Genetic diversity and population structure of<i>Synthesium pontoporiae</i>(Digenea, Brachycladiidae) linked to its definitive host stocks, the endangered Franciscana dolphin,<i>Pontoporia blainvillei</i>(Pontoporiidae) off the coast of Brazil and Argentina

Marigo, Juliana; Cunha, Haydée A.; Bertozzi, Carolina Pacheco; Souza, Shirley Pacheco de; Rosas, Fernando C. W.; Cremer, Marta Jussara; Barreto, André S.; Oliveira, Larissa Rosa de; Cappozzo, Humberto Luis; Valente, Ana Luísa; Santos, Cláudia Portes; Vicente, Ana Carolina Paulo

doi:10.1017/s0022149x13000540

Cited by 13 publications

(9 citation statements)

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“…As far as we are aware, there is a single study that has investigated the phylogeography and genetic structure of a digenean from cetaceans. Marigo et al [16] described the genetic structure of Synthesium pontoporiae , which is specific to the Franciscana dolphin, Pontoporia blainvillei . This largely sedentary cetacean has a relatively restricted geographic range occurring along the temperate coasts of southern Brazil, Uruguay and Argentina.…”

Section: Introductionmentioning

confidence: 99%

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Long-Distance Travellers: Phylogeography of a Generalist Parasite, Pholeter gastrophilus, from Cetaceans

et al. 2017

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We studied the phylogeography and historical demography of the most generalist digenean from cetaceans, Pholeter gastrophilus, exploring the effects of isolation by distance, ecological barriers and hosts’ dispersal ability on the population structure of this parasite. The ITS2 rDNA, and the mitochondrial COI and ND1 from 68 individual parasites were analysed. Worms were collected from seven oceanic and coastal cetacean species from the south western Atlantic (SWA), central eastern Atlantic, north eastern Atlantic (NEA), and Mediterranean Sea. Pholeter gastrophilus was considered a single lineage because reciprocal monophyly was not detected in the ML cladogram of all individuals, and sequence variability was <1% for mtDNA and 0% for ITS2. These results rule out a recent suggestion that P. gastrophilus would actually be a cryptic-species complex. The genetic cohesion of P. gastrophilus could rely on the extensive exploitation of wide-ranging and highly mobile cetaceans, with a putative secondary role, if any, of intermediate hosts. Unique haplotypes were detected in SWA and NEA, and an AMOVA revealed significant population structure associated to the genetic variation in these regions. The Equator possibly acts as a significant geographical barrier for cetacean movements, possibly limiting gene flow between northern and southern populations of P. gastrophilus. A partial Mantel tests revealed that the significant isolation of NEA populations resulted from geographic clustering. Apparently, the limited mobility of cetaceans used by P. gastrophilus as definitive hosts in this region, coupled with oceanographic barriers and a patchy distribution of potential intermediate hosts could contribute to significant ecological isolation of P. gastrophilus in NEA. Rather unexpectedly, no genetic differentiation was found in the Mediterranean samples of this parasite. Historical demographic analyses suggested a recent population expansion of P. gastrophilus in the Atlantic Ocean, perhaps linked to initial association and subsequent spreading in cetaceans.

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Section: Introductionmentioning

confidence: 99%

“…pontoporiae , which suggests that the dispersal capacity of the (unknown) intermediate hosts would most likely have overcome the low vagility of definitive hosts and contributed to the genetic mixing of S . pontoporiae populations [16]. …”

Section: Introductionmentioning

confidence: 99%

Long-Distance Travellers: Phylogeography of a Generalist Parasite, Pholeter gastrophilus, from Cetaceans

et al. 2017

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“…The cytochromo oxidase subunit I (COI) is part of a complex gene encoding of transmembrane proteins, involved in the electrical transport and catalysis of the respiratory chain of eukaryotic organisms and has been the subject respiratoria de organismos eucariotas y ha sido motivo de estudios poblacionales de variabilidad genética por su universalidad e importancia evolutiva (9). El ADNmt presenta características como su herencia matrilineal, es haploide, presenta una alta tasa de evolución y contiene regiones conservadas y variables, que lo han convertido en una herramienta utilizada en varios estudios (10)(11)(12). Por lo anterior, el propósito de la presente investigación fue utilizar el COI como marcador molecular para estimar la diversidad genética en cinco islas de manglar en Tumaco, Colombia.…”

Section: Introductionunclassified

“…of population genetic variability studies due to its universality and evolutionary importance (9). The mtDNA has characteristics such as its matrilineal inheritance, it is haploid, has a high rate of evolution and includes conserved and variable regions, which has made it a tool used in several studies (10)(11)(12). Therefore, the purpose of this research was to use COI as molecular marker to estimate genetic diversity in five mangrove islands in Tumaco, Colombia.…”

Section: Extracción De Adnmentioning

confidence: 99%

Estimación de la diversidad genética de Anadara tuberculosa en cinco manglares de Tumaco, utilizando la enzima citocromo oxidasa I

2016

Rev MVZ Córdoba

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Objective. To estimate the genetic diversity of the Anadara tuberculosa en five mangrove swams of Tumaco, Nariño, Colombia using as a mitocondrial molecular marker the cytochromo oxidase sub-unit I (COI). Materials and methods. A total of 50 individuals were collected from the San Jorge, La Tiburonera, El Pajal, La Playa y Bajito Vaquería mangrove swamps, randomly selecting 10 specimens of each zone. The tissue sample was worked with absolute alcohol at ambient temperature in microtubes. DNA was extracted, and the mitocondrial DNA was amplified using the PCR technique (polymerase chain reaction). The amplified and quantified products of PCR were sequenced on both sides (Macrogen). Each one of the obtained sequences was edited and aligned. Later, the parameters of genetic diversity (haplotypical and nucleotidical) were measured, and the analysis of distribution between frequency pairs (Mistmach distribution) was elaborated. Finally, the analysis of nucleotidic variation and population structure (AMOVA) was completed. Results. The amplified product gene weighed 710 bp. The haplotypical diversity reported for all the populations was high (0.683±0.060) and the reported nucleotídical diversity was low for all the populations (0.040±0.020). The AMOVA results indicate that the variance amongst populations is low (4.20%) and that the variance within populations is high (95.80%). Conclusions. The studied populations are not structured and although there is a decrease of natural banks, the genetic diversity is high.

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“…The molecular data affi rmed that samples of B. atlanticum were conspecifi c in spite of host species. Marigo et al (2013) genetically distinguished the host cetacean species (Pontoporia blainvillei) infected by Synthesium pontoporiae from Brazil and Argentina using ITS-1, ITS-2 rDNA and mitochondrial ND3 and COI gene sequences. Any intra-specifi c differences were not observed among ITS-1 and ITS-2 reigon of S. pontoporiae.…”

Section: Brachycladiidae (Odhner 1905)mentioning

confidence: 99%

A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

et al. 2015

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SummaryThe rDNA region of eukaryotes has the immense potential to resolve the evolutionary and phylogeny problems using molecular markers. As evident from the present review, ITS region data is considered for interpretation of inter and intra-specifi c variations of 136 studies of 33 families including 78 genus and 114 species affecting individuals worldwide. Along with ITS-1 and ITS-2 region in 29 studies 18S region, in 38 studies 28S region and in 43 studies mitochondrial genes (COI and NDI) were also analyzed. Three new genera (Allobilharzia gen. nov., Caulanus gen. nov., and Latuterus gen. nov.) and 49 new species were discovered. Only 47 studies expressed variations at intra-specifi c and inter-specifi c level in complete ITS region, ITS-1 and ITS-2 rDNA sequences due to differences in nucleotide positions. According to the fi ndings ITS region is more reliable and precise marker for demarcation and identifi cation of species in combination of other DNA markers. Major studies were involved around the parasites of families Fasciolidae, Schistosomatidae, Opisthorchidae, Paragonimidae and Paramphistomidae, Clinostomidae, Diplostomidae, Haploporidae, among others infecting humans, farm animals, birds, fi shes, reptiles and amphibians on the clinical basis. In future, molecular and bioinformatics aspects based on genetic variations will lead to explore the untouched areas of trematodes.

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Genetic diversity and population structure ofSynthesium pontoporiae(Digenea, Brachycladiidae) linked to its definitive host stocks, the endangered Franciscana dolphin,Pontoporia blainvillei(Pontoporiidae) off the coast of Brazil and Argentina

Cited by 13 publications

References 50 publications

Long-Distance Travellers: Phylogeography of a Generalist Parasite, Pholeter gastrophilus, from Cetaceans

Long-Distance Travellers: Phylogeography of a Generalist Parasite, Pholeter gastrophilus, from Cetaceans

Estimación de la diversidad genética de Anadara tuberculosa en cinco manglares de Tumaco, utilizando la enzima citocromo oxidasa I

A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

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