Genetic Diversity and Population Genetics of Mosquitoes (Diptera: Culicidae: <i>Culex</i> spp.) from the Sonoran Desert of North America

Pfeiler, Edward; Flores-López, Carlos A.; Mada-Vélez, Jesús Gerardo; Escalante-Verdugo, Juan; Markow, Therese A.

doi:10.1155/2013/724609

Cited by 22 publications

(19 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S2 Table shows the posterior distribution of parameters for Approximate Bayesian Computation (ABC) analysis using the DIYABC software [46]. The estimated mutation rate under the best-fit scenario was 9.5x10 -6 , and falls within the range of microsatellite mutation rates estimated for other Diptera [50,51]. …”

Section: Resultsmentioning

confidence: 95%

Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses

et al. 2017

View full text Add to dashboard Cite

BackgroundAedes aegypti, commonly known as “the yellow fever mosquito”, is of great medical concern today primarily as the major vector of dengue, chikungunya and Zika viruses, although yellow fever remains a serious health concern in some regions. The history of Ae. aegypti in Brazil is of particular interest because the country was subjected to a well-documented eradication program during 1940s-1950s. After cessation of the campaign, the mosquito quickly re-established in the early 1970s with several dengue outbreaks reported during the last 30 years. Brazil can be considered the country suffering the most from the yellow fever mosquito, given the high number of dengue, chikungunya and Zika cases reported in the country, after having once been declared “free of Ae. aegypti”.Methodology/Principal findingsWe used 12 microsatellite markers to infer the genetic structure of Brazilian Ae. aegypti populations, genetic variability, genetic affinities with neighboring geographic areas, and the timing of their arrival and spread. This enabled us to reconstruct their recent history and evaluate whether the reappearance in Brazil was the result of re-invasion from neighboring non-eradicated areas or re-emergence from local refugia surviving the eradication program. Our results indicate a genetic break separating the northern and southern Brazilian Ae. aegypti populations, with further genetic differentiation within each cluster, especially in southern Brazil.Conclusions/SignificanceBased on our results, re-invasions from non-eradicated regions are the most likely scenario for the reappearance of Ae. aegypti in Brazil. While populations in the northern cluster are likely to have descended from Venezuela populations as early as the 1970s, southern populations seem to have derived more recently from northern Brazilian areas. Possible entry points are also revealed within both southern and northern clusters that could inform strategies to control and monitor this important arbovirus vector.

show abstract

Section: Resultsmentioning

confidence: 95%

Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses

et al. 2017

View full text Add to dashboard Cite

show abstract

“…; Pfeiler et al . ). The time estimated for the founding of Asian populations assuming an average of 10 generations per year is 1280–1770 generations or ~150 years ago (Table S1, Supporting information), setting priors for leaving Africa at 4500–6000 generations ago (~500 years ago) in agreement with historical records, suggesting the New World populations were founded by trade between the New World and Old World in the 15th and 16th centuries.…”

Section: Resultsmentioning

confidence: 97%

“…; Pfeiler et al . ). Additional details of the diyabc analysis can be found in Tables S1 and S2 (Supporting information).…”

Section: Methodsmentioning

confidence: 97%

Global genetic diversity ofAedes aegypti

et al. 2016

View full text Add to dashboard Cite

Mosquitoes, especially Aedes aegypti, are becoming important models for studying invasion biology. We characterized genetic variation at 12 microsatellite loci in 79 populations of Ae. aegypti, from 30 countries in six continents and used them to infer historical and modern patterns of invasion. Our results support the two subspecies Ae. aegypti formosus and Ae. aegypti aegypti as genetically distinct units. Ae. aegypti aegypti populations outside Africa are derived from ancestral African populations and are monophyletic. The two subspecies co-occur in both East Africa (Kenya) and West Africa (Senegal). In rural/forest settings (Rabai District of Kenya) the two subspecies remain genetically distinct whereas in urban settings they introgress freely. Populations outside Africa are highly genetically structured likely due to a combination of recent founder effects, discrete discontinuous habitats, and low migration rates. Ancestral populations in sub-Saharan Africa are less genetically structured, as are the populations in Asia. Introduction of Ae. aegypti to the New World coinciding with trans-Atlantic shipping in the 16th to 18th Centuries was followed by its introduction to Asia in the late 19th Century from the New World or from now extinct populations in the Mediterranean Basin. Aedes mascarensis is a genetically distinct sister species to Ae. aegypti s.l.. This study provides a reference database of genetic diversity that can be used to determine the likely origin of new introductions that occur regularly for this invasive species. The genetic uniqueness of many populations and regions has important implications for attempts to control Ae. aegypti, especially for methods using genetic modification of populations.

show abstract

“…These species are part of the Cx. pipiens complex, which is known to be difficult to distinguish using standard barcoding methods (Laurito et al 2013; Pfeiler et al 2013; Batovska et al 2016). In comparison to COI as a marker in the same specimens/species (Batovska et al 2016), ITS2 has similar resolution, with both markers successfully distinguishing all species with the exception of the Cx.…”

Section: Discussionmentioning

confidence: 99%

Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2

Batovska

Cogan

Lynch

et al. 2017

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Internal Transcribed Spacer 2 (ITS2) is a popular DNA barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. With next-generation sequencing (NGS) it is possible to sequence all gene variants despite the presence of single nucleotide polymorphisms (SNPs), insertions/deletions (indels), homopolymeric regions, and microsatellites. Our aim was to compare the performance of Sanger sequencing and NGS amplicon sequencing in characterizing ITS2 in 26 mosquito species represented by 88 samples. The suitability of ITS2 as a DNA barcoding marker for mosquitoes, and its allelic diversity in individuals and species, was also assessed. Compared to Sanger sequencing, NGS was able to characterize the ITS2 region to a greater extent, with resolution within and between individuals and species that was previously not possible. A total of 382 unique sequences (alleles) were generated from the 88 mosquito specimens, demonstrating the diversity present that has been overlooked by traditional sequencing methods. Multiple indels and microsatellites were present in the ITS2 alleles, which were often specific to species or genera, causing variation in sequence length. As a barcoding marker, ITS2 was able to separate all of the species, apart from members of the Culex pipiens complex, providing the same resolution as the commonly used Cytochrome Oxidase I (COI). The ability to cost-effectively sequence hypervariable markers makes NGS an invaluable tool with many applications in the DNA barcoding field, and provides insights into the limitations of previous studies and techniques.

show abstract

Genetic Diversity and Population Genetics of Mosquitoes (Diptera: Culicidae: Culex spp.) from the Sonoran Desert of North America

Cited by 22 publications

References 56 publications

Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses

Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses

Global genetic diversity ofAedes aegypti

Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2

Contact Info

Product

Resources

About