Genetic diversity and antibiotic resistance of <i>Shigella</i> spp. isolates from food products

Pakbin, Babak; Amani, Zahra; Allahyari, Samaneh; Mousavi, Shaghayegh; Mahmoudi, Razzagh; Brück, Wolfram M.; Peymani, Amir

doi:10.1002/fsn3.2603

Cited by 23 publications

(28 citation statements)

References 45 publications

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“…This study found that resistance against trimethoprim-sulfamethoxazole, tetracycline, lineage III strains due to the acquisition of point mutations within the gyrA and parC genes (Hawkey, et al 2021, Phiri, et al 2021, Shad and Shad 2021. It is extremely important to monitor different S. sonnei lineages/sublineages and the antibiotic resistance profile of these strains as the new emerging threat to public health and global concern of food safety (Hawkey, et al 2021, Pakbin, et al 2021a, Pakbin, et al 2021c.…”

Section: Discussionmentioning

confidence: 94%

“…However, resistance against different antibiotics is becoming more common among the S. sonnei isolated from food and clinical samples. It is widely reported that some specific genotypes of S. sonnei are associated with high rates of multi-drug resistance and more frequently contribute to foodborne outbreaks (Pakbin, et al 2020a, Pakbin, et al 2021a, Ranjbar and Farahani 2019.…”

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confidence: 99%

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Phylogenetic analysis and antibiotic resistance of Shigella sonnei isolates

Pakbin

Didban

Alizadeh

2022

FEMS Microbiology Letters

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Shigellosis is one of the most important gastric infections caused by different species of Shigella, and has been regarded as a serious threat to public health. Lineage/sublineage profile of Shigella sonnei is strongly associated with the antibiotic resistance and population structure of this pathogen. In this study, we determined the phylogeny and antibiotic resistance profiles of S. sonnei strains, isolated from 1246 stool and 580 food samples, using multiplex PCR–HRMA genotyping and Kirby–Bauer disk diffusion methods, respectively. A total of 64 S. sonnei strains were isolated (13 food and 51 clinical isolates). Multiplex PCR–HMR assay was able to differentiate the lineages II and III, and sublineages IIIb and IIIc strains successfully considering the definite melting curves and temperatures. Lineage I and sublineage IIIa strain were not isolated in this study. We also demonstrated that most of the S. sonnei strains isolated from both food and clinical samples clustered within the lineage III and sublineage IIIc. Resistance against trimethoprim-sulfamethoxazole, tetracycline, chloramphenicol, and streptomycin antibiotics were the most prevalent phenotypes among the S. sonnei lineage III and sublineage IIIc strains.

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Section: Discussionmentioning

confidence: 94%

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confidence: 99%

Phylogenetic analysis and antibiotic resistance of Shigella sonnei isolates

Pakbin

Didban

Alizadeh

2022

FEMS Microbiology Letters

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“…Outbreaks of Sh. flexneri have been linked to unpasteurized vegetable juices and meat in recent years [ 35 , 36 ]. Eugenol had a bactericidal effect on Sh.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Effect of Eugenol on Shigella flexneri and Its Mechanism

Bai

et al. 2022

Foods

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Shigella flexneri (Sh. flexneri), which can be found in food and the environment, is a widespread food-borne pathogen that causes human diarrhea termed “shigellosis”. In this study, eugenol, a natural active substance, was investigated for its antibacterial activity against Sh. flexneri. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of eugenol against Sh. flexneri ATCC 12022 was 0.5 and 0.8 mg/mL. The growth curves and inhibitory effect in LB broth, PBS, vegetable juice, and minced pork showed that eugenol had a good activity against Sh. flexneri. Research findings indicated the superoxide dismutase activity of Sh. flexneri was inhibited after eugenol treatment, resulting in concentrations of intracellular reactive oxygen species and an increase in malondialdehyde. The flow cytometry analysis and field emission scanning electron microscopy results revealed obvious damage to cell membrane integrity and changes in the morphology of Sh. flexneri. In addition, the intracellular ATP concentration leaked from 0.5 μM to below 0.05 μM and the membrane potential showed a concentration-dependent depolarization after eugenol treatment. In summary, eugenol exerted strong antibacterial activity and has the potential to control Sh. flexneri in the food industry.

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“…Shigella is a bacterium related with E. coli, which is classified into four serogroups according to their species: Shigella dysenteriae 1 (serogroup A), S. flexneri (serogroup B), Shigella boydii (serogroup C), and Shigella sonnei (serogroup D) (Jo et al, 2019;Pakbin et al, 2021). The serogroups from S. sonnei and S. flexneri are the most frequently isolated in vegetable salad samples (Pakbin et al, 2021). In animal cells, it has been found that the effector protein IpaB, secreted by the T3SS of S. flexneri, facilitates their adherence (Rasmussen et al, 2005) and the L-asparaginase (AnsB) protein is also necessary for the strain for full virulence (George et al, 2014).…”

Section: Other Microorganisms That Interact With Plantsmentioning

confidence: 99%

Adhesion to and survival of foodborne pathogens in produce and strategies for their biocontrol

Barba‐León,

Arista‐Regalado,

Mancilla‐Becerra

et al. 2024

Journal of Food Safety

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Foodborne pathogens can cause gastrointestinal infections in consumers and in some cases can even lead to outbreaks. In the last decade, it has been observed that some zoonotic pathogenic bacteria can use plants as secondary hosts. Contamination with foodborne bacteria becomes relevant in foods that are regularly eaten raw, such as lettuce, cilantro, fenugreek, rocket leaves, basil, and so forth, and some fruits such as tomatoes, melons, and green peppers; because the elimination of these pathogenic bacteria is difficult to achieve with conventional sanitization processes. Contamination of produce can occur throughout the entire production chain. In farmlands, pathogenic bacteria can contaminate the seed, mainly when contaminated water is used for irrigation. Later, bacteria can reach other plant tissues such as the stems, leaves, and fruits. Another form of contamination is when the produce is in contact with feces from domestic, production, or wild animals. Additionally, poor handling practices during harvest, packaging, distribution, and sale can contaminate produce. Studies have shown that foodborne pathogens can adhere to produce, sometimes forming a biofilm, and can also be internalized into the plant or fruit, which protects them from sanitation processes. For this reason, in this text we address three biocontrol strategies such as bacteria, lytic bacteriophages, and some fungi, as an alternative approach for the control of both foodborne and plant pathogens. Additionally, the use of these biological agents can represent an advantage for the development of the plant, making them a good strategy to favor yield.

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Genetic diversity and antibiotic resistance of Shigella spp. isolates from food products

Cited by 23 publications

References 45 publications

Phylogenetic analysis and antibiotic resistance of Shigella sonnei isolates

Phylogenetic analysis and antibiotic resistance of Shigella sonnei isolates

Antibacterial Effect of Eugenol on Shigella flexneri and Its Mechanism

Adhesion to and survival of foodborne pathogens in produce and strategies for their biocontrol

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