Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers

Güngör, Hüseyin; İlhan, Emre; KASAPOĞLU, Ayşe Gül; Filiz, Ertuğrul; Türkoğlu, Aras; Valchev, Dragomir; Valcheva, D.; Haliloğlu, Kamil; Dumlupınar, Ziya

doi:10.15832/ankutbd.886221

Cited by 2 publications

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“…In order to maintain yield in extremely dry and salty areas, it has therefore been suggested to cultivate salt-tolerant barley genotypes. Breeding is crucial for creating toolkits of salt-tolerant crop genotypes and for implementing growth, physiological, and yield features in the field [ 33 ]. According to El-Khalifa et al [ 34 ], barley is an essential crop in Egypt and takes up 76.9% of freshly reclaimed land.…”

Section: Introductionmentioning

confidence: 99%

Agro-morphological, biochemical, and molecular markers of barley genotypes grown under salinity stress conditions

Ghonaim,

Attya,

Aly

et al. 2023

BMC Plant Biol

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The aim of this study was to evaluate the impact of salt stress on morphological, yield, biochemical, and molecular attributes of different barley genotypes. Ten genotypes were cultivated at Fayoum Research Station, El-Fayoum Governorate, Egypt, during two seasons (2020–2021 and 2021–2022), and they were exposed to two different salt concentrations (tap water as a control and 8000 ppm). The results showed that genotypes and salt stress had a significant impact on all morphological and physiological parameters. The morphological parameters (plant height) and yield attributes (spike length, number of grains per spike, and grain yield per plant) of all barley genotypes were significantly decreased under salt stress as compared to control plants. Under salt stress, the total soluble sugars, proline, total phenol, total flavonoid, ascorbic acid, malondialdehyde, hydrogen peroxide, and sodium contents of the shoots of all barley genotypes significantly increased while the potassium content decreased. L1, which is considered a sensitive genotype was more affected by salinity stress than the tolerance genotypes L4, L6, L9, and Giza 138. SDS-PAGE of seed proteins demonstrated high levels of genetic variety with a polymorphism rate of 42.11%. All genotypes evaluated revealed significant variations in the seed protein biochemical markers, with new protein bands appearing and other protein bands disappearing in the protein patterns of genotypes cultivated under various conditions. Two molecular marker techniques (SCoT and ISSR primers) were used in this study. Ten Start Codon Targeted (SCoT) primers exhibited a total of 94 fragments with sizes ranging from 1800 base pairs to 100 base pairs; 29 of them were monomorphic, and 65 bands, with a polymorphism of 62.18%, were polymorphic. These bands contained 21 unique bands (9 positive specific markers and 12 negative specific markers). A total of 54 amplified bands with molecular sizes ranging from 2200 to 200 bp were produced using seven Inter Simple Sequence Repeat (ISSR) primers; 31 of them were monomorphic bands and 23 polymorphic bands had a 40.9% polymorphism. The techniques identified molecular genetic markers associated with salt tolerance in barley crop and successfully marked each genotype with distinct bands. The ten genotypes were sorted into two main groups by the unweighted pair group method of arithmetic averages (UPGMA) cluster analysis based on molecular markers and data at a genetic similarity coefficient level of 0.71.

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Section: Introductionmentioning

confidence: 99%

Agro-morphological, biochemical, and molecular markers of barley genotypes grown under salinity stress conditions

Ghonaim,

Attya,

Aly

et al. 2023

BMC Plant Biol

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Genetic diversity analysis of lesser burnet (Sanguisorba minor Scop.) genotypes collected from Erzurum Province, Türkiye Using inter-simple sequence repeat (ISSR) markers

Karataş,

Kasapoğlu,

Güllap

et al. 2024

Genet Resour Crop Evol

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Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers

Abstract: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.

Cited by 2 publications

References 50 publications

Agro-morphological, biochemical, and molecular markers of barley genotypes grown under salinity stress conditions

Agro-morphological, biochemical, and molecular markers of barley genotypes grown under salinity stress conditions

Genetic diversity analysis of lesser burnet (Sanguisorba minor Scop.) genotypes collected from Erzurum Province, Türkiye Using inter-simple sequence repeat (ISSR) markers

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