Genetic diversity and population structure of an important wild berry crop

Zoratti, Laura; Palmieri, L.; Jaakola, Laura; Häggman, Hely

doi:10.1093/aobpla/plv117

Cited by 22 publications

(20 citation statements)

References 39 publications

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“…Our results would not entirely support this hypothesis, as we found no correlation between diversity and latitude as would be expected with receding ice sheets [55]. Genetic differentiation as a result of geographic isolation has been found in bilberry (Vaccinium myrtillus L.) throughout Scandinavia, and the same may be true for lowbush blueberry here in North America but greater geographical separation than is reported here is likely to be a necessity [56]. The large geographic range, outcrossing, and animal seed dispersal traits associated with lowbush blueberry likely contributes to genetic diversity [57].…”

Section: Discussioncontrasting

confidence: 84%

Genetic Diversity of Lowbush Blueberry throughout the United States in Managed and Non-Managed Populations

Beers¹,

Rowland

Drummond

2019

Agriculture

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Expressed sequenced tagged-polymerase chain reaction (EST-PCR) molecular markers were used to evaluate the genetic diversity of lowbush blueberry across its geographic range and to compare diversity among four paired managed/non-managed populations. Seventeen populations were sampled in a north–south transect throughout the eastern United States with 27 km to 1600 km separating populations. The majority of genetic variation was found within populations (75%) with each population genetically unique (p ≤ 0.0001) with the exception of the Jonesboro, ME, and Lubec, ME, populations. The effects of management for commercial fruit harvesting on genetic diversity were investigated in four locations in Maine with paired managed and non-managed populations. Significant differences were found between the populations indicating that commercial management for fruit production influences the diversity of lowbush blueberries in the landscape, even though planting does not occur. Forests are harvested and the existing understory blueberry plants become established.

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Section: Discussioncontrasting

confidence: 84%

Genetic Diversity of Lowbush Blueberry throughout the United States in Managed and Non-Managed Populations

Beers¹,

Rowland

Drummond

2019

Agriculture

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“…This analysis, designed to relate species composition to different predictive variables (Ter Braak 1986), has been successfully used to describe relationships between environmental or phenotypical variables and genetic composition (Angers et al 1999; Dell’Acqua et al 2014; Zoratti et al 2015). The analysis was based on a disease levels/genotype matrix.…”

Section: Methodsmentioning

confidence: 99%

Frequency of a natural truncated allele of MdMLO19 in the germplasm of Malus domestica

et al. 2017

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Podosphaera leucotricha is the causal agent of powdery mildew (PM) in apple. To reduce the amount of fungicides required to control this pathogen, the development of resistant apple cultivars should become a priority. Resistance to PM was achieved in various crops by knocking out specific members of the MLO gene family that are responsible for PM susceptibility (S-genes). In apple, the knockdown of MdMLO19 resulted in PM resistance. However, since gene silencing technologies such as RNAi are perceived unfavorably in Europe, a different approach that exploits this type of resistance is needed. This work evaluates the presence of non-functional naturally occurring alleles of MdMLO19 in apple germplasm. The screening of the re-sequencing data of 63 apple individuals led to the identification of 627 single nucleotide polymorphisms (SNPs) in five MLO genes (MdMLO5, MdMLO7, MdMLO11, MdMLO18, and MdMLO19), 127 of which were located in exons. The T-1201 insertion of a single nucleotide in MdMLO19 caused the formation of an early stop codon, resulting in a truncated protein lacking 185 amino acids, including the calmodulin-binding domain. The presence of the insertion was evaluated in 115 individuals. It was heterozygous in 64 and homozygous in 25. Twelve of the 25 individuals carrying the insertion in homozygosity were susceptible to PM. After barley, pea, cucumber, and tomato, apple would be the fifth species for which a natural non-functional mlo allele has been found.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-016-0610-8) contains supplementary material, which is available to authorized users.

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“…Berries from the Nordic forests are very popular on the world market (Zoratti et al 2015) because their extracts have been used as food and health supplements (Basu et al 2010;Pascu et al 2013) as well as functional ingredients in cosmetics production by a number of industries (Benzie and Wachtel-Galor 2011;Moze et al 2011).…”

Section: Berries and Bilberriesmentioning

confidence: 99%

Solvent and Rotating Bed Reactor Extraction with One- and Two-Phase Solvents Applied to Bilberries (Vaccinium myrtillus) for Isolating Valuable Antioxidants

et al. 2020

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Extraction of antioxidants from bilberries using solvent extraction and the novel rotating bed reactor (RBR) both with one-phase (ethanol and water) and two-phase (ethanol and water +salt) solvents was studied. Solids, ethanol, and temperature settings in an experimental design were monitored for 1 h. The measured responses were (1) polyphenol concentration, (2) visible-near infrared spectra, and (3) HPLC measurement. The (1) responses were used for making response surfaces in time and the spectra (2) could confirm these results. The HPLC results (3) confirmed the results of 1 and 2 but were found unsuitable for online monitoring. The RBR was better than traditional extraction and 16 min sufficed. The response surfaces showed an optimal concentration of ethanol, temperatures above 50°C gave the best results, and high loads of solid were beneficial. Two-phase extraction was less efficient. The methodology could be transferred to larger scale extraction systems to improve yield and save on reagents/energy cost.

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Genetic diversity and population structure of an important wild berry crop

Cited by 22 publications

References 39 publications

Genetic Diversity of Lowbush Blueberry throughout the United States in Managed and Non-Managed Populations

Genetic Diversity of Lowbush Blueberry throughout the United States in Managed and Non-Managed Populations

Frequency of a natural truncated allele of MdMLO19 in the germplasm of Malus domestica

Solvent and Rotating Bed Reactor Extraction with One- and Two-Phase Solvents Applied to Bilberries (Vaccinium myrtillus) for Isolating Valuable Antioxidants

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