.…”

Genetic Diversity Analysis of Soursop (Annona muricata L.) in West Java Region of Indonesia Using RAPD Markers

“…The size of the amplification product ranged from 150 to 1000bp. The highest number of bands (15) were obtained with primer OPE-4, while the lowest number of bands (9) were obtained with primer OPH-13. The percentage of polymorphism ranged from 40 to 81.82%.…”

Assessment Of Rapd Polymorphism In Maize (Zea Mays L.) Genotypes

“…RAPD technique requires only small amounts of DNA sample without involving radioactive labels and are simpler as well as faster. Random amplified polymorphic DNA has proven to be quite efficient in detecting genetic variations and used for diversity assessment and for identifying germplasm in a number of plant species (Gajeraa et al, 2010;Srivashtav et al, 2013;Žiarovská et al, 2013;Omalsaad et al, 2014;Vivodík et al, 2014).…”