Genetic diversity and evolutionary patterns of <i>Taraxacum kok‐saghyz</i> Rodin

Zhang, Yan; Ren, Hailong; Zhang, Xuechao; Wang, Li; Gao, Qiang; Abudurezike, Abudukeyoumu; Yan, Qingqing; Lu, Zengkui; Wang, Yonggang; Nie, Qiuhai; Xu, Lin; Zhang, Zhibin

doi:10.1002/ece3.7622

Cited by 8 publications

(5 citation statements)

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“…Genetic mapping is essential for QTL mapping, map-based cloning, and MAS [ 29 ] and has been widely used in many major crops [ 17 , 27 , 28 , 30 ]. In the past decade, although a great quantity of TKS molecular markers (SSRs and SNPs) have been developed, they have mostly been used in research on the genetic diversity and population structure of TKS [ 7 , 20 , 21 , 23 , 24 ]. In 2016, the first TKS genetic map consisting of eight LGs was constructed, which included 1448 AFLPs, 6 COSs, 1 SSR and 63 EST-SSRs [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Among them, 50 SNPs were obtained from 39 transcripts related to rubber biosynthesis, and 117 SNPs were found in 36 differentially expressed gene sequences. In 2021, a total of 524,812 SNPs were obtained by genotyping-by-sequencing (GBS) and were used for population structure, genetic diversity, and evolutionary analysis of 58 wild TKS plants collected from four different regions [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Construction of the first high-density SNP genetic map and identification of QTLs for the natural rubber content in Taraxacum kok-saghyz Rodin

et al. 2023

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Background Taraxacum kok-saghyz Rodin (TKS) is a promising commercial alternative natural rubber (NR) yielding plant. Cultivating TKS with a high NR content is an important breeding target, and developing molecular markers related to NR content can effectively accelerate the breeding process of TKS. Results To construct a high-density SNP genetic map and uncover genomic regions related to the NR content in TKS, an F1 mapping population of TKS was constructed by crossing two parents (l66 and X51) with significant differences in NR contents. The NR content of the F1 plants ranged from 0.30 to 15.14% and was distributed normally with a coefficient of variation of 47.61%, indicating quantitative trait inheritance. Then, employing whole-genome resequencing (WGR), a TKS genetic linkage map of 12,680 bin markers comprising 322,439 SNPs was generated. Based on the genetic map and NR content of the F1 population, six quantitative trait loci (QTLs) for NR content with LOD > 4.0 were identified on LG01/Chr01 and LG06/Chr06. Of them, the 2.17 Mb genomic region between qHRC-C6-1 and qHRC-C6-2 on ChrA06, with 65.62% PVE in total, was the major QTL region. In addition, the six QTLs have significant additive genetic effects on NR content and could be used to develop markers for marker-assisted selection (MAS) in TKS with a high NR content. Conclusion This work constructed the first high-density TKS genetic map and identified the QTLs and genomic regions controlling the NR content, which provides useful information for fine mapping, map-based cloning, and MAS in TKS.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Construction of the first high-density SNP genetic map and identification of QTLs for the natural rubber content in Taraxacum kok-saghyz Rodin

et al. 2023

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“…Guo et al [29] found that Miscanthus sacchariflorus had resistance to low acid and heavy metal toxicity, but Miscanthus sacchariflorus likes warm, moist, and semi-shady environments. Zhang et al [30] pointed out that Taraxacum koksaghyz had good tolerance to Pb and Cd stress, but its biomass was small. Taraxacum koksaghyz generally grew in alkaline environments.…”

Section: Discussionmentioning

confidence: 99%

Adaptability of Koenigia mollis to an Acid Tin Mine Wasteland in Lianghe County in Yunnan Province

Deng

Xia

et al. 2023

Sustainability

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To explore the potential of Koenigia mollis as a pioneer plant in acid tin mine wasteland, Koenigia mollis plants and the corresponding rhizosphere soils in different areas in Lianghe County, Yunnan Province were collected, and their chemical properties and heavy metals contents were determined., the adaptability of the plant to the barren tailing environment and its acid resistance and tolerance to heavy metal such as Cu (Cu, CAS. No. 7144-37-8), Cd (Cd, CAS. No. 7440-43-9) and Pb (Pb, CAS. No. 10099-74-8) pollution were analyzed. Results showed that Koenigia mollis growth was normal. The pH value in rhizosphere soils was 3.74–4.30, which was strongly acidic. The organic matter (OM), total nitrogen (TN) (N, CAS. No. 7727-37-9), available potassium (AK) (K, CAS. No. 7440-09-7), and available phosphorus (AP) (P, CAS. No. 7723-14-0) contents in soils of the research area were in low levels. The total contents of Cu, Cd, and Pb in the soil of the research area exceeded the pollution risk screening value for the national risk control standard of soil environmental quality, indicating that Koenigia mollis has a certain resistance to acid and heavy metal pollution. In addition, Koenigia mollis has strong transport and enrichment capacity for Cu, Cd, and Pb and therefore has potential as a pioneer phytoremediation plant for acid tin mine wastelands and a remediated plant for agricultural land around metal mining areas.

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“…The D population showed a closer genetic relationship with the C population, compared with the AB populations. A comparative analysis of the genomes of different populations showed differences in the genes involved in their adaptation to different environmental conditions [42]. In another study, high-quality genomes of the rubber-bearing TKS and the medicinal plant T. mongolicum Hand.-Mazz.…”

Section: Molecular Genetic and Genome-wide Studiesmentioning

confidence: 98%

“…In 2021, a total of 524,812 SNPs were found, based on analysis of whole genome sequencing results [42]. These SNPs were used to construct the population structure, genetic diversity, and evolutionary analysis of 58 wild TKS plants collected from four different regions.…”

Section: Molecular Genetic and Genome-wide Studiesmentioning

confidence: 99%

Molecular Genetic Research and Genetic Engineering of Taraxacum kok-saghyz L.E. Rodin

Kuluev

Uteulin

Bari

et al. 2023

Plants

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Natural rubber (NR) remains an indispensable raw material with unique properties that is used in the manufacture of a large number of products and the global demand for it is growing every year. The only industrially important source of NR is the tropical tree Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg., thus alternative sources of rubber are required. For the temperate zone, the most suitable source of high quality rubber is the Russian (Kazakh) dandelion Taraxacum kok-saghyz L.E. Rodin (TKS). An obstacle to the widespread industrial cultivation of TKS is its high heterozygosity, poor growth energy, and low competitiveness in the field, as well as inbreeding depression. Rapid cultivation of TKS requires the use of modern technologies of marker-assisted and genomic selection, as well as approaches of genetic engineering and genome editing. This review is devoted to describing the progress in the field of molecular genetics, genomics, and genetic engineering of TKS. Sequencing and annotation of the entire TKS genome made it possible to identify a large number of SNPs, which were subsequently used in genotyping. To date, a total of 90 functional genes have been identified that control the rubber synthesis pathway in TKS. The most important of these proteins are part of the rubber transferase complex and are encoded by eight genes for cis-prenyltransferases (TkCPT), two genes for cis-prenyltransferase-like proteins (TkCPTL), one gene for rubber elongation factor (TkREF), and nine genes for small rubber particle proteins (TkSRPP). In TKS, genes for enzymes of inulin metabolism have also been identified and genome-wide studies of other gene families are also underway. Comparative transcriptomic and proteomic studies of TKS lines with different accumulations of NR are also being carried out, which help to identify genes and proteins involved in the synthesis, regulation, and accumulation of this natural polymer. A number of authors already use the knowledge gained in the genetic engineering of TKS and the main goal of these works is the rapid transformation of the TKS into an economically viable rubber crop. There are no great successes in this area so far, therefore work on genetic transformation and genome editing of TKS should be continued, considering the recent results of genome-wide studies.

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Genetic diversity and evolutionary patterns of Taraxacum kok‐saghyz Rodin

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 8 publications

References 57 publications

Construction of the first high-density SNP genetic map and identification of QTLs for the natural rubber content in Taraxacum kok-saghyz Rodin

Construction of the first high-density SNP genetic map and identification of QTLs for the natural rubber content in Taraxacum kok-saghyz Rodin

Adaptability of Koenigia mollis to an Acid Tin Mine Wasteland in Lianghe County in Yunnan Province

Molecular Genetic Research and Genetic Engineering of Taraxacum kok-saghyz L.E. Rodin

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