Genetic diversity and population structure in Portuguese goat breeds

Sousa, Carolina Bruno de; Martínez-Martínez, A.; Ginja, Catarina; Santos-Silva, F.; Carolino, Inês; Delgado, Juan Vicente; Gama, L. T.

doi:10.1016/j.livsci.2010.06.159

Cited by 34 publications

(28 citation statements)

References 48 publications

(55 reference statements)

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“…They reported mixedbreed individuals caused by occasional migration from the two neighboring countries to Saudi Arabia. Our results confirm the results of previous studies that have found that microsatellite markers can assign goats to their breeds (Araújo et al, 2006;Bruno-de-Sousa et al, 2010). In conclusion, the results of a correspondence analysis based on 17 MS genotypes were clearly successful in distinguishing the KSA breeds from each other and from Somali goat breed.…”

Section: Discussionsupporting

confidence: 91%

Use of microsatellite markers to assign goats to their breeds

2015

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ABSTRACT. We investigated the potential of 17 microsatellite markers for assigning Saudi goat individuals to their breeds. Three local breeds, Bishi, Jabali, and Tohami were genotyped using these markers, and Somali goats were used as a reference breed. The majority of alleles were shared between the breeds, except for some that were specific to each breed. The Garza-Williamson index was lowest in the Bishi breed, indicating that a recent bottleneck event occurred. The overall results assigned the goat individuals (based on their genotypes) to the same breeds from which they were sampled, except in a few cases. The individuals' genotypes were sufficient to provide a clear distinction between the Somali goat breed and the others. In three factorial dimensions, the results of a correspondence analysis indicated that the total variation for the first and second factors was 48.85 and 31.43%, respectively. Consequently, Jabali, Bishi, and Tohami goats were in separate groups. The Jabali goat was closely related to the Bishi goat. Somali goats were distinguished from each other and from individuals of the other three goat breeds. The markers were successful in assigning individual goats to their breeds, based on the likelihood of a given individual's genotype.

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Section: Discussionsupporting

confidence: 91%

Use of microsatellite markers to assign goats to their breeds

2015

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“…The PIC values obtained in this study were similar to or higher than those reported for most of the loci [32][33][34][35][36][37] . Therefore the loci examined in this study were highly informative.…”

Section: Discussionsupporting

confidence: 86%

Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Gürler¹,

Bozkaya²

2013

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the genetic variability of three goat populations raised in the Southeastern region of Turkey by using microsatellite loci defined in gazelle (Gazella granti). DNA was isolated from 120 blood samples collected from goats raised in Sanliurfa (n = 40), Kilis (n = 40) and Siirt (n = 40) provinces. Eight microsatellite loci (33HDZ8, 33HDZ290, 33HDZ433, 33HDZ496, 33HDZ593, 33HDZ692, 33HDZ749, 33HDZ974) were used for amplification with polymerase chain reaction (PCR). Fragment lengths of the amplifed loci were analysed using a capillary electrophoresis system. Six of the eight loci (75%) were successfully amplified and four (66.7%) of them were polymorphic. Three of the polymorphic loci (33HDZ496, 33HDZ749 ve 33HDZ974) were used for estimating genetic parameters. A total of 40 alleles were detected. Number of alleles and polymorphism information contents (PIC) for the loci 33HDZ496, 33HDZ749 and 33HDZ974 Keywords: Goat, Microsatellite, Genetic diversity Güney Doğu Anadolu Bölgesi'nde Yetiştirilen Üç Keçi Populasyonunda Genetik Çeşitlilik ÖzetBu çalışma bir ceylan türünde (Gazella granti) tanımlanmış sekiz mikrosatelit lokusunu kullanarak Güneydoğu Anadolu Bölgesinde yetiştirilen üç keçi populasyonunu genetik çeşitlilik yönünden değerlendirmek amacıyla yapılmıştır. Bu amaçla, Şanlıurfa (n = 40), Kilis (n = 40) ve Siirt (n = 40) illerinde yetiştirilen üç keçi populasyonundan toplam 120 adet kan örneği alınmış ve bu örneklerden DNA izole edilmiştir. İzole edilen DNA'lar sekiz mikrosatelit lokusuna (33HDZ8, 33HDZ290, 33HDZ433, 33HDZ496, 33HDZ593, 33HDZ692, 33HDZ749, 33HDZ974) ait primerler kullanılarak PCR ile çoğaltılmış ve PCR ürünleri kapillar elektroforeze tabi tutularak fragman uzunluğu analizi gerçekleştirilmiştir. Sekiz lokustan altısı (%75) PCR ile amplifiye olmuş ve bunlardan dördü (%66.7) polimorfik bulunmuştur. Polimorfik lokusların üçü (33HDZ496, 33HDZ749 ve 33HDZ974) genetik parametrelerin hesaplanmasında kullanılmıştır. Çalışmada toplam 40 allel tespit edilmiştir. 33HDZ496, 33HDZ749 ve 33HDZ974 lokusları için sırasıyla 13, 15 ve 12 allel gözlenmiş olup, herbir lokus için hesaplanan polimorfizm bilgi içeriği (PIC) aynı sırayla 0.836, 0.821 ve 0.817 olarak belirlenmiştir. Şanlıurfa, Kilis ve Siirt populasyonları için ortalama allel sayıları sırasıyla 10.33±1.15, 10.67±2.08 ve 11.33±0.58, etkili allel sayıları ise yine aynı sıra ile 6.131±1.42, 5.823±0.23 ve 5.870±0.75 olarak bulunmuştur. Şanlıurfa, Kilis ve Siirt populasyonları için ortalama gözlenen heterozigotluk oranları sırasıyla 0.827±0.083, 0.808±0.044 ve 0.895±0.069, beklenen heterozigotluk oranları ise yine aynı sıra ile 0.843±0.043, 0.841±0.007 ve 0.838±0.023 olarak belirlenmiştir. 33HDZ496, 33HDZ749 ve 33HDZ974 lokusları için hesaplanan FIS değerleri sırasıyla 0.0164, 0.0245 ve 0.0240 olarak bulunmuştur. Tüm populasyonlar için hesaplanan FST değeri (0.009) incelenen populasyonda gözlenen genetik çeşitliliğin %99'unun bireyler arasındaki farklılıktan kaynaklandığını göstermiştir. Çalışma sonuçları incelenen ke...

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“…Microsatellite markers are very useful for population genetic analysis, as they are highly informative and can successfully elucidate the relationships between both individuals and populations. These neutral markers have been extensively used in genetic characterization of several goat breeds, including those from Portugal (Bruno-de-Sousa et al 2011) andBrazil (Araú jo et al 2006;Menezes et al 2006;Oliveira et al 2007), as well as in large-scale studies covering wider geographical areas (Cañ on et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Drift across the Atlantic: genetic differentiation and population structure in Brazilian and Portuguese native goat breeds

Ribeiro

Sousa

Martínez-Martínez

et al. 2011

J Animal Breeding Genetics

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Brazilian goat breeds are believed to derive mainly from animals brought by Portuguese settlers since the 16th century. We used microsatellite markers in a sample of 436 animals to study genetic variability and differentiation of the six Portuguese (PT) and six Brazilian (BR) goat breeds currently recognized in the two countries. These breeds were also compared with an outgroup represented by a sample of Alpine (ALP) goats. The effective number of alleles and allelic richness were slightly higher in PT than in BR breeds. The global F(ST) was nearly 0.11 when PT and BR breeds were considered, with a mean pairwise F(ST) of about 0.03 among PT breeds, 0.07 among BR breeds and 0.15 between PT and BR breeds. The dendrogram illustrating relationships between populations and the correspondence analysis indicate the existence of two very distinct clusters, corresponding to the countries of origin of the breeds studied, which are nearly equidistant from the Alpine outgroup. The analysis with structure confirmed the separation between PT and BR breeds but suggests that some BR breeds, especially Graúna and Canindé, may share a common ancestry with PT breeds. The divergence observed between PT and BR breeds may result from founder effects and genetic drift but could also reflect the introduction in Brazil of goats originating from other regions, e.g., West Africa.

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Genetic diversity and population structure in Portuguese goat breeds

Cited by 34 publications

References 48 publications

Use of microsatellite markers to assign goats to their breeds

Use of microsatellite markers to assign goats to their breeds

Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Drift across the Atlantic: genetic differentiation and population structure in Brazilian and Portuguese native goat breeds

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