Genetic diversity and evolutionary history of four closely related <i>Aquilegia</i> species revealed by 10 nuclear gene fragments

Huang, Lei; Geng, Fang‐Dong; Fan, Jingjing; Xue, Cheng; Zhang, Xiaoyan; Kang, Ju‐Qing; Zhang, Jianqiang; Ren, Yi

doi:10.1111/jse.12298

Cited by 12 publications

(17 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…kansuensis should be considered as a separate species rather than a variety. The phylogenetic tree shows that A. ecalcarata sequences were present on two different branches, providing further evidence to the previous report that A. ecalcarata is not monophyletic with a single origin and may have a complicated evolutionary history (Huang et al, 2018). In the future, to infer the phylogenetic relationships of rapidly evolving species within Aquilegia , we should collect more varieties and a greater number of species to construct the phylogeny.…”

Section: Discussionsupporting

confidence: 85%

Comparative chloroplast genomes and phylogenetic analysis of Aquilegia

et al. 2021

View full text Add to dashboard Cite

Aquilegia is an ideal taxon for studying the evolution of adaptive radiation. Current phylogenies of Aquilegia based on different molecular markers are inconsistent, and therefore a clear and accurate phylogeny remains uncertain. Analyzing the chloroplast genome, with its simple structure and low recombination rate, may help solve this problem. METHODS: Next-generation sequencing data were generated or downloaded for Aquilegia species, enabling their chloroplast genomes to be assembled. The assemblies were used to estimate the genome characteristics and infer the phylogeny of Aquilegia. RESULTS: In this study, chloroplast genome sequences were assembled for Aquilegia species distributed across Asia, North America, and Europe. Three of the genes analyzed (petG, rpl36, and atpB) were shown to be under positive selection and may be related to adaptation. The phylogenetic tree of Aquilegia showed that its member species formed two clades with high support, North American and European species, with the Asian species being paraphyletic; A. parviflora and A. amurensis clustered with the North American species, while the remaining Asian species were found in the European clade. In addition, A. oxysepala var. kansuensis should be considered as a separate species rather than a variety. DISCUSSION: The complete chloroplast genomes of these Aquilegia species provide new insights into the reconstruction of the phylogeny of related species and contribute to the further study of this genus.

show abstract

Section: Discussionsupporting

confidence: 85%

Comparative chloroplast genomes and phylogenetic analysis of Aquilegia

et al. 2021

View full text Add to dashboard Cite

show abstract

“…On the other hand, spur loss could have happened in a stepwise fashion, with mutations occurring first in these other loci affecting smaller aspects of spur morphology before mutations eventually occurred at POP. Studies focusing on population genetics in A. ecalcarata and its closest relatives may help clarify these scenarios (35). In either case, it is interesting to note that the major component of spur loss in A. ecalcarata appears to be caused by changes in POP expression, rather than function, perhaps because POP has pleiotropic effects on other traits such as leaf development.…”

Section: Discussionmentioning

confidence: 99%

POPOVICH, encoding a C2H2 zinc-finger transcription factor, plays a central role in the development of a key innovation, floral nectar spurs, inAquilegia

Ballerini

Min

Edwards

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The evolution of novel features, such as eyes or wings, that allow organisms to exploit their environment in new ways can lead to increased diversification rates. Therefore, understanding the genetic and developmental mechanisms involved in the origin of these key innovations has long been of interest to evolutionary biologists. In flowering plants, floral nectar spurs are a prime example of a key innovation, with the independent evolution of spurs associated with increased diversification rates in multiple angiosperm lineages due to their ability to promote reproductive isolation via pollinator specialization. As none of the traditional plant model taxa have nectar spurs, little is known about the genetic and developmental basis of this trait. Nectar spurs are a defining feature of the columbine genus Aquilegia (Ranunculaceae), a lineage that has experienced a relatively recent and rapid radiation. We use a combination of genetic mapping, gene expression analyses, and functional assays to identify a gene crucial for nectar spur development, POPOVICH (POP), which encodes a C2H2 zinc-finger transcription factor. POP plays a central role in regulating cell proliferation in the Aquilegia petal during the early phase (phase I) of spur development and also appears to be necessary for the subsequent development of nectaries. The identification of POP opens up numerous avenues for continued scientific exploration, including further elucidating of the genetic pathway of which it is a part, determining its role in the initial evolution of the Aquilegia nectar spur, and examining its potential role in the subsequent evolution of diverse spur morphologies across the genus.

show abstract

“…The results from the systematic molecular (Fior et al, ) and genetic diversity studies (Huang et al, ) showed that A . yabeana might be the ancestral species within the monophyletic clade, which consists of A .…”

Section: Discussionmentioning

confidence: 99%

“…kansuensis and one population similar to A . rockii (Huang et al, ), indicating the genetic differences within A. ecalcarata and the possible multiple origination of the spurless status. The present morphological study showed that, morphologically, all populations of A .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological variation pattern ofAquilegia ecalcarataand its relatives

Xue

Geng

Zhang

et al. 2019

J of Sytematics Evolution

Self Cite

View full text Add to dashboard Cite

Aquilegia ecalcarata Maxim. is the only spurless species within the genus Aquilegia and comprises a monophyletic clade with A. yabeana Kitag., A. kansuensis Brühl, and A. rockii Munz. Our previous study on the genetic diversity of those four species revealed that the populations of A. ecalcarata can be divided into two groups, indicating possible genetic difference within A. ecalcarata. However, it is not clear whether the genetic difference is related to the morphological variation among species and groups of A. ecalcarata populations. To answer that question, the morphological variation patterns based on 22 floral and 19 vegetative traits from 42 populations, covering the entire distribution of A. ecalcarata and its relatives, were analyzed in the present study. The result showed that: (i) the differences among the four species were reflected in the floral rather than the vegetative traits; (ii) populations of A. yabeana and A. rockii fell into one cluster each, and each of the six clusters occupied its own distribution range; (iii) one of two A. ecalcarata clusters fell into a subgroup and shared common floral traits with A. rockii; (iv) the individuals of A. ecalcarata form. ecalcarata and form. semicalcarata were often mixed in the same population; and (v) the populations of A. kansuensis were split into two clusters, which differed obviously in floral traits. These results will provide an important morphological basis for the redefinition of species and lay a foundation for the further exploration of the "spurless" A. ecalcarata.

show abstract

Genetic diversity and evolutionary history of four closely related Aquilegia species revealed by 10 nuclear gene fragments

Cited by 12 publications

References 51 publications

Comparative chloroplast genomes and phylogenetic analysis of Aquilegia

Comparative chloroplast genomes and phylogenetic analysis of Aquilegia

POPOVICH, encoding a C2H2 zinc-finger transcription factor, plays a central role in the development of a key innovation, floral nectar spurs, inAquilegia

Morphological variation pattern ofAquilegia ecalcarataand its relatives

Contact Info

Product

Resources

About